Reviewed,
UniProtKB/Swiss-Prot O14230 (FPPS_SCHPO)
Last modified
January 19, 2010.
Version 67.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Farnesyl pyrophosphate synthetase Short name=FPP synthetase Short name=FPS Alternative name(s): Farnesyl diphosphate synthetase Including the following 2 domains: 1- Recommended name: Dimethylallyltranstransferase EC=2.5.1.1 2- Recommended name: Geranyltranstransferase EC=2.5.1.10 | ||||
| Gene names |
| ||||
| Organism | Schizosaccharomyces pombe (Fission yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4896 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 347 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. Ref.4 |
| Catalytic activity | Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate. |
| Cofactor | Binds 3 magnesium ions per subunit By similarity. |
| Pathway | |
| Subunit structure | Interacts with spo9. Ref.4 |
| Subcellular location | |
| Sequence similarities | Belongs to the FPP/GGPP synthetase family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 347 | 347 | Farnesyl pyrophosphate synthetase | PRO_0000123951 | |||||
Sites | |||||||||
| Metal binding | 95 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 95 | 1 | Magnesium 2 By similarity | ||||||
| Metal binding | 99 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 99 | 1 | Magnesium 2 By similarity | ||||||
| Metal binding | 235 | 1 | Magnesium 3 By similarity | ||||||
| Binding site | 50 | 1 | Isopentenyl diphosphate By similarity | ||||||
| Binding site | 53 | 1 | Isopentenyl diphosphate By similarity | ||||||
| Binding site | 88 | 1 | Isopentenyl diphosphate By similarity | ||||||
| Binding site | 104 | 1 | Dimethylallyl diphosphate By similarity | ||||||
| Binding site | 105 | 1 | Isopentenyl diphosphate By similarity | ||||||
| Binding site | 192 | 1 | Dimethylallyl diphosphate By similarity | ||||||
| Binding site | 193 | 1 | Dimethylallyl diphosphate By similarity | ||||||
| Binding site | 232 | 1 | Dimethylallyl diphosphate By similarity | ||||||
| Binding site | 249 | 1 | Dimethylallyl diphosphate By similarity | ||||||
| Binding site | 258 | 1 | Dimethylallyl diphosphate By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 90 | 1 | F → C: Complements lethality of fps1-delete strain. Ref.4 | ||||||
| Mutagenesis | 104 | 1 | R → Q: No affect on GGPP synthetase activity. Ref.4 | ||||||
| Sequence conflict | 203 | 1 | A → E in BAA13767. Ref.2 | ||||||
| Sequence conflict | 346 – 347 | 2 | NK → ISKFLVLCF in BAA13767. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972. |
| [2] | "Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-347. Strain: PR745. |
| [3] | "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [4] | "Geranylgeranyl diphosphate synthase in fission yeast is a heteromer of farnesyl diphosphate synthase (FPS), Fps1, and an FPS-like protein, Spo9, essential for sporulation." Ye Y., Fujii M., Hirata A., Kawamukai M., Shimoda C., Nakamura T. Mol. Biol. Cell 18:3568-3581(2007) [PubMed: 17596513] [Abstract] Cited for: FUNCTION, INTERACTION WITH SPO9, MUTAGENESIS OF PHE-90 AND ARG-104. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CU329670 Genomic DNA. Translation: CAB11097.1. D89104 mRNA. Translation: BAA13767.1. |
| PIR | T11664. T42081. |
| RefSeq | NP_593299.1. |
3D structure databases | |
| SMR | O14230. Positions 5-347. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O14230. |
Genome annotation databases | |
| GeneID | 2542898. |
| GenomeReviews | Gene locus fps1 in contig CU329670_GR. |
| KEGG | spo:SPAC6F12.13c. |
| NMPDR | fig|4896.1.peg.3269. |
Organism-specific databases | |
| GeneDB_Spombe | SPAC6F12.13c. |
Phylogenomic databases | |
| eggNOG | fuNOG05213. |
| HOGENOM | HBG328346. |
| OMA | ELFHEVT. |
| OrthoDB | EOG9D286Q. |
| PhylomeDB | O14230. |
Enzyme and pathway databases | |
| BRENDA | 2.5.1.1. 653. 2.5.1.10. 653. |
Gene expression databases | |
| ArrayExpress | O14230. |
Family and domain databases | |
| InterPro | IPR000092. Polyprenyl_synt. IPR008949. Terpenoid_synth. [Graphical view] |
| Gene3D | G3DSA:1.10.600.10. Terpenoid_synth. 1 hit. |
| Pfam | PF00348. polyprenyl_synt. 1 hit. [Graphical view] |
| PROSITE | PS00723. POLYPRENYL_SYNTHET_1. 1 hit. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FPPS_SCHPO | ||||||||
| Accession | Primary (citable) accession number: O14230 Secondary accession number(s): P78756 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


