O14072 (ATC4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cation-transporting ATPase 4
EC=3.6.3.-

Gene names

Name:	cta4
ORF Names:	SPACUNK4.07c, SPAC2E11.07c, SPAPYUK71.01

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1211 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has a role in regulating cation homeostasis. Involved in controlling nuclear calcium ion levels. Required for cytokinesis and stabilizing microtubules. Ref.3
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.2 Ref.3.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily. [View classification]

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	calcium ion transmembrane transport Inferred from sequence or structural similarity. Source: GeneDB_Spombe cellular calcium ion homeostasis Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	integral to endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1211	1211	Cation-transporting ATPase 4		PRO_0000046350
Regions
	Topological domain	1 – 23	23	Cytoplasmic Potential
	Transmembrane	24 – 44	21	Helical; Potential
	Topological domain	45 – 54	10	Extracellular Potential
	Transmembrane	55 – 75	21	Helical; Potential
	Topological domain	76 – 191	116	Cytoplasmic Potential
	Transmembrane	192 – 212	21	Helical; Potential
	Topological domain	213 – 216	4	Extracellular Potential
	Transmembrane	217 – 237	21	Helical; Potential
	Topological domain	238 – 397	160	Cytoplasmic Potential
	Transmembrane	398 – 418	21	Helical; Potential
	Topological domain	419 – 1057	639	Extracellular Potential
	Transmembrane	1058 – 1078	21	Helical; Potential
	Topological domain	1079 – 1100	22	Cytoplasmic Potential
	Transmembrane	1101 – 1121	21	Helical; Potential
	Topological domain	1122 – 1136	15	Extracellular Potential
	Transmembrane	1137 – 1157	21	Helical; Potential
	Topological domain	1158 – 1174	17	Cytoplasmic Potential
	Transmembrane	1175 – 1195	21	Helical; Potential
	Topological domain	1196 – 1211	16	Extracellular Potential
Sites
	Active site	485	1	4-aspartylphosphate intermediate By similarity
	Metal binding	824	1	Magnesium By similarity
	Metal binding	828	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	O14072 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 6FEE4228CA5A57EC	FASTA	1,211	136,261
	10 20 30 40 50 60 MGSKALITSP DISSGQLYIK LPTFFHLYVW PFALFVYPYI GYVYQNKLYS EEVRYLTYIA 70 80 90 100 110 120 VGTIHALFWL AGEWNTKVYC LMTCRKTDKV EQATHILVTP SKIGESSSVE PITKLVLPDS 130 140 150 160 170 180 QTIQYSFSFQ RKRFIYEPEK GCFANITFPM DEPSTIGTLK KSTGLTNIQS EIFLYRYGKN 190 200 210 220 230 240 CFDIPIPTFG TLFKEHAVAP FFVFQIFCCV LWCLDDYWYF SLFSMFMIIA LECSVVWQRQ 250 260 270 280 290 300 RTLTEFRTMS IKPYEIQVYR NKHWFPISTE DLLPNDVVSV LHNKEDSGLP CDLLLLSGSC 310 320 330 340 350 360 VVNEAMLSGE STPLVKESIE LRPEEAVIDV DELDKNAVLF GGTRVLQVTQ SPFCKLKTPD 370 380 390 400 410 420 NGVPAIVLRT GFETSQGSLV RTMVFSSEKV TANNRESLYF ILFLLVFAIA ASGYVWHVGS 430 440 450 460 470 480 KTERSRYKLM LDCVMIITSV VPSELPMELS MAVNASLGAL SKYYIYCTEP FRIPLSGHLD 490 500 510 520 530 540 ICCFDKTGTL TEEHMVVQGI AGVNRKDPYS LEKLSDASND AILAIATAHT LVLLEQEGET 550 560 570 580 590 600 PKVVGDPMEK ATVENLGWSI EKKNFVSAPE GSVFYKGKVQ IIRNFQFSSA LKRQSSVSNV 610 620 630 640 650 660 RVSGGSFKTF VSVKGAPEVI ATMLREVPKD YEKIYKDYGR KGSRVLALGY KYFKNYIPEN 670 680 690 700 710 720 QVSDLSRESI ESDLVFAGFL IFTSPLKEDA RQTVQMLNNS SHRCMMITGD NPLTAVYVAE 730 740 750 760 770 780 QVGIVEKPTL VLDIKHENEK ILEWKSTDDT INLPMNPHKS LEASLYEKYD LCITGRALSQ 790 800 810 820 830 840 IINPDVIMSI FTHAWVYARV SPSQKEFMIS TLKHNGYITL MCGDGTNDVG ALKQAHVGVA 850 860 870 880 890 900 LLNASEEDML EMQERARNQK LMGVYEKQIQ LAKRFNLPTP PVPPALCHAF PPGPNNPHRE 910 920 930 940 950 960 KTQEGLNKVL EDLETKKASD VQLTEAEKAA ERRANLANKM FDTLANASDD EAPKLKLGDA 970 980 990 1000 1010 1020 SVAAPFTSKL AVVSSITNIV RQGRCTLVAL VQMHKILALN CLITAYSLSV LHLDGIKFGD 1030 1040 1050 1060 1070 1080 TQYTISGMLM SVCFYCVSRA RPLETLSKER PQAGIFNTYI IGSVLGQFAI HIVTLIYITR 1090 1100 1110 1120 1130 1140 VVYLYEDPLE KVDLEETFKP SLLNTAIYLL QLIQQVSTFA INYQGRPFRE ALSENKGMYY 1150 1160 1170 1180 1190 1200 GLLGIAFVAI AGVTEFSPEL NAKLQLVKMA YNFQIQLLAT MVVDYAACWI IEELMKKYFR 1210 DNKPKEIVLR N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 617-809, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[3]	"The endoplasmic reticulum cation P-type ATPase Cta4p is required for control of cell shape and microtubule dynamics." Okorokova Facanha A.L., Appelgren H., Tabish M., Okorokov L., Ekwall K. J. Cell Biol. 157:1029-1039(2002) [PubMed: 12058018] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA20137.1. AB027853 Genomic DNA. Translation: BAA87157.1.
PIR	T41702.
RefSeq	NP_593971.1. NM_001019398.1.
3D structure databases
ProteinModelPortal	O14072.
ModBase	Search...
Protein-protein interaction databases
STRING	O14072.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPACUNK4.07c.1; SPACUNK4.07c.1:pep; SPACUNK4.07c.
GeneID	2542381.
GenomeReviews	Gene locus cta4 in contig CU329670_GR.
KEGG	spo:SPACUNK4.07c.
NMPDR	fig|4896.1.peg.3941.
Organism-specific databases
GeneDB_Spombe	SPACUNK4.07c.
Phylogenomic databases
eggNOG	fuNOG05237.
GeneTree	EFGT00050000000742.
HOGENOM	HBG316596.
OMA	VISCPLK.
OrthoDB	EOG43XZBM.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002019-MONOMER.
Gene expression databases
ArrayExpress	O14072.
Family and domain databases
InterPro	IPR023306. ATPase_cation_domN. IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006544. ATPase_P-typ_unknown-pump-sp. IPR023214. HAD-like_dom. IPR000150. Hypothet_cof. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K14950.
Pfam	PF00122. E1-E2_ATPase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01494. ATPase_P-type. 2 hits. TIGR01657. P-ATPase-V. 1 hit.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATC4_SCHPO

Accession

Primary (citable) accession number: O14072
Secondary accession number(s): Q9USD0, Q9UT01

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program