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Protein

Ras GTPase-activating-like protein rng2

Gene

rng2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for cytokinesis. Component of the contractile F-actin ring; required for its construction following assembly of F-actin at the division site.1 Publication

GO - Molecular functioni

  • actin binding Source: PomBase
  • calmodulin binding Source: UniProtKB

GO - Biological processi

  • actin filament bundle convergence involved in mitotic contractile ring assembly Source: PomBase
  • barrier septum assembly Source: UniProtKB-KW
  • mitotic actomyosin contractile ring assembly Source: PomBase
  • mitotic actomyosin contractile ring assembly actin filament organization Source: PomBase
  • mitotic actomyosin contractile ring localization Source: PomBase
  • mitotic contractile ring actin filament bundle assembly Source: PomBase
  • mitotic cytokinesis Source: PomBase
  • protein localization to medial cortex Source: PomBase
  • regulation of GTPase activity Source: InterPro
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Septation

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-SPO-5626467. RHO GTPases activate IQGAPs.
R-SPO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating-like protein rng2
Alternative name(s):
Ring assembly protein 2
Gene namesi
Name:rng2
ORF Names:SPAC4F8.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4F8.13c.
PomBaseiSPAC4F8.13c. rng2.

Subcellular locationi

GO - Cellular componenti

  • actomyosin contractile ring Source: PomBase
  • cytoplasm Source: PomBase
  • medial cortical node Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nuclear envelope Source: UniProtKB-SubCell
  • spindle pole body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566651 – 1489Ras GTPase-activating-like protein rng2Add BLAST1489

Proteomic databases

MaxQBiO14188.
PRIDEiO14188.

Interactioni

Subunit structurei

Interacts with calmodulin (cam1).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
act1P109893EBI-1152490,EBI-617028
cam1P059332EBI-1152490,EBI-1152513

GO - Molecular functioni

  • actin binding Source: PomBase
  • calmodulin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi279561. 44 interactors.
IntActiO14188. 2 interactors.
MINTiMINT-4671212.

Structurei

Secondary structure

11489
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 55Combined sources23
Helixi62 – 64Combined sources3
Helixi65 – 68Combined sources4
Turni69 – 71Combined sources3
Helixi73 – 82Combined sources10
Beta strandi94 – 96Combined sources3
Helixi100 – 113Combined sources14
Helixi118 – 120Combined sources3
Helixi124 – 127Combined sources4
Helixi133 – 148Combined sources16
Turni149 – 151Combined sources3
Helixi167 – 179Combined sources13
Helixi187 – 189Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P2XX-ray2.21A32-190[»]
1P5SX-ray2.22A1-190[»]
ProteinModelPortaliO14188.
SMRiO14188.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO14188.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 147CHPROSITE-ProRule annotationCuratedAdd BLAST107
Domaini359 – 388IQ 1PROSITE-ProRule annotationCuratedAdd BLAST30
Domaini389 – 418IQ 2PROSITE-ProRule annotationCuratedAdd BLAST30
Domaini418 – 449IQ 3PROSITE-ProRule annotationCuratedAdd BLAST32
Domaini535 – 564IQ 4PROSITE-ProRule annotationCuratedAdd BLAST30
Domaini565 – 594IQ 5PROSITE-ProRule annotationCuratedAdd BLAST30
Domaini655 – 684IQ 6PROSITE-ProRule annotationCuratedAdd BLAST30
Domaini854 – 1077Ras-GAPPROSITE-ProRule annotationAdd BLAST224

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili734 – 770Sequence analysisAdd BLAST37
Coiled coili1330 – 1364Sequence analysisAdd BLAST35

Sequence similaritiesi

Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 6 IQ domains.PROSITE-ProRule annotationCurated
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

InParanoidiO14188.
KOiK05767.
OMAiNESSIIA.
OrthoDBiEOG092C0HDM.
PhylomeDBiO14188.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
1.10.506.10. 1 hit.
4.10.270.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR000593. RasGAP_C.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF00612. IQ. 2 hits.
PF00616. RasGAP. 1 hit.
PF03836. RasGAP_C. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00015. IQ. 8 hits.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF48350. SSF48350. 2 hits.
PROSITEiPS50021. CH. 1 hit.
PS50096. IQ. 6 hits.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14188-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVNVGLSRL QSQAGAPVGT KGSNTRLAAK QRETLQAYDY LCRVDEAKKW
60 70 80 90 100
IEECLGTDLG PTSTFEQSLR NGVVLALLVQ KFQPDKLIKI FYSNELQFRH
110 120 130 140 150
SDNINKFLDF IHGIGLPEIF HFELTDIYEG KNLPKVIYCI HALSYFLSMQ
160 170 180 190 200
DLAPPLIKSD ENLSFTDEDV SIIVRRLRQS NVILPNFKAL SADFMLRASP
210 220 230 240 250
VSSRTPSPTR FPKHARFQTL NSSDSASIYS SPYTSPTLEF SKKDASARSD
260 270 280 290 300
ILKMHRRTKS ATPSLEQFNE PYKQTLPSHS IEFEDSFFQP PSQKGHMQRS
310 320 330 340 350
FLTTFSAPTR RREALFSTTS GLSQRSPVDE KIVNAIQACG RGVLVRLRLV
360 370 380 390 400
DMLQSLVEQS SSVVLLQAVI RGYISRNTYR IRKKAYDELV NWVTSIQSIS
410 420 430 440 450
RAYLIRAQYR KVVLQEEATK SIQTLQSIIR GGFYRRKYHS LIERLDLFTP
460 470 480 490 500
SFVLIQSSAL GFLTRHAIVN MLDNLYNYIP LFNRMQSILR ANMFRNEWSN
510 520 530 540 550
FLDSVQSFPV SFHSICKGRL IRDSINRLNG SLLGELDNFI KLQNLSRGFM
560 570 580 590 600
IRRAFKEKLE KLKASTSSFI ALQAIVRAFL LRKNLESIYD SFQKSHLSVI
610 620 630 640 650
KAQSLYRGFI TRTKIDYCND YLLKRLPDIV FMQSAVRAIL LRDDVNYTEV
660 670 680 690 700
QLDSFIPEIV LLQSLIRGYL SRNKFSRKLQ NFHKNMENPI VAKSIFRGRQ
710 720 730 740 750
EGLAYRELAT AKNPPVMTVK NFVHLLDDTN FDFEEEVLLE KMRKEIVQQV
760 770 780 790 800
RDNEEIEVHI NELDVKIALL VKNKISLDDV LKHHNKYKFG KQSTEYLKIN
810 820 830 840 850
TLSMKSLNNS SRKFLELYQC FFYVLQTNEM YLANYFQALK TEGTSSVKIR
860 870 880 890 900
HAVYLVLQIF GHGSNRREEV LLLRFISQVI KLEAALVNSS QDLLSDDCVW
910 920 930 940 950
KLLFTGYRGD VREVKLWKTI LGRIHKVLVA DNHLDFEINP LTLFKSFNPE
960 970 980 990 1000
VASQTDSPKL TLSLAMQHPP TRNLYVSRLR ELRKLCQSFL VALSKNIENI
1010 1020 1030 1040 1050
PYALCYTAAQ LKNSLQRYFP AAHKEEIFGV IGKFVYWAYV APVLVSPDNF
1060 1070 1080 1090 1100
KLVDGSITAL QRKNLYTLSS ILSEIFSIES CDSKQLGFFR PLSEFIEVSK
1110 1120 1130 1140 1150
QDTMLMLERL VDVVDPEVYF EFDAFEDLVN TKRPVIYMKR DDILGIYSSI
1160 1170 1180 1190 1200
AYVIDSIAPP DVNDPLRAVV NSLGPVSEQD NDFVQDETDV KLELNPKFCT
1210 1220 1230 1240 1250
IENPVAQERT LIVQTKRYIL FIIRIQNGLN LLEILVKPVT DSDEAAWQNL
1260 1270 1280 1290 1300
LAEESEKNAR NYDLFDDSIF SMSFAELKYT ALSNIVEMEK LGFANRRNNY
1310 1320 1330 1340 1350
QDMVNSIALD IRNKSRRRMQ RQRELDAGHQ SLLNLREKRA FLDSQLKSYN
1360 1370 1380 1390 1400
EYIEQAMETL QSKKGKKKLI PFSKQYFHMR DLRKSGRVPR FGSFKYPALK
1410 1420 1430 1440 1450
LYDRGVLVSI SHMPQKEKLY ITISADEVGK FILEATSPTV KVSSPRCELH
1460 1470 1480
LDDLLSAQYN KVLTLDVLDG RLKLNTNMFL HLIFSKFYS
Length:1,489
Mass (Da):171,677
Last modified:January 1, 1998 - v1
Checksum:i7D357FF9A7FED5EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11059.1.
AB027779 Genomic DNA. Translation: BAA87083.1.
PIRiT38842.
RefSeqiNP_593860.1. NM_001019289.2.

Genome annotation databases

EnsemblFungiiSPAC4F8.13c.1; SPAC4F8.13c.1:pep; SPAC4F8.13c.
GeneIDi2543129.
KEGGispo:SPAC4F8.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11059.1.
AB027779 Genomic DNA. Translation: BAA87083.1.
PIRiT38842.
RefSeqiNP_593860.1. NM_001019289.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P2XX-ray2.21A32-190[»]
1P5SX-ray2.22A1-190[»]
ProteinModelPortaliO14188.
SMRiO14188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279561. 44 interactors.
IntActiO14188. 2 interactors.
MINTiMINT-4671212.

Proteomic databases

MaxQBiO14188.
PRIDEiO14188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4F8.13c.1; SPAC4F8.13c.1:pep; SPAC4F8.13c.
GeneIDi2543129.
KEGGispo:SPAC4F8.13c.

Organism-specific databases

EuPathDBiFungiDB:SPAC4F8.13c.
PomBaseiSPAC4F8.13c. rng2.

Phylogenomic databases

InParanoidiO14188.
KOiK05767.
OMAiNESSIIA.
OrthoDBiEOG092C0HDM.
PhylomeDBiO14188.

Enzyme and pathway databases

ReactomeiR-SPO-5626467. RHO GTPases activate IQGAPs.
R-SPO-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiO14188.
PROiO14188.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
1.10.506.10. 1 hit.
4.10.270.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR000593. RasGAP_C.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF00612. IQ. 2 hits.
PF00616. RasGAP. 1 hit.
PF03836. RasGAP_C. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00015. IQ. 8 hits.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF48350. SSF48350. 2 hits.
PROSITEiPS50021. CH. 1 hit.
PS50096. IQ. 6 hits.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNG2_SCHPO
AccessioniPrimary (citable) accession number: O14188
Secondary accession number(s): Q9USG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.