Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin type-2 heavy chain 2

Gene

myo3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stabilizes the F-actin cables forming the F-actin ring that surrounds the nucleus during interphase. May work in conjunction with myo2.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi182 – 1898ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • mitotic actomyosin contractile ring assembly Source: PomBase
  • mitotic actomyosin contractile ring contraction Source: PomBase
  • mitotic cytokinesis Source: PomBase
  • nuclear migration during mitotic telophase Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin type-2 heavy chain 2
Alternative name(s):
Myosin type II heavy chain 2
Gene namesi
Name:myo3
Synonyms:myp2
ORF Names:SPAC4A8.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4A8.05c.
PomBaseiSPAC4A8.05c.

Subcellular locationi

GO - Cellular componenti

  • actomyosin contractile ring Source: PomBase
  • cell division site Source: PomBase
  • cytoplasm Source: PomBase
  • mitochondrion Source: PomBase
  • myosin II complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21042104Myosin type-2 heavy chain 2PRO_0000123481Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1421 – 14211Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14157.
PRIDEiO14157.

PTM databases

iPTMnetiO14157.

Interactioni

Subunit structurei

Binds to cdc4 and rlc1.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi279991. 56 interactions.
MINTiMINT-4670985.

Structurei

3D structure databases

ProteinModelPortaliO14157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini89 – 767679Myosin motorAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni646 – 66015Actin-bindingSequence analysisAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili829 – 21041276Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiO14157.
KOiK10352.
OMAiKTHNIND.
OrthoDBiEOG77T1CS.
PhylomeDBiO14157.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR008989. Myosin_S1_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF50084. SSF50084. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYLSKNGSN DNNNIIKKLV DAEKHCNAVK DASFDERTWI WIPDSKESFV
60 70 80 90 100
KAWIVEDLGE KYRVKLERDG SERIVDGFDA EKVNPPKFDM VDDMAALTCL
110 120 130 140 150
NEPSVVNNLT QRYEKDLIYT YSGLFLVAVN PYCHLPIYGD DVVRKYQSKQ
160 170 180 190 200
FKETKPHIFG TADAAYRSLL ERRINQSILV TGESGAGKTE TTKKVIQYLT
210 220 230 240 250
SVTDASTSDS QQLEKKILET NPVLEAFGNA QTVRNNNSSR FGKFIRIEFS
260 270 280 290 300
NNGSIVGANL DWYLLEKSRV IHPSSNERNY HVFYQLLRGA DGSLLESLFL
310 320 330 340 350
DRYVDHYSYL KNGLKHINGV DDGKEFQKLC FGLRTLGFDN NEIHSLFLII
360 370 380 390 400
ASILHIGNIE VASDRSGQAR FPSLTQIDQL CHLLEIPVDG FVNAALHPKS
410 420 430 440 450
KAGREWIVTA RTREQVVHTL QSLAKGLYER NFAHLVKRLN QTMYYSQSEH
460 470 480 490 500
DGFIGVLDIA GFEIFTFNSF EQLCINFTNE KLQQFFNHYM FVLEQEEYTQ
510 520 530 540 550
ERIEWDFIDY GNDLQPTIDA IEKSEPIGIF SCLDEDCVMP MATDATFTEK
560 570 580 590 600
LHLLFKGKSD IYRPKKFSSE GFVLKHYAGD VEYDTKDWLE KNKDPLNACL
610 620 630 640 650
AALMFKSTNS HVSSLFDDYS SNASGRDNIE KKGIFRTVSQ RHRRQLSSLM
660 670 680 690 700
HQLEATQPHF VRCIIPNNLK QPHNLDKSLV LHQLRCNGVL EGIRIAQTGF
710 720 730 740 750
PNKLFYTEFR ARYGILSQSL KRGYVEAKKA TITIINELKL PSTVYRLGET
760 770 780 790 800
KVFFKASVLG SLEDRRNALL RVIFNSFSAR IRGFLTRRRL YRFNHRQDAA
810 820 830 840 850
ILLQHNLRQL KLLKPHPWWN LFLHLKPLLG TTQTDEYLRR KDALINNLQN
860 870 880 890 900
QLESTKEVAN ELTITKERVL QLTNDLQEEQ ALAHEKDILV ERANSRVEVV
910 920 930 940 950
HERLSSLENQ VTIADEKYEF LYAEKQSIEE DLANKQTEIS YLSDLSSTLE
960 970 980 990 1000
KKLSSIKKDE QTISSKYKEL EKDYLNIMAD YQHSSQHLSN LEKAINEKNL
1010 1020 1030 1040 1050
NIRELNEKLM RLDDELLLKQ RSYDTKVQEL REENASLKDQ CRTYESQLAS
1060 1070 1080 1090 1100
LVSKYSETES ELNKKEAELV IFQKEITEYR DQLHKAFQNP EKTHNINDVK
1110 1120 1130 1140 1150
SGPLNSDENI YSTSSTTLSI LKDVQELKSL HTKEANQLSE RIKEISEMLE
1160 1170 1180 1190 1200
QSIATEEKLR RKNSELCDII EALKYQIQDQ ETEIISLNAD NLDLKDTNGV
1210 1220 1230 1240 1250
LEKNASDFID FQGIKSRYEH KISDLLNQLQ KERCKVGLLK QKTENRSVTQ
1260 1270 1280 1290 1300
HTLDGNSPHP SFEEKHSGDP LKRIDGNNDD RKIDNKLLKT ISKSLDALQL
1310 1320 1330 1340 1350
TVEEELSNLY SLSKDLSFTD ISGHIPNSIR KLEKGLSTLS ELKERLNASN
1360 1370 1380 1390 1400
SDRPSPDIFK DTQAIMNSRK LLSNPNSDAQ SGLISSLQKK LYNPESNMEF
1410 1420 1430 1440 1450
TGLKPLSPSK ISNLPSSQPG SPSKRSGKME ALIRNFDQNS SIPDPFIVNQ
1460 1470 1480 1490 1500
RNSVLQTEFE KINLKLKEAT KSGILDNKDL SKFSELIQSL LKENEELKNL
1510 1520 1530 1540 1550
TTSNLGSDDK MLDFAPLLED VPNNTRNQIK GFVEKAISSK RAIAKLYSAS
1560 1570 1580 1590 1600
EEKLFSTEKA LREITKERDR LLHGLQGPSV PTSPLKAPTA SQLIIPNFDG
1610 1620 1630 1640 1650
SITNYSGEEE TEWLQEEVNI MKIKELTSTV NKYREQLAMV QSLNEHAESS
1660 1670 1680 1690 1700
LSKAERSKNY LTGRLQEVEE LARGFQTTNA DLQNELADAV VKQKEYEVLY
1710 1720 1730 1740 1750
VEKSNDYNTL LLQKEKLMKQ IDEFHVIRVQ DLEEREKKDQ LLFQRYQKEL
1760 1770 1780 1790 1800
NGFKVQLEEE REKNLRIRQD NRHMHAEIGD IRTKFDELVL EKTNLLKENS
1810 1820 1830 1840 1850
ILQADLQSLS RVNNSSSTAQ QNAQSQLLSL TAQLQEVREA NQTLRKDQDT
1860 1870 1880 1890 1900
LLRENRNLER KLHEVSEQLN KKFDSSARPF DEIEMEKEVL TLKSNLAQKD
1910 1920 1930 1940 1950
DLLSSLVERI KQIEMFALKT QKDSNNHREE NLQLHRQLGV LQKEKKDLEL
1960 1970 1980 1990 2000
KLFDLDLKTY PISTSKDVRM LQKQISDLEA SFAASDIERI KGIDECRNRD
2010 2020 2030 2040 2050
RTIRQLEAQI SKFDDDKKRI QSSVSRLEER NAQLRNQLED VQASETQWKF
2060 2070 2080 2090 2100
ALRRTEHALQ EERERVKSLE TDFDKYRSLL EGQRVKRSES RLSMRSNRSP

SVLR
Length:2,104
Mass (Da):242,572
Last modified:January 1, 1998 - v1
Checksum:i3A0548594028D258
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1193 – 11931D → G in AAC04615 (PubMed:9398685).Curated
Sequence conflicti1304 – 13041E → G in AAC04615 (PubMed:9398685).Curated
Sequence conflicti1344 – 13441E → K in AAC04615 (PubMed:9398685).Curated
Sequence conflicti1420 – 14201G → D in AAC04615 (PubMed:9398685).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007633 Genomic DNA. Translation: BAA24579.1.
AF029788 Genomic DNA. Translation: AAC04615.1.
CU329670 Genomic DNA. Translation: CAB11475.1.
AB027812 Genomic DNA. Translation: BAA87116.1.
PIRiT38774.
RefSeqiNP_593816.1. NM_001019246.2.

Genome annotation databases

EnsemblFungiiSPAC4A8.05c.1; SPAC4A8.05c.1:pep; SPAC4A8.05c.
GeneIDi2543576.
KEGGispo:SPAC4A8.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007633 Genomic DNA. Translation: BAA24579.1.
AF029788 Genomic DNA. Translation: AAC04615.1.
CU329670 Genomic DNA. Translation: CAB11475.1.
AB027812 Genomic DNA. Translation: BAA87116.1.
PIRiT38774.
RefSeqiNP_593816.1. NM_001019246.2.

3D structure databases

ProteinModelPortaliO14157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279991. 56 interactions.
MINTiMINT-4670985.

PTM databases

iPTMnetiO14157.

Proteomic databases

MaxQBiO14157.
PRIDEiO14157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4A8.05c.1; SPAC4A8.05c.1:pep; SPAC4A8.05c.
GeneIDi2543576.
KEGGispo:SPAC4A8.05c.

Organism-specific databases

EuPathDBiFungiDB:SPAC4A8.05c.
PomBaseiSPAC4A8.05c.

Phylogenomic databases

InParanoidiO14157.
KOiK10352.
OMAiKTHNIND.
OrthoDBiEOG77T1CS.
PhylomeDBiO14157.

Enzyme and pathway databases

ReactomeiR-SPO-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

NextBioi20804583.
PROiO14157.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR008989. Myosin_S1_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF50084. SSF50084. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of Myo3, a second type-II myosin heavy chain in the fission yeast Schizosaccharomyces pombe."
    Motegi F., Nakano K., Kitayama C., Yamamoto M., Mabuchi I.
    FEBS Lett. 420:161-166(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of a second myosin-II in Schizosaccharomyces pombe: Myp2p is conditionally required for cytokinesis."
    Bezanilla M., Forsburg S.L., Pollard T.D.
    Mol. Biol. Cell 8:2693-2705(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1871-2078.
    Strain: ATCC 38364 / 968.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1421, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiMYO3_SCHPO
AccessioniPrimary (citable) accession number: O14157
Secondary accession number(s): O42730, Q9UU49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: January 1, 1998
Last modified: February 17, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.