O14215 (PRI1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase small subunit
EC=2.7.7.-

Gene names

Name:	pri1
Synonyms:	spp1
ORF Names:	SPAC6B12.10c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.
Subunit structure	Heterodimer of a small subunit and a large subunit.
Miscellaneous	The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA By similarity.
Sequence similarities	Belongs to the eukaryotic-type primase small subunit family.

Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	telomere maintenance Inferred from mutant phenotype PubMed 12697806. Source: PomBase
Cellular_component	alpha DNA polymerase:primase complex Traceable author statement Ref.2. Source: PomBase nuclear chromatin Inferred by curator. Source: PomBase
Molecular_function	DNA primase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pri2	O74761	2	EBI-849063,EBI-849075

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	DNA primase small subunit		PRO_0000046735
Regions
	Motif	143 – 153	11	Zinc knuckle motif
Sites
	Active site	66	1	Potential
	Active site	131	1	Potential
	Active site	133	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O14215 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 71526FFAF7C2BBFA	FASTA	454	52,009
	10 20 30 40 50 60 MTVQIDELDD KDLDEIIANG TLDGAKQGAV DSETMIQYYR HLFPWKYLFQ WLNHGPVVTN 70 80 90 100 110 120 DFAHREFAFT LPNDAYIRYL SFSNWEELKK EALNLCPSRF EVGPVYSANP RDRKTIRKST 130 140 150 160 170 180 FHPLKKELVF DIDMTDYDDV RTCCSKTNIC EKCWPFITIA VQVLDICFHE DFGFKHILWV 190 200 210 220 230 240 YSGRRGIHAW ICDEIACSLD DRSRRMIASY LQVVVGNPQG GVRLINNLKR PLHPHLTRSL 250 260 270 280 290 300 NILKSAFVKI VLEDQDPWAS KEGAENLLKL LPDKDLASAL RKKWEVDPER SSKNKWSDID 310 320 330 340 350 360 TVLASGSIAS ISPSVIAIAK QDIVLTYLYP RLDVEVSRHL NHLLKSPFCV HPGTSRVCVP 370 380 390 400 410 420 IDIERMDSFN PLKVPTVNDL LQELDKNSQN DNGHGPTMET NTTENQKDNA RGQSNKGHGF 430 440 450 STSLNPYTLY FKSFSSQLFK ETVGNKRKHE NLEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation." Tan S., Wang T.S. Mol. Cell. Biol. 20:7853-7866(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB11078.1.
PIR	T39017.
RefSeq	NP_593765.1. NM_001019195.2.
3D structure databases
ProteinModelPortal	O14215.
ModBase	Search...
Protein-protein interaction databases
IntAct	O14215. 4 interactions.
MINT	MINT-4671447.
STRING	4896.SPAC6B12.10c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC6B12.10c.1; SPAC6B12.10c.1:pep; SPAC6B12.10c.
GeneID	2543323.
KEGG	spo:SPAC6B12.10c.
Organism-specific databases
PomBase	SPAC6B12.10c.
Phylogenomic databases
eggNOG	COG1467.
HOGENOM	HOG000196041.
KO	K02684.
OMA	GRRGAHC.
OrthoDB	EOG4CVKGS.
Family and domain databases
InterPro	IPR002755. DNA_primase_S. IPR014052. DNA_primase_ssu_euk/arc. [Graphical view]
PANTHER	PTHR10536. PTHR10536. 1 hit.
Pfam	PF01896. DNA_primase_S. 1 hit. [Graphical view]
TIGRFAMs	TIGR00335. primase_sml. 1 hit.
ProtoNet	Search...
Other
NextBio	20804339.

Entry information

Entry name

PRI1_SCHPO

Accession

Primary (citable) accession number: O14215

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 29, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program