O14045 (TPT1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative tRNA 2'-phosphotransferase
EC=2.7.1.160

Gene names

ORF Names:

SPAC2C4.12c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate By similarity.
Catalytic activity	2'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP ribose 1'',2''-phosphate + nicotinamide + H2O.
Sequence similarities	Belongs to the KptA/TPT1 family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	NAD
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA splicing, via endonucleolytic cleavage and ligation Inferred from sequence or structural similarity. Source: PomBase
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: PomBase
Molecular_function	tRNA 2'-phosphotransferase activity Inferred from sequence or structural similarity. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Putative tRNA 2'-phosphotransferase		PRO_0000316237

Sequences

	Sequence		Length	Mass (Da)
	O14045 [UniParc]. Last modified April 18, 2012. Version 2. Checksum: 32F645818B1B7997	FASTA	365	41,028
	10 20 30 40 50 60 MYKNMNSHEL IEESNNSGTP ATKSSSKPTK KIRPRNDLVH YSKALSKVLR HTAKANGLQI 70 80 90 100 110 120 REDGYIEVDS ILKLPQFRGM GMELLLSIVK GNDKKRFTME EVEGVLYIRA NQGHSIKAVQ 130 140 150 160 170 180 VPMARIDNAS SIPKVVHGTK KELWPVISKQ GLSRMKRNHI HCATGLYGDP GVISGIRKSC 190 200 210 220 230 240 TLYIYIDSAK AMQDGVEFYR SENGVILTEG VNGLLSSKYF SRVETSDGEV LLDAKASPKN 250 260 270 280 290 300 NRSDESDQSD PESIDPFCDN LQALSMHELE LLEEKHSNFG YSEGIIKGKM QVAQSGFDDG 310 320 330 340 350 360 FKHGSRLGFQ MGKTIGTLKA KLYIFEENEQ MEILKQELDR LQESAEFHIF VANHKEEILK CIREK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Comparative functional genomics of the fission yeasts." Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K. , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C. Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVISION OF GENE MODEL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB16372.2.
PIR	T38523.
RefSeq	NP_594515.2. NM_001019944.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC2C4.12c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC2C4.12c.1; SPAC2C4.12c.1:pep; SPAC2C4.12c.
GeneID	2541572.
KEGG	spo:SPAC2C4.12c.
Organism-specific databases
PomBase	SPAC2C4.12c.
Phylogenomic databases
eggNOG	COG1859.
KO	K10669.
OrthoDB	EOG418FXT.
Family and domain databases
InterPro	IPR019191. Essential_protein_Yae1_N. IPR002745. Ptrans_KptA/Tpt1. [Graphical view]
Pfam	PF01885. PTS_2-RNA. 1 hit. PF09811. Yae1_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802666.

Entry information

Entry name

TPT1_SCHPO

Accession

Primary (citable) accession number: O14045

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 18, 2012
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program