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O14228 (PUR4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:ade3
Synonyms:min11
ORF Names:SPAC6F12.10c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length1323 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentGolgi apparatus

Inferred from direct assay. Source: GeneDB_Spombe

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13231323Probable phosphoribosylformylglycinamidine synthase
PRO_0000100404

Regions

Domain1062 – 1306245Glutamine amidotransferase type-1
Nucleotide binding312 – 32312ATP Potential

Sites

Active site11561For GATase activity By similarity

Sequences

Sequence LengthMass (Da)Tools
O14228 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 7D31A6BEFAE2997F

FASTA1,323144,867
        10         20         30         40         50         60 
MLVYYGGSAL SVQSKKKILE LTVSGVSDLN AVYFYLIYTK SNSSLNCESL RPILSDLQES 

        70         80         90        100        110        120 
EFKPDGTTMV YVFPRPGTIS PWSSKATNIA NVCGYKDVIR IERGIAYSVV FKDDISEEML 

       130        140        150        160        170        180 
KSALNHLYDR MTEALRFKLP EEDEVFDKHE PAPLVRIELN CGQGGDKQAA TERLNHANKK 

       190        200        210        220        230        240 
FGLALAPDEI DYLVECYTSE PSLKSREPTD VELFMFGQVN SEHCRHKIFN ADWTIDGEKK 

       250        260        270        280        290        300 
DYSLFKMIRN THLKNPQYTI SAYSDNAAVF EGNSGTLFAP VNGIWSMKDE PVEFLGKVET 

       310        320        330        340        350        360 
HNHPTAVSPF PGAATGSGGE IRDEGAVGQG SLSKAGLAGY SVSDLNIPGY KQPWELDVGK 

       370        380        390        400        410        420 
PYHIATSLDI MLEAPIGSSA FNNEFGRPCI NGYFRTFCME VPRGDGTLEI RGYHKPIMAA 

       430        440        450        460        470        480 
GGIGRIRKQH AFKKSIAPGS PIIVLGGPAL LVGLGGGAAS SMNAGEGSEE LDFASVQRGN 

       490        500        510        520        530        540 
PEMQRRAQMV IDACTTMDEN IIQSIHDVGA GGVSNALPEL VHDAGLGARF ELRDIPCIEP 

       550        560        570        580        590        600 
SMSPMQIWCC ESQERYVLSV KSEDLDTFKS ICERERCPYG VVGYSTVEQR LILTDRLYNT 

       610        620        630        640        650        660 
TPIDLPMEVL FGKPPKMSRV AETQTIPLSK FDSSLKSYLA PSSDPILDAV ERVLRMPAVA 

       670        680        690        700        710        720 
SKSFLITIGD RSVTGLIARD QMVGPWQVPV ADVGVTVTSY GKGINTGEAL AMGEKPISAL 

       730        740        750        760        770        780 
VSAAASARMA VAECIMNLVA ASIPALDRIR LSANWMAAPS HPGEGAKLYE AVQAIGLELC 

       790        800        810        820        830        840 
PSLGISIPVG KDSMSMSMKW NEDGREKSVT APLSLIITGF SPVDDLYSIW TPQLRKVEDI 

       850        860        870        880        890        900 
GSTSLIFIDL ANGKQRLGGS ILAQSYKQLG DEVPDLDNLD TFKNFINVIT QLHKTNYIQA 

       910        920        930        940        950        960 
YHDKSDGGLF VTLSEMAFAG HVGIECELDS LSSDNIAALF NEELGAVIQV CDRDIAKVLE 

       970        980        990       1000       1010       1020 
LFAANGLSTC VHRIGKVLSG QAQTISFSRS GKIIFKSTRS KLHGIWHETS YKMQEIRDNP 

      1030       1040       1050       1060       1070       1080 
ECARQEMENI ADNNDPGLGY HLTFDPNVSV TADLALTSRP KVAILREQGV NGYLEMAYAF 

      1090       1100       1110       1120       1130       1140 
YASGFTAVDV HMTDILSGRV HLDDFVGIAA CGGFSYGDVL GSGNGWATSI LLHEDARNEF 

      1150       1160       1170       1180       1190       1200 
YRFFNERKDT FGLGICNGCQ LFSRLKSLIP GAKSWPMFTF NESAQYEGRA VMLKIDETSG 

      1210       1220       1230       1240       1250       1260 
SKSIFTESMA GSSLPVVVAH GEGRAVFDSE SDYEQFKKEG LDLIYYVNNY NERTSRYPFN 

      1270       1280       1290       1300       1310       1320 
PNGSRDAIAG VRSPCGRFLA MMPHPERVVL KVANSYYPHS KASEWGVHGP WIRLFQSARK 


WVG

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329670 Genomic DNA. Translation: CAB11094.1.
PIRT11661.
RefSeqNP_593296.1. NM_001018726.1.

3D structure databases

ProteinModelPortalO14228.
ModBaseSearch...

Protein-protein interaction databases

STRINGO14228.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC6F12.10c.1; SPAC6F12.10c.1:pep; SPAC6F12.10c.
GeneID2542871.
GenomeReviewsGene locus ade3 in contig CU329670_GR.
KEGGspo:SPAC6F12.10c.
NMPDRfig|4896.1.peg.3266.

Organism-specific databases

GeneDB_SpombeSPAC6F12.10c.

Phylogenomic databases

eggNOGfuNOG05037.
GeneTreeEFGT00050000004928.
HOGENOMHBG335309.
OMAASASAKM.
OrthoDBEOG4V72QQ.

Enzyme and pathway databases

BioCycSPOM-XXX-01:SPOM-XXX-01-000995-MONOMER.

Gene expression databases

ArrayExpressO14228.

Family and domain databases

InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_SCHPO
AccessionPrimary (citable) accession number: O14228
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: December 14, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents