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Protein

Alpha-amylase mde5

Gene

mde5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei105SubstrateBy similarity1
Metal bindingi143Calcium 1By similarity1
Binding sitei144SubstrateBy similarity1
Metal bindingi182Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi195Calcium 1By similarity1
Binding sitei224SubstrateBy similarity1
Active sitei226NucleophileBy similarity1
Metal bindingi226Calcium 2By similarity1
Metal bindingi230Calcium 1; via carbonyl oxygenBy similarity1
Active sitei250Proton donorBy similarity1
Metal bindingi250Calcium 2By similarity1
Binding sitei254Substrate; via amide nitrogenBy similarity1
Binding sitei318SubstrateBy similarity1
Sitei318Transition state stabilizerBy similarity1
Binding sitei365SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase mde5 (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Mei4-dependent protein 5
Meiotic expression up-regulated protein 30
Gene namesi
Name:mde5
Synonyms:meu30
ORF Names:SPAC25H1.09, SPAC4A8.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC25H1.09.
PomBaseiSPAC25H1.09. mde5.

Subcellular locationi

GO - Cellular componenti

  • anchored component of external side of plasma membrane Source: PomBase
  • endoplasmic reticulum Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000135726 – 513Alpha-amylase mde5Add BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 60By similarity
Glycosylationi162N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi171 ↔ 184By similarity
Disulfide bondi260 ↔ 304By similarity
Glycosylationi357N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi454 ↔ 488By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO14154.

Interactioni

Protein-protein interaction databases

BioGridi280575. 10 interactors.
MINTiMINT-4670941.

Structurei

3D structure databases

ProteinModelPortaliO14154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni229 – 230Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiO14154.
KOiK01176.
OMAiNTESPHV.
OrthoDBiEOG092C1HLH.
PhylomeDBiO14154.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHNEVFGWT LKVLSFLLVV IPANALDKHG WRKQSIYSLL TDRFASTNPK
60 70 80 90 100
PCNPEDREYC GGNWRGIIDK LDYIQGMGFT AIWISPIIKN IEGRTKYGEA
110 120 130 140 150
YHGYWPQDLY TLNPHFGTEQ DLIDLADALH DRGMYLMVDT VVNHMGSSDP
160 170 180 190 200
RNIDYGIYRP FNQSSHYHPM CPIEQDKPLS LEQCWIGTED MTLPDIDTEN
210 220 230 240 250
PQIIETLYNF IHDQVKQFKI DGLRVDATKH VRRTFWPGFC ESAGVYCQGE
260 270 280 290 300
EWTGQADLFC EWQEYMDGLH NFPVQGVAAE SVIPLNDRAL RKTAIAMNLV
310 320 330 340 350
AHHCKDSTLL GLFLESQDAP RLAALNNDYT VLKNAMTLNL MSDGIPIVFY
360 370 380 390 400
GQEQMFNGSH DPVNRPALWD QGYNTDGPLY QYTSKVNKIR RDLINSEDGE
410 420 430 440 450
IYIRSITHAI MIGDHVMVMY KGPVITFITN YGAVDKEYLI KMPGSETMID
460 470 480 490 500
LLTCTLIEVE GEVMRTSIKK GEPKILYPYQ LAFRDGFCQE QITLQEIDDV
510
FMGRNEINGP DRK
Length:513
Mass (Da):58,716
Last modified:January 1, 1998 - v1
Checksum:i455DD97FA428C182
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAD62442.1.
AB054314 mRNA. Translation: BAB60880.1.
PIRiT38770.
RefSeqiXP_001713068.1. XM_001713016.2.

Genome annotation databases

EnsemblFungiiSPAC25H1.09.1; SPAC25H1.09.1:pep; SPAC25H1.09.
GeneIDi3361499.
KEGGispo:SPAC25H1.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAD62442.1.
AB054314 mRNA. Translation: BAB60880.1.
PIRiT38770.
RefSeqiXP_001713068.1. XM_001713016.2.

3D structure databases

ProteinModelPortaliO14154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280575. 10 interactors.
MINTiMINT-4670941.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEiO14154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC25H1.09.1; SPAC25H1.09.1:pep; SPAC25H1.09.
GeneIDi3361499.
KEGGispo:SPAC25H1.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC25H1.09.
PomBaseiSPAC25H1.09. mde5.

Phylogenomic databases

HOGENOMiHOG000165530.
InParanoidiO14154.
KOiK01176.
OMAiNTESPHV.
OrthoDBiEOG092C1HLH.
PhylomeDBiO14154.

Miscellaneous databases

PROiO14154.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015340. A_amylase_DUF1966_C.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09260. DUF1966. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDE5_SCHPO
AccessioniPrimary (citable) accession number: O14154
Secondary accession number(s): Q874R5, Q96WR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.