O14190 (ALG13_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine transferase subunit alg13
EC=2.4.1.141

Alternative name(s):
Asparagine-linked glycosylation protein 13

Gene names

Name:	alg13
ORF Names:	SPAC56E4.02c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-diphosphodolichol = UDP + N,N'-diacetylchitobiosyl-diphosphodolichol.
Subunit structure	Heterodimer with alg14 to form a functional enzyme By similarity.
Subcellular location	Endoplasmic reticulum By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dolichol-linked oligosaccharide biosynthetic process Inferred from sequence orthology PubMed 15615718. Source: PomBase lipid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase endoplasmic reticulum Inferred from sequence orthology. Source: PomBase nucleus Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity Inferred from sequence orthology. Source: PomBase carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	UDP-N-acetylglucosamine transferase subunit alg13		PRO_0000215603

Sequences

	Sequence		Length	Mass (Da)
	O14190 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E93A6A93FF52CB35	FASTA	162	17,769
	10 20 30 40 50 60 MNAFVTVGST QFDDLIRAVL KPEFQHCLVK HGINQLIVQY GKGKQAFGDP KSVAGLTILG 70 80 90 100 110 120 FDYAPEIESY IHDASIVISH AGAGSILQTL RSGKRLLVVP NESLMDNHQV ELATKLASMN 130 140 150 160 YLVTCSTSNL VEGLEELYPK ILTPFPKSDC STFQKVMQDV AR

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB16393.1.
PIR	T38904.
RefSeq	NP_593269.1. NM_001018666.2.
3D structure databases
ProteinModelPortal	O14190.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC56E4.02c-1.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC56E4.02c.1; SPAC56E4.02c.1:pep; SPAC56E4.02c.
GeneID	2543352.
KEGG	spo:SPAC56E4.02c.
Organism-specific databases
PomBase	SPAC56E4.02c.
Phylogenomic databases
eggNOG	COG5017.
HOGENOM	HOG000192807.
KO	K07432.
OMA	SHLLYCT.
OrthoDB	EOG4KM2CB.
Family and domain databases
InterPro	IPR007235. Glyco_trans_28_C. [Graphical view]
Pfam	PF04101. Glyco_tran_28_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804367.

Entry information

Entry name

ALG13_SCHPO

Accession

Primary (citable) accession number: O14190

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program