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Protein

WD repeat-containing protein pop2

Gene

pop2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maintenance of ploidy through proteasome dependent degradation of CDK inhibitor rum1 and S-phase initiator cdc18. Functions as a recognition factor for rum1 and cdc18, which are subsequently ubiquitinated and targeted to the 26S proteasome for degradation. Together with pop1, required for cig2 instability during G2 and M phase and cig2 degradation in exponentially growing cells.4 Publications

GO - Molecular functioni

  • protein binding, bridging Source: PomBase

GO - Biological processi

  • negative regulation of mitotic DNA replication initiation Source: PomBase
  • protein modification by small protein conjugation or removal Source: PomBase
  • ubiquitin-dependent protein catabolic process Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein pop2
Alternative name(s):
Proteolysis factor sud1
Gene namesi
Name:pop2
Synonyms:sud1
ORF Names:SPAC4D7.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4D7.03.
PomBaseiSPAC4D7.03. pop2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear envelope Source: PomBase
  • SCF ubiquitin ligase complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 703703WD repeat-containing protein pop2PRO_0000051140Add
BLAST

Proteomic databases

MaxQBiO14170.

Interactioni

Subunit structurei

Homodimer and heterodimer with pop1. Binds to cul1, pip1 and phosphorylated cdc18.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
cdc18P414113EBI-1185414,EBI-1207853
cul1O137903EBI-1185414,EBI-1154807
pop1P8706012EBI-1185414,EBI-1185389
skp1Q9Y7093EBI-1185414,EBI-1172248

GO - Molecular functioni

  • protein binding, bridging Source: PomBase

Protein-protein interaction databases

BioGridi279932. 70 interactions.
IntActiO14170. 6 interactions.

Structurei

3D structure databases

ProteinModelPortaliO14170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini236 – 28348F-boxPROSITE-ProRule annotationAdd
BLAST
Repeati389 – 41729WD 1Add
BLAST
Repeati429 – 47345WD 2Add
BLAST
Repeati505 – 53329WD 3Add
BLAST
Repeati545 – 57531WD 4Add
BLAST
Repeati587 – 61529WD 5Add
BLAST
Repeati625 – 65430WD 6Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 170170Interaction with pop1Add
BLAST

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

InParanoidiO14170.
KOiK03361.
OMAiCLQHDDE.
OrthoDBiEOG7X0VRS.
PhylomeDBiO14170.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00646. F-box. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00256. FBOX. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSRCPTDN SSSRINSSVP LINSSSPATP PESFDPQVFP SSLIHGDNLL
60 70 80 90 100
PQDDQIASDP RSESNSCNGN TSSSLPCTDS YQYPLKHSCT PSFLRKFNES
110 120 130 140 150
IENVSYKCLD HSPPDSVPGD FSISLVPQRN FLYSHSSLPP KIISIDRNNR
160 170 180 190 200
IKLDNSISSN SDNFPPSPKV DTSNTVSPGS KPISEDLEDL NLQSIVQTFE
210 220 230 240 250
DLPEGIQSYA FFQLLRSCNR QSMRLLLNEC EPLLKKDILS NLPFSIVQSI
260 270 280 290 300
LLNLDIHSFL SCRLVSPTWN RILDVHTSYW KHMFSLFGFQ INENDWKYAN
310 320 330 340 350
PNLNRPPFLH NDQISDDYFP EIFKRHFLNR KRWLFPSIPP SHLSFPIHVP
360 370 380 390 400
NFMITSLLLH KDRIITTSGS GTIQIHNAIT GVLEARLEGH KEGVWAVKIH
410 420 430 440 450
ENTLVSGSID KTVRVWNIEK AKCTHIFRGH ISIIRCLEIL VPSRLIRHGV
460 470 480 490 500
EIVEPDQPYI VSGSRDHTLR VWKLPKNTDP PYLPDNTNSI DRWEKNPYFV
510 520 530 540 550
HTLIGHTDSV RTISGYGDIL VSGSYDSSIR IWRVSTGECL YHLRGHSLRI
560 570 580 590 600
YSVLYEPERN ICISGSMDKS IRVWDLSTGT CKYVLEGHDA FVTLLNVFQN
610 620 630 640 650
RLISGSADST IRIWDLNTGK PLMVLPSNSG YISSFVSDEH KIISGNDGSV
660 670 680 690 700
KLWDVRTGKL LRFLLTDLTK IWHVDFDAMR CVAAVQRDDQ AYLEVINFSG

SRP
Length:703
Mass (Da):79,605
Last modified:January 1, 1998 - v1
Checksum:i0336A0568C152396
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038867 mRNA. Translation: AAB95480.1.
AF064515 Genomic DNA. Translation: AAC39496.1.
CU329670 Genomic DNA. Translation: CAB11275.1.
PIRiT43557.
RefSeqiNP_594956.1. NM_001020387.2.

Genome annotation databases

EnsemblFungiiSPAC4D7.03.1; SPAC4D7.03.1:pep; SPAC4D7.03.
GeneIDi2543514.
KEGGispo:SPAC4D7.03.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038867 mRNA. Translation: AAB95480.1.
AF064515 Genomic DNA. Translation: AAC39496.1.
CU329670 Genomic DNA. Translation: CAB11275.1.
PIRiT43557.
RefSeqiNP_594956.1. NM_001020387.2.

3D structure databases

ProteinModelPortaliO14170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279932. 70 interactions.
IntActiO14170. 6 interactions.

Proteomic databases

MaxQBiO14170.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4D7.03.1; SPAC4D7.03.1:pep; SPAC4D7.03.
GeneIDi2543514.
KEGGispo:SPAC4D7.03.

Organism-specific databases

EuPathDBiFungiDB:SPAC4D7.03.
PomBaseiSPAC4D7.03. pop2.

Phylogenomic databases

InParanoidiO14170.
KOiK03361.
OMAiCLQHDDE.
OrthoDBiEOG7X0VRS.
PhylomeDBiO14170.

Enzyme and pathway databases

ReactomeiR-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiO14170.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR001810. F-box_dom.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00646. F-box. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00256. FBOX. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "F-box/WD-repeat proteins pop1p and Sud1p/Pop2p form complexes that bind and direct the proteolysis of cdc18p."
    Wolf D.A., McKeon F., Jackson P.K.
    Curr. Biol. 9:373-376(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH POP1 AND CDC18.
    Strain: 972 / ATCC 24843.
  2. "sud1+ targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast."
    Jallepalli P.V., Tien D., Kelly T.J.
    Proc. Natl. Acad. Sci. U.S.A. 95:8159-8164(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH CDC18.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Two F-box/WD-repeat proteins Pop1 and Pop2 form hetero- and homo-complexes together with cullin-1 in fission yeast SCF (Skip-cullin-1-F-box) ubiquitin ligase."
    Kominami K., Ochotorena I., Toda T.
    Genes Cells 3:721-735(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
    Strain: 972 / ATCC 24843.
  5. "Combinatorial diversity of fission yeast SCF ubiquitin ligases by homo- and heterooligomeric assemblies of the F-box proteins Pop1p and Pop2p."
    Seibert V., Prohl C., Schoultz I., Rhee E., Lopez R., Abderazzaq K., Zhou C., Wolf D.A.
    BMC Biochem. 3:22-22(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  6. "Requirement of the SCFPop1/Pop2 ubiquitin ligase for degradation of the fission yeast S phase cyclin Cig2."
    Yamano H., Kominami K., Harrison C., Kitamura K., Katayama S., Dhut S., Hunt T., Toda T.
    J. Biol. Chem. 279:18974-18980(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPOP2_SCHPO
AccessioniPrimary (citable) accession number: O14170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.