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Protein

ATP-dependent RNA helicase mtr4

Gene

mtr4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the TRAMP complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates (By similarity). Required for heterochromatic gene silencing at centromeric repeats by either exosome- or RNAi-mediated degradation of heterochromatic transcripts.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2278ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-6791226. Major pathway of rRNA processing in the nucleolus.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase mtr4 (EC:3.6.4.-)
Gene namesi
Name:mtr4
ORF Names:SPAC6F12.16c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.16c.
PomBaseiSPAC6F12.16c. mtr4.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: PomBase
  • nucleus Source: PomBase
  • TRAMP complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11171117ATP-dependent RNA helicase mtr4PRO_0000102098Add
BLAST

Proteomic databases

MaxQBiO14232.

Interactioni

Subunit structurei

Component of the TRAMP complex (also called TRF4 complex) composed of at least cid14, mtr4, and air1.1 Publication

Protein-protein interaction databases

BioGridi278135. 17 interactions.
IntActiO14232. 2 interactions.
MINTiMINT-4671612.

Structurei

3D structure databases

ProteinModelPortaliO14232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini207 – 363157Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini441 – 642202Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi311 – 3144DEIH box

Sequence similaritiesi

Belongs to the helicase family. SKI2 subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000163047.
InParanoidiO14232.
KOiK12598.
OMAiEVISPEY.
OrthoDBiEOG092C0MRM.
PhylomeDBiO14232.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR025696. rRNA_proc-arch_dom.
IPR016438. Ski2.
IPR012961. Ski2_C.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF08148. DSHCT. 1 hit.
PF00271. Helicase_C. 1 hit.
PF13234. rRNA_proc-arch. 1 hit.
[Graphical view]
PIRSFiPIRSF005198. Antiviral_helicase_SKI2. 1 hit.
SMARTiSM00487. DEXDc. 1 hit.
SM01142. DSHCT. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14232-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGGELDDAF GVFEGKVPKS LKEESKNSQN SQNSQKIKRT LTDKNASNQE
60 70 80 90 100
QGTKKLESSV GEQESATKRA KIENLKDNQD LIPNNDVNGI HINNSAVADT
110 120 130 140 150
KHKPKIGDIA ADDISNEVSI KNEGDTIPEA TVADSFEQEA SLQVAGKVGM
160 170 180 190 200
TEAKSSTEEV VELRHQVRHQ VSIPPNYDYV PISKHKSPIP PARTYPFTLD
210 220 230 240 250
PFQAVSIACI ERQESVLVSA HTSAGKTVVA EYAVAQSLRD KQRVIYTSPI
260 270 280 290 300
KALSNQKYRE LLAEFGDVGL MTGDVTINPD ATCLVMTTEI LRSMLYRGSE
310 320 330 340 350
VMREVAWVIF DEIHYMRDKE RGVVWEETII LLPDKSHFVF LSATIPNAMQ
360 370 380 390 400
FAEWITKIHR QPCHVVYTDF RPTPLQHYLF PSGSDGIHLV VDEKSNFREE
410 420 430 440 450
NFQRAMSALM EKQGDDPAAM ATKGNAKKGK TGKGGVKGPS DIYKIVKMIM
460 470 480 490 500
VKNYNPVIVF SFSKRECEAL ALQMSKLDMN DQTERDLVTT IFNNAVNQLS
510 520 530 540 550
EKDRELPQIE HILPLLRRGI GIHHSGLLPI LKEVIEILFQ EGLLKVLFAT
560 570 580 590 600
ETFSIGLNMP AKTVVFTNVR KFDGKTFRWI SGGEYIQMSG RAGRRGLDDR
610 620 630 640 650
GIVILMIDEK MDPPVAKSML KGEADRLDSA FHLSYNMILN LLRVEGISPE
660 670 680 690 700
FMLERCFFQF QNSLEVPKLE AKLEESQQHY DSFTILDERP LEEYHTLKTQ
710 720 730 740 750
LERYRTDVRT VVNHPNFCLS FLQGGRLVRV KVGNEDFDWG VVVNVSKRPL
760 770 780 790 800
PKGQSNEYLP QESYIVHTLV MVASDTGPLR IRSGHLPEVH PPAAEDKGKF
810 820 830 840 850
EVVPFLLSSL DGIAHIRVFL PNDLKSQGQK LTVGKALSEV KRRFPEGITL
860 870 880 890 900
LDPVENMNIK EPTFIKLMKK VNILESRLLS NPLHNFSELE EKYAEYLRKL
910 920 930 940 950
ALLEEVKDLK KKLSKARSIM QLDELNSRKR VLRRLGFTTS DDVIEVKGRV
960 970 980 990 1000
ACEISSGDGL LLTELIFNGM FNDLTPEQCA ALLSCLVFQE KSEVENQRMK
1010 1020 1030 1040 1050
EELAGPLKIL QEMARRIAKV SKESKQELNE EEYVNSFKPS LMEVVYAWAH
1060 1070 1080 1090 1100
GASFAQICKM TDVYEGSLIR MFRRLEELIR QMVDAAKVIG NTSLQQKMED
1110
TIACIHRDIV FSASLYL
Length:1,117
Mass (Da):126,191
Last modified:January 1, 1998 - v1
Checksum:iB73209E68247714D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti758 – 7581Y → S in BAA87151 (PubMed:10759889).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11099.1.
AB027847 Genomic DNA. Translation: BAA87151.1.
PIRiT11667.
RefSeqiNP_593302.1. NM_001018732.2.

Genome annotation databases

EnsemblFungiiSPAC6F12.16c.1; SPAC6F12.16c.1:pep; SPAC6F12.16c.
GeneIDi2541639.
KEGGispo:SPAC6F12.16c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11099.1.
AB027847 Genomic DNA. Translation: BAA87151.1.
PIRiT11667.
RefSeqiNP_593302.1. NM_001018732.2.

3D structure databases

ProteinModelPortaliO14232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278135. 17 interactions.
IntActiO14232. 2 interactions.
MINTiMINT-4671612.

Proteomic databases

MaxQBiO14232.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6F12.16c.1; SPAC6F12.16c.1:pep; SPAC6F12.16c.
GeneIDi2541639.
KEGGispo:SPAC6F12.16c.

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.16c.
PomBaseiSPAC6F12.16c. mtr4.

Phylogenomic databases

HOGENOMiHOG000163047.
InParanoidiO14232.
KOiK12598.
OMAiEVISPEY.
OrthoDBiEOG092C0MRM.
PhylomeDBiO14232.

Enzyme and pathway databases

ReactomeiR-SPO-6791226. Major pathway of rRNA processing in the nucleolus.

Miscellaneous databases

PROiO14232.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR025696. rRNA_proc-arch_dom.
IPR016438. Ski2.
IPR012961. Ski2_C.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF08148. DSHCT. 1 hit.
PF00271. Helicase_C. 1 hit.
PF13234. rRNA_proc-arch. 1 hit.
[Graphical view]
PIRSFiPIRSF005198. Antiviral_helicase_SKI2. 1 hit.
SMARTiSM00487. DEXDc. 1 hit.
SM01142. DSHCT. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTR4_SCHPO
AccessioniPrimary (citable) accession number: O14232
Secondary accession number(s): Q9USD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.