O14214 (TRM10_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(9)-N1)-methyltransferase
EC=2.1.1.221

Alternative name(s):
tRNA methyltransferase 10
tRNA(m1G9)-methyltransferase
Short name=tRNA(m1G9)MTase

Gene names

Name:	trm10
ORF Names:	SPAC6B12.09

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine9 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine9 in tRNA.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA methylation Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: GeneDB_Spombe nucleolus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	tRNA (guanine(9)-N1)-methyltransferase		PRO_0000060518
Amino acid modifications
	Modified residue	296	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O14214 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C41B7EE19741BFC8	FASTA	304	35,470
	10 20 30 40 50 60 MENKDALDIG KDDTNTSEAD VSKNETQEQP VLSKSALKRL KRQQEWDAGR EKRAEMRREK 70 80 90 100 110 120 KRLRKEERKR KIEAGEVVKS QKKRIRLGKV VPSSIRIVLD CAFDDLMNDK EINSLCQQVT 130 140 150 160 170 180 RCHSANRTAL HPVELFATNF GGRLKTRQDF VLKGQQNNWK RYNPTTKSYL EEFESQKEKL 190 200 210 220 230 240 VYLSADSDNT ITELDEDKIY IIGAIVDKNR YKNLCQNKAS EQGIKTAKLP IDEYIKITDR 250 260 270 280 290 300 KILTVNQVFE ILSLWLEYRD WEKAFMEVIP KRKGILLKSD ESFDVSEDTR SQSNQSDSEL EKEN

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-296, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB11070.1.
PIR	T39016.
RefSeq	NP_593764.1. NM_001019194.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O14214.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC6B12.09.1; SPAC6B12.09.1:pep; SPAC6B12.09.
GeneID	2543319.
GenomeReviews	Gene locus trm10 in contig CU329670_GR.
KEGG	spo:SPAC6B12.09.
NMPDR	fig|4896.1.peg.3734.
Organism-specific databases
GeneDB_Spombe	SPAC6B12.09.
Phylogenomic databases
eggNOG	fuNOG04600.
GeneTree	EFGT00050000003459.
HOGENOM	HBG717479.
OMA	SNVERKQ.
OrthoDB	EOG48D43Z.
Gene expression databases
ArrayExpress	O14214.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. IPR016653. tRNA_m1G_mtfrase_met. [Graphical view]
KO	K15445.
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF016323. tRNA_m1G_mtfrase_met. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM10_SCHPO

Accession

Primary (citable) accession number: O14214

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program