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Protein

Probable aminomethyltransferase, mitochondrial

Gene

gcv1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The glycine cleavage system catalyzes the degradation of glycine.By similarity

Catalytic activityi

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei219SubstrateBy similarity1
Binding sitei248SubstrateBy similarity1
Binding sitei385SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-6783984. Glycine degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable aminomethyltransferase, mitochondrial (EC:2.1.2.10)
Alternative name(s):
Glycine cleavage system T protein
Short name:
GCVT
Gene namesi
Name:gcv1
ORF Names:n313, SPAC31G5.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC31G5.14.
PomBaseiSPAC31G5.14. gcv1.

Subcellular locationi

GO - Cellular componenti

  • glycine cleavage complex Source: PomBase
  • mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000010765? – 387Probable aminomethyltransferase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiO14110.
PRIDEiO14110.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.By similarity

Protein-protein interaction databases

BioGridi279624. 1 interactor.
MINTiMINT-4670555.

Structurei

3D structure databases

ProteinModelPortaliO14110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000239380.
InParanoidiO14110.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG092C3LQP.
PhylomeDBiO14110.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRSAALSIL KRQSSTAASS SLKRTPLYDL HLKEGATIVP FAGFSMPVQY
60 70 80 90 100
KGQTISASHK WTREHSGLFD VSHMVQWFVR GENATAYLES ITPSSLKELK
110 120 130 140 150
PFHSTLSAFT NETGGIIDDT IISKQDENTY YIVTNAACSE KDEANLKKHI
160 170 180 190 200
ENWKGVELER VQGRALIAIQ GPETASVVQK LIPNVDFSVL KFGQSAYVDF
210 220 230 240 250
KGVKCLFSRS GYTGEDGFEV SIPEEVSVDF ASTLLADTRV RPIGLGARDT
260 270 280 290 300
LRLEAGMCLY GSDIDDTTSP VEGSLSWIIG KRRRKEGGFV GSSRILKELK
310 320 330 340 350
DGPSRRRVGF IVEKVPARHG SAVEVDGVEV GQVTSGCPSP TLGKNIAMGY
360 370 380
ISTGLHQVGT PAHIKVRNKL HPAQVVRMPF VETHYYK
Length:387
Mass (Da):42,408
Last modified:January 1, 1998 - v1
Checksum:i68A3CBDCFF84C45A
GO

Sequence cautioni

The sequence BAA12709 differs from that shown. Reason: Frameshift at position 305.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127E → G in BAA12709 (Ref. 1) Curated1
Sequence conflicti255A → S in BAA12709 (Ref. 1) Curated1
Sequence conflicti257 – 261MCLYG → NVAFME in BAA12709 (Ref. 1) Curated5
Sequence conflicti264I → T in BAA12709 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84656 Genomic DNA. Translation: BAA12709.1. Frameshift.
CU329670 Genomic DNA. Translation: CAB11698.1.
PIRiT38631.
RefSeqiNP_594015.1. NM_001019441.2.

Genome annotation databases

EnsemblFungiiSPAC31G5.14.1; SPAC31G5.14.1:pep; SPAC31G5.14.
GeneIDi2543195.
KEGGispo:SPAC31G5.14.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84656 Genomic DNA. Translation: BAA12709.1. Frameshift.
CU329670 Genomic DNA. Translation: CAB11698.1.
PIRiT38631.
RefSeqiNP_594015.1. NM_001019441.2.

3D structure databases

ProteinModelPortaliO14110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279624. 1 interactor.
MINTiMINT-4670555.

Proteomic databases

MaxQBiO14110.
PRIDEiO14110.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC31G5.14.1; SPAC31G5.14.1:pep; SPAC31G5.14.
GeneIDi2543195.
KEGGispo:SPAC31G5.14.

Organism-specific databases

EuPathDBiFungiDB:SPAC31G5.14.
PomBaseiSPAC31G5.14. gcv1.

Phylogenomic databases

HOGENOMiHOG000239380.
InParanoidiO14110.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG092C3LQP.
PhylomeDBiO14110.

Enzyme and pathway databases

ReactomeiR-SPO-6783984. Glycine degradation.

Miscellaneous databases

PROiO14110.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
InterProiIPR006223. GCS_T.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGCST_SCHPO
AccessioniPrimary (citable) accession number: O14110
Secondary accession number(s): Q92364
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.