O14160 (SYVM_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Valine--tRNA ligase, mitochondrial
EC=6.1.1.9

Alternative name(s):
Valyl-tRNA synthetase
Short name=ValRS

Gene names

Name:	vas1
ORF Names:	SPAC4A8.08c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	950 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).
Subcellular location	Mitochondrion Ref.2.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial valyl-tRNA aminoacylation Inferred from experiment PubMed 20106903. Source: PomBase regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	mitochondrion Inferred from direct assay Ref.2. Source: PomBase
Molecular_function	ATP binding Inferred from sequence model. Source: PomBase aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro valine-tRNA ligase activity Inferred from direct assay PubMed 20106903. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 90	90	Mitochondrion
	Chain	91 – 950	860	Valine--tRNA ligase, mitochondrial		PRO_0000352849
Regions
	Motif	67 – 77	11	"HIGH" region
	Motif	556 – 560	5	"KMSKS" region
Sites
	Binding site	559	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O14160 [UniParc]. Last modified May 1, 1999. Version 2. Checksum: 923B080350E3F40B	FASTA	950	109,627
	10 20 30 40 50 60 MFHFQRSFSS RKAHGLLSFK NRNYIHIEAP FDIAAIQDGW NIIRKHIHYP KPKSIEAPMF 70 80 90 100 110 120 PILLPPPNIT GKLHIGHALT ITIQDALARF YAMHGYKVSF RPGTDHAGIA TQSVVEKYLQ 130 140 150 160 170 180 KKGVYRNQLS KDELLSSIHS WQVKYQKSII NQLKSFEAIF DWDNIFYTMD QNRSEAVNEA 190 200 210 220 230 240 FISLFNAGLI YRANRFVNWC PKLESAVSDI EVESQQINKP VTKYVDNTPV EFGWLYEISY 250 260 270 280 290 300 QLEGSDNEQL NVSTTRPETI FGDRAIAVSP HDERYKKYVG RFVKHPLIDD LLIPVICDNA 310 320 330 340 350 360 VDRHFGTGVL KITPMHSIVD YEIAKRHNID CVSIMDKSGN LINCSKEVNG MNRLKARSKI 370 380 390 400 410 420 VRLLQQRNRL VEQVPHSLIL SVCSRTGDVI EPVMVPQWYL SVDSLKKEVL KSSNKLKLVP 430 440 450 460 470 480 SLARKEWDSW FKKMGDWCIS RQIWWGHQIP VWKILEEDKW IAAPNYEKAL QLSVGKSVSQ 490 500 510 520 530 540 DSDVLDTWFS SALLPLSAFG WPKSKDIQPL PFIESGQDIL FFWIARMALL CKYFSNELPF 550 560 570 580 590 600 KEIILHPLVR DSEGRKMSKS LGNVIDPMDI INGVTLENMK KALLEGNLPI SEVHKSSKQM 610 620 630 640 650 660 EKAFPNGIPA QGIDIFRYGL FLCLHHEQRI LLDMNSFSDA HRFVSKLWNL ARYFNQYKDK 670 680 690 700 710 720 ENPLKLTDSQ RQRISLLKMA TYSKLHHAVE GVKESFEQRK FFNAADIMKN FLLNDLSSVY 730 740 750 760 770 780 VELTRFDVKD SKSSAYEVYR VFSDILHIFL KLIHPIVPCI SGVMLHSKII PERKNSEFLS 790 800 810 820 830 840 FPTSQQECLL VHDNQAEVVV QNAYDVLIQL RKLESPLNKS PSREHTVYIS TSLEPLKYFY 850 860 870 880 890 900 DAIEQSTNLK LKSISQEDTI DLMRNQTFIL SRISSDTILL VPKKLYPSKR KKLRKNLDDL 910 920 930 940 950 QKKLDKIQHT TSSSGYEKAP SYIKLKNCEL QKDILVKIQD IKQALLNTEI

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB38577.1.
PIR	T38777.
RefSeq	NP_593819.1. NM_001019249.2.
3D structure databases
HSSP	HSSP built from PDB template 1WKA based on UniProtKB P96142.
ProteinModelPortal	O14160.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC4A8.08c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC4A8.08c.1; SPAC4A8.08c.1:pep; SPAC4A8.08c.
GeneID	2543603.
KEGG	spo:SPAC4A8.08c.
Organism-specific databases
PomBase	SPAC4A8.08c.
Phylogenomic databases
eggNOG	COG0525.
HOGENOM	HOG000020094.
KO	K01873.
OrthoDB	EOG4GQTFZ.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. IPR002303. Valyl-tRNA_ligase. [Graphical view]
PANTHER	PTHR11946:SF5. PTHR11946:SF5. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00986. TRNASYNTHVAL.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00422. valS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804610.

Entry information

Entry name

SYVM_SCHPO

Accession

Primary (citable) accession number: O14160

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program