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Protein

Probable phosphoribosylformylglycinamidine synthase

Gene

ade3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (By similarity).By similarity

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable phosphoribosylformylglycinamidine synthase (ade3)
  2. Bifunctional purine biosynthetic protein ADE1 (ade1)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei691ATP; via carbonyl oxygenBy similarity1
Metal bindingi692MagnesiumBy similarity1
Metal bindingi733MagnesiumBy similarity1
Metal bindingi737MagnesiumBy similarity1
Metal bindingi903MagnesiumBy similarity1
Binding sitei905ATPBy similarity1
Active sitei1156NucleophileBy similarity1
Active sitei1284By similarity1
Active sitei1286By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi312 – 323ATPSequence analysisAdd BLAST12
Nucleotide bindingi391 – 393ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphoribosylformylglycinamidine synthase (EC:6.3.5.3)
Short name:
FGAM synthase
Short name:
FGAMS
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase
Short name:
FGAR amidotransferase
Short name:
FGAR-AT
Formylglycinamide ribotide amidotransferase
Gene namesi
Name:ade3
Synonyms:min11
ORF Names:SPAC6F12.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.10c.
PomBaseiSPAC6F12.10c. ade3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • Golgi apparatus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001004041 – 1323Probable phosphoribosylformylglycinamidine synthaseAdd BLAST1323

Proteomic databases

MaxQBiO14228.
PRIDEiO14228.

Interactioni

Protein-protein interaction databases

BioGridi279316. 7 interactors.
MINTiMINT-4671580.

Structurei

3D structure databases

ProteinModelPortaliO14228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1062 – 1306Glutamine amidotransferase type-1Add BLAST245

Sequence similaritiesi

In the N-terminal section; belongs to the FGAMS family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000261359.
InParanoidiO14228.
KOiK01952.
OMAiKAETHNF.
OrthoDBiEOG092C08F5.
PhylomeDBiO14228.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVYYGGSAL SVQSKKKILE LTVSGVSDLN AVYFYLIYTK SNSSLNCESL
60 70 80 90 100
RPILSDLQES EFKPDGTTMV YVFPRPGTIS PWSSKATNIA NVCGYKDVIR
110 120 130 140 150
IERGIAYSVV FKDDISEEML KSALNHLYDR MTEALRFKLP EEDEVFDKHE
160 170 180 190 200
PAPLVRIELN CGQGGDKQAA TERLNHANKK FGLALAPDEI DYLVECYTSE
210 220 230 240 250
PSLKSREPTD VELFMFGQVN SEHCRHKIFN ADWTIDGEKK DYSLFKMIRN
260 270 280 290 300
THLKNPQYTI SAYSDNAAVF EGNSGTLFAP VNGIWSMKDE PVEFLGKVET
310 320 330 340 350
HNHPTAVSPF PGAATGSGGE IRDEGAVGQG SLSKAGLAGY SVSDLNIPGY
360 370 380 390 400
KQPWELDVGK PYHIATSLDI MLEAPIGSSA FNNEFGRPCI NGYFRTFCME
410 420 430 440 450
VPRGDGTLEI RGYHKPIMAA GGIGRIRKQH AFKKSIAPGS PIIVLGGPAL
460 470 480 490 500
LVGLGGGAAS SMNAGEGSEE LDFASVQRGN PEMQRRAQMV IDACTTMDEN
510 520 530 540 550
IIQSIHDVGA GGVSNALPEL VHDAGLGARF ELRDIPCIEP SMSPMQIWCC
560 570 580 590 600
ESQERYVLSV KSEDLDTFKS ICERERCPYG VVGYSTVEQR LILTDRLYNT
610 620 630 640 650
TPIDLPMEVL FGKPPKMSRV AETQTIPLSK FDSSLKSYLA PSSDPILDAV
660 670 680 690 700
ERVLRMPAVA SKSFLITIGD RSVTGLIARD QMVGPWQVPV ADVGVTVTSY
710 720 730 740 750
GKGINTGEAL AMGEKPISAL VSAAASARMA VAECIMNLVA ASIPALDRIR
760 770 780 790 800
LSANWMAAPS HPGEGAKLYE AVQAIGLELC PSLGISIPVG KDSMSMSMKW
810 820 830 840 850
NEDGREKSVT APLSLIITGF SPVDDLYSIW TPQLRKVEDI GSTSLIFIDL
860 870 880 890 900
ANGKQRLGGS ILAQSYKQLG DEVPDLDNLD TFKNFINVIT QLHKTNYIQA
910 920 930 940 950
YHDKSDGGLF VTLSEMAFAG HVGIECELDS LSSDNIAALF NEELGAVIQV
960 970 980 990 1000
CDRDIAKVLE LFAANGLSTC VHRIGKVLSG QAQTISFSRS GKIIFKSTRS
1010 1020 1030 1040 1050
KLHGIWHETS YKMQEIRDNP ECARQEMENI ADNNDPGLGY HLTFDPNVSV
1060 1070 1080 1090 1100
TADLALTSRP KVAILREQGV NGYLEMAYAF YASGFTAVDV HMTDILSGRV
1110 1120 1130 1140 1150
HLDDFVGIAA CGGFSYGDVL GSGNGWATSI LLHEDARNEF YRFFNERKDT
1160 1170 1180 1190 1200
FGLGICNGCQ LFSRLKSLIP GAKSWPMFTF NESAQYEGRA VMLKIDETSG
1210 1220 1230 1240 1250
SKSIFTESMA GSSLPVVVAH GEGRAVFDSE SDYEQFKKEG LDLIYYVNNY
1260 1270 1280 1290 1300
NERTSRYPFN PNGSRDAIAG VRSPCGRFLA MMPHPERVVL KVANSYYPHS
1310 1320
KASEWGVHGP WIRLFQSARK WVG
Length:1,323
Mass (Da):144,867
Last modified:January 1, 1998 - v1
Checksum:i7D31A6BEFAE2997F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11094.1.
AB027799 Genomic DNA. Translation: BAA87103.1.
PIRiT11661.
RefSeqiNP_593296.1. NM_001018726.2.

Genome annotation databases

EnsemblFungiiSPAC6F12.10c.1; SPAC6F12.10c.1:pep; SPAC6F12.10c.
GeneIDi2542871.
KEGGispo:SPAC6F12.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11094.1.
AB027799 Genomic DNA. Translation: BAA87103.1.
PIRiT11661.
RefSeqiNP_593296.1. NM_001018726.2.

3D structure databases

ProteinModelPortaliO14228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279316. 7 interactors.
MINTiMINT-4671580.

Proteomic databases

MaxQBiO14228.
PRIDEiO14228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6F12.10c.1; SPAC6F12.10c.1:pep; SPAC6F12.10c.
GeneIDi2542871.
KEGGispo:SPAC6F12.10c.

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.10c.
PomBaseiSPAC6F12.10c. ade3.

Phylogenomic databases

HOGENOMiHOG000261359.
InParanoidiO14228.
KOiK01952.
OMAiKAETHNF.
OrthoDBiEOG092C08F5.
PhylomeDBiO14228.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.
ReactomeiR-SPO-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiO14228.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR4_SCHPO
AccessioniPrimary (citable) accession number: O14228
Secondary accession number(s): Q9USF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.