O14199 (ALG14_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine transferase subunit alg14

Alternative name(s):
Asparagine-linked glycosylation protein 14

Gene names

Name:	alg14
ORF Names:	SPAC5D6.06c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit alg13 to the ER By similarity.
Subunit structure	Heterodimer with alg13 to form a functional enzyme By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG14 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	dolichol-linked oligosaccharide biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	integral to endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	UDP-N-acetylglucosamine transferase subunit alg14		PRO_0000123816
Regions
	Topological domain	1 – 3	3	Lumenal Potential
	Transmembrane	4 – 21	18	Helical; Potential
	Topological domain	22 – 210	189	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	O14199 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F29F81163B27963C	FASTA	210	23,210
	10 20 30 40 50 60 MNTYVLTAIA VLASLIILLV GRNAIKSSKK KPFQKHLLVF FGSGGHTGEM LNLLNALDDK 70 80 90 100 110 120 LYSVRSYVAG SDDTMSVSKA SLLSNSLPSV KSKIFKVPRA RYVKQSWLTT PFTAFWSLLG 130 140 150 160 170 180 SISVIFWNPF GIPDVILCNG PGTCVFICLL GYLAKFLGKN VKIVYVESFA RVKSLSLSGK 190 200 210 ILMPFVDRFL VQWPDLATKY KRAEYIGIVA

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB10854.1.
PIR	T38960.
RefSeq	NP_593363.1. NM_001018795.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O14199.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC5D6.06c.1; SPAC5D6.06c.1:pep; SPAC5D6.06c.
GeneID	2541499.
GenomeReviews	Gene locus alg14 in contig CU329670_GR.
KEGG	spo:SPAC5D6.06c.
NMPDR	fig|4896.1.peg.3333.
Organism-specific databases
GeneDB_Spombe	SPAC5D6.06c.
Phylogenomic databases
eggNOG	fuNOG11503.
GeneTree	EFGT00050000006268.
HOGENOM	HBG735599.
OMA	WISSIFT.
OrthoDB	EOG4NGKX9.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001056-MONOMER.
Gene expression databases
ArrayExpress	O14199.
Family and domain databases
InterPro	IPR013969. Oligosacch_biosynth_Alg14. [Graphical view]
KO	K07441.
PANTHER	PTHR12154. Oligosacch_biosynth_Alg14. 1 hit.
Pfam	PF08660. Alg14. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG14_SCHPO

Accession

Primary (citable) accession number: O14199

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program