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Protein

Alpha-amylase mde5

Gene

mde5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051SubstrateBy similarity
Metal bindingi143 – 1431Calcium 1By similarity
Binding sitei144 – 1441SubstrateBy similarity
Metal bindingi182 – 1821Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi195 – 1951Calcium 1By similarity
Binding sitei224 – 2241SubstrateBy similarity
Active sitei226 – 2261NucleophileBy similarity
Metal bindingi226 – 2261Calcium 2By similarity
Metal bindingi230 – 2301Calcium 1; via carbonyl oxygenBy similarity
Active sitei250 – 2501Proton donorBy similarity
Metal bindingi250 – 2501Calcium 2By similarity
Binding sitei254 – 2541Substrate; via amide nitrogenBy similarity
Binding sitei318 – 3181SubstrateBy similarity
Sitei318 – 3181Transition state stabilizerBy similarity
Binding sitei365 – 3651SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase mde5 (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Mei4-dependent protein 5
Meiotic expression up-regulated protein 30
Gene namesi
Name:mde5
Synonyms:meu30
ORF Names:SPAC25H1.09, SPAC4A8.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC25H1.09.
PomBaseiSPAC25H1.09. mde5.

Subcellular locationi

GO - Cellular componenti

  • anchored component of external side of plasma membrane Source: PomBase
  • endoplasmic reticulum Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 513488Alpha-amylase mde5PRO_0000001357Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 60By similarity
Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence analysis
Disulfide bondi171 ↔ 184By similarity
Disulfide bondi260 ↔ 304By similarity
Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi454 ↔ 488By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

BioGridi280575. 10 interactions.
MINTiMINT-4670941.

Structurei

3D structure databases

ProteinModelPortaliO14154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2302Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiO14154.
KOiK01176.
OMAiNQTQVED.
OrthoDBiEOG7RBZJ4.
PhylomeDBiO14154.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHNEVFGWT LKVLSFLLVV IPANALDKHG WRKQSIYSLL TDRFASTNPK
60 70 80 90 100
PCNPEDREYC GGNWRGIIDK LDYIQGMGFT AIWISPIIKN IEGRTKYGEA
110 120 130 140 150
YHGYWPQDLY TLNPHFGTEQ DLIDLADALH DRGMYLMVDT VVNHMGSSDP
160 170 180 190 200
RNIDYGIYRP FNQSSHYHPM CPIEQDKPLS LEQCWIGTED MTLPDIDTEN
210 220 230 240 250
PQIIETLYNF IHDQVKQFKI DGLRVDATKH VRRTFWPGFC ESAGVYCQGE
260 270 280 290 300
EWTGQADLFC EWQEYMDGLH NFPVQGVAAE SVIPLNDRAL RKTAIAMNLV
310 320 330 340 350
AHHCKDSTLL GLFLESQDAP RLAALNNDYT VLKNAMTLNL MSDGIPIVFY
360 370 380 390 400
GQEQMFNGSH DPVNRPALWD QGYNTDGPLY QYTSKVNKIR RDLINSEDGE
410 420 430 440 450
IYIRSITHAI MIGDHVMVMY KGPVITFITN YGAVDKEYLI KMPGSETMID
460 470 480 490 500
LLTCTLIEVE GEVMRTSIKK GEPKILYPYQ LAFRDGFCQE QITLQEIDDV
510
FMGRNEINGP DRK
Length:513
Mass (Da):58,716
Last modified:January 1, 1998 - v1
Checksum:i455DD97FA428C182
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAD62442.1.
AB054314 mRNA. Translation: BAB60880.1.
PIRiT38770.
RefSeqiXP_001713068.1. XM_001713016.2.

Genome annotation databases

EnsemblFungiiSPAC25H1.09.1; SPAC25H1.09.1:pep; SPAC25H1.09.
GeneIDi3361499.
KEGGispo:SPAC25H1.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAD62442.1.
AB054314 mRNA. Translation: BAB60880.1.
PIRiT38770.
RefSeqiXP_001713068.1. XM_001713016.2.

3D structure databases

ProteinModelPortaliO14154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280575. 10 interactions.
MINTiMINT-4670941.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC25H1.09.1; SPAC25H1.09.1:pep; SPAC25H1.09.
GeneIDi3361499.
KEGGispo:SPAC25H1.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC25H1.09.
PomBaseiSPAC25H1.09. mde5.

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiO14154.
KOiK01176.
OMAiNQTQVED.
OrthoDBiEOG7RBZJ4.
PhylomeDBiO14154.

Miscellaneous databases

PROiO14154.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe."
    Watanabe T., Miyashita K., Saito T.T., Yoneki T., Kakihara Y., Nabeshima K., Kishi Y.A., Shimoda C., Nojima H.
    Nucleic Acids Res. 29:2327-2337(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-513.
    Strain: CD16-1.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMDE5_SCHPO
AccessioniPrimary (citable) accession number: O14154
Secondary accession number(s): Q874R5, Q96WR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.