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Protein

RNA-dependent RNA polymerase 1

Gene

rdp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation, accurate chromosome segregation, centromere cohesion and telomere function during mitosis and meiosis. Required for both post-transcriptional and transcriptional gene silencing. Required for silencing at the centromeres and for initiation of transcriptionally silent heterochromatin at the mating type locus. Promotes histone H3 'Lys-10' methylation necessary for centromere function. Required for recruitment of swi6 and cohesin to an ectopic dg repeat. A member of the RNA-directed RNA polymerase complex (RDRC) which is involved in the generation of small interfering RNAs (siRNAs) and mediates their association with the RNA-induced transcriptional silencing (RITS) complex. RITS acts as a priming complex for dsRNA synthesis at the site of non-coding centromeric RNA. Its RNA-dependent RNA polymerase activity is critical in siRNA production necessary for heterochromatin formation.8 Publications

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

  • RNA binding Source: UniProtKB-KW
  • RNA-directed RNA polymerase activity Source: PomBase

GO - Biological processi

  • cellular protein localization Source: PomBase
  • chromatin silencing at centromere Source: PomBase
  • chromatin silencing at centromere outer repeat region Source: PomBase
  • chromatin silencing by small RNA Source: PomBase
  • co-transcriptional gene silencing by RNA interference machinery Source: PomBase
  • gene silencing by RNA Source: PomBase
  • meiotic telomere clustering Source: PomBase
  • production of siRNA involved in chromatin silencing by small RNA Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Chromosome partition, RNA-mediated gene silencing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BRENDAi2.7.7.48. 5613.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-dependent RNA polymerase 1 (EC:2.7.7.48)
Short name:
Protein rdp1
Gene namesi
Name:rdp1
Synonyms:csp7, rdr1
ORF Names:SPAC6F12.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.09.
PomBaseiSPAC6F12.09. rdp1.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric outer repeat region Source: GOC
  • chromosome, telomeric region Source: UniProtKB-SubCell
  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • mating-type region heterochromatin Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear pericentric heterochromatin Source: PomBase
  • nuclear RNA-directed RNA polymerase complex Source: PomBase
  • nuclear subtelomeric heterochromatin Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Nucleus, Telomere

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi903 – 9031D → A: Abolishes RNA-dependent RNA polymerase activity and results in loss of transcriptional silencing and heterochromatin formation at centromeres together with defects in mitotic chromosome segregation and telomere clustering. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12151215RNA-dependent RNA polymerase 1PRO_0000097211Add
BLAST

Interactioni

Subunit structurei

Cid12, hrr1 and rdp1 interact forming the RNA-directed RNA polymerase complex (RDRC). The RDRC complex interacts with the RITS complex via interaction between ago1 and hrr1. Clr4 has a role in mediating this interaction.

Protein-protein interaction databases

BioGridi278673. 78 interactions.
DIPiDIP-57038N.
IntActiO14227. 1 interaction.
MINTiMINT-4671558.

Family & Domainsi

Sequence similaritiesi

Belongs to the RdRP family.Curated

Phylogenomic databases

InParanoidiO14227.
KOiK11699.
OMAiYDERIVP.
OrthoDBiEOG092C0LSZ.
PhylomeDBiO14227.

Family and domain databases

InterProiIPR007855. RNA-dep_RNA_pol_euk-typ.
[Graphical view]
PANTHERiPTHR23079. PTHR23079. 1 hit.
PfamiPF05183. RdRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14227-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSLNDFIS VKLKRYSRES PWERLRVPYR NKKQKKWASV HNNEAQLHSA
60 70 80 90 100
NKRNDNCLIQ RSSTWRLGDM ITLVIKDIPV TWLSNEGGKL YNLWEPLHDY
110 120 130 140 150
GTIEFMKINE PLNGQTSTTA IVQFAPPPKV PFWEPNGKIN VKGVDLAVQI
160 170 180 190 200
DITAHRSHIS RQVFSKNSFR SDQLVKIPLS SFKLGQVYDE RIVPLFGVDC
210 220 230 240 250
GITVTESNLL VYFNFKKLCV LFDASFDKQI ETFRLDFDFH SIIGDVGTDY
260 270 280 290 300
YDDHISLVFR FRFSPLIFRK SKNATESRVQ TFWTASHLWR RHYDILPFNV
310 320 330 340 350
SPTTASPIEL LNCHNAPIGR CNVLVLSFSI RDESDKDDIA FLLHNLEKFN
360 370 380 390 400
LKSQLDKVVF HLVPDYKHRC SLINDKEIEE EIAYLLQACL SKNLLSEIDL
410 420 430 440 450
PIILANLKKL SKERAKKFLR LILTSKTALI NPSELDFTKS FVFYDLSSAS
460 470 480 490 500
SIHIKKLYVT PTTLRIVEDS LEAGNRVIRN FKDFANRFMR VQITDEYYKQ
510 520 530 540 550
KIRGGSDGFR NEKLYSRIQQ LLTYGIKVGN QIYEFLAFGN SQLREHGAYF
560 570 580 590 600
FASGSDLNAK QIREWMGDFS EINSVSKYAA RMGQCFSTTK EINRFCVDIS
610 620 630 640 650
LQDDIVRNNH CFTDGVGMAS LSVIRRLSLE VKNHDMFPSA FQFRMGGYKG
660 670 680 690 700
VLSLAPPTKL EYHQGNLVFP RRSQDKFKSF HSTLEVIKIS RFSNAHLNMQ
710 720 730 740 750
LITLLEGLGV EKTVFLELTR SQLSKMNESI NSKQKSILML RDNVDEYHST
760 770 780 790 800
LIIADFIQAG FLERDDAFTE NLLNLYYEWV LRLIKEKQKV SVPKGAYLLG
810 820 830 840 850
VADETGTLKG HYDDAVLSVP EIFIQITDTS TSFGSYSTGK LKTRVIVGLC
860 870 880 890 900
IVARNPSLHP GDVRVCKAVR CDELMHLKNV IVFPTTGDRS IPAMCSGGDL
910 920 930 940 950
DGDEYTVIWD QRLLPKIVNY PPLLESSPKK SIDFLEGKPL IDSVKEFFVN
960 970 980 990 1000
YIKYDSLGLI SNAWKAWAHD HDNNPEGIFG NVCLELAEMH SKAVDFAKSG
1010 1020 1030 1040 1050
VACKMQAKYH PKRYPDFMQK TKTRSFRSET AVGKIFRYAA RFQRESGRPA
1060 1070 1080 1090 1100
TYNPIMNTVY DPCMKLPRFK TEYLNVAEEV KKHYDNDLRS IMARFDISTE
1110 1120 1130 1140 1150
YEVYTAFILF KDDLAKTVNE YGLREEVSFQ FDLLKKKYTQ EYLEKCALSN
1160 1170 1180 1190 1200
QSAFDSSEYE ERINSAVAAT YDVTYDQRVK SVGNGTTEVL ISFPYLFSSR
1210
LCQLSRKAML TANNF
Length:1,215
Mass (Da):139,478
Last modified:January 1, 1998 - v1
Checksum:i389B95C8217CB05C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11093.1.
PIRiT11660.
RefSeqiNP_593295.1. NM_001018725.2.

Genome annotation databases

EnsemblFungiiSPAC6F12.09.1; SPAC6F12.09.1:pep; SPAC6F12.09.
GeneIDi2542198.
KEGGispo:SPAC6F12.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11093.1.
PIRiT11660.
RefSeqiNP_593295.1. NM_001018725.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278673. 78 interactions.
DIPiDIP-57038N.
IntActiO14227. 1 interaction.
MINTiMINT-4671558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6F12.09.1; SPAC6F12.09.1:pep; SPAC6F12.09.
GeneIDi2542198.
KEGGispo:SPAC6F12.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC6F12.09.
PomBaseiSPAC6F12.09. rdp1.

Phylogenomic databases

InParanoidiO14227.
KOiK11699.
OMAiYDERIVP.
OrthoDBiEOG092C0LSZ.
PhylomeDBiO14227.

Enzyme and pathway databases

BRENDAi2.7.7.48. 5613.

Miscellaneous databases

PROiO14227.

Family and domain databases

InterProiIPR007855. RNA-dep_RNA_pol_euk-typ.
[Graphical view]
PANTHERiPTHR23079. PTHR23079. 1 hit.
PfamiPF05183. RdRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRDP1_SCHPO
AccessioniPrimary (citable) accession number: O14227
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.