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Protein

Glutathione transporter 1

Gene

pgt1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity glutathione transporter which plays a role in scavenging glutathione from the extracellular environment for the maintenance of sulfur homeostasis.1 Publication

Kineticsi

  1. KM=63 µM for glutathione1 Publication

    GO - Molecular functioni

    • glutathione transmembrane transporter activity Source: PomBase

    GO - Biological processi

    • glutathione import across plasma membrane Source: PomBase
    • glutathione import into cell Source: PomBase
    • glutathione transmembrane transport Source: PomBase
    • protein transport Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Biological processi

    Peptide transport, Protein transport, Transport

    Protein family/group databases

    TCDBi2.A.67.1.5. the oligopeptide transporter (opt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione transporter 1
    Gene namesi
    Name:pgt1
    ORF Names:SPAC29B12.10c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome I

    Organism-specific databases

    EuPathDBiFungiDB:SPAC29B12.10c.
    PomBaseiSPAC29B12.10c. pgt1.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
    Transmembranei179 – 199HelicalSequence analysisAdd BLAST21
    Transmembranei259 – 279HelicalSequence analysisAdd BLAST21
    Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
    Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
    Transmembranei405 – 425HelicalSequence analysisAdd BLAST21
    Transmembranei480 – 500HelicalSequence analysisAdd BLAST21
    Transmembranei531 – 551HelicalSequence analysisAdd BLAST21
    Transmembranei560 – 580HelicalSequence analysisAdd BLAST21
    Transmembranei592 – 612HelicalSequence analysisAdd BLAST21
    Transmembranei642 – 662HelicalSequence analysisAdd BLAST21
    Transmembranei711 – 731HelicalSequence analysisAdd BLAST21
    Transmembranei757 – 777HelicalSequence analysisAdd BLAST21
    Transmembranei791 – 811HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    • endoplasmic reticulum Source: PomBase
    • endoplasmic reticulum membrane Source: PomBase
    • integral component of plasma membrane Source: PomBase
    • plasma membrane Source: PomBase
    • plasma membrane of cell tip Source: PomBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003159221 – 851Glutathione transporter 1Add BLAST851

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi32N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi77N-linked (GlcNAc...)Sequence analysis1
    Modified residuei93Phosphoserine1 Publication1
    Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi256N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi452N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi464N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi691N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi843N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiO14031.
    PRIDEiO14031.

    PTM databases

    iPTMnetiO14031.

    Expressioni

    Inductioni

    Expression is increased by cadmium ans repressed by cysteine.2 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi278559. 30 interactors.
    MINTiMINT-4669927.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Coiled coili105 – 134Sequence analysisAdd BLAST30

    Sequence similaritiesi

    Belongs to the oligopeptide OPT transporter family.Sequence analysis

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000166970.
    InParanoidiO14031.
    OMAiHHNINIQ.
    OrthoDBiEOG092C0W76.
    PhylomeDBiO14031.

    Family and domain databases

    InterProiIPR004648. Oligpept_transpt.
    IPR004813. OPT.
    [Graphical view]
    PfamiPF03169. OPT. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00727. ISP4_OPT. 1 hit.
    TIGR00728. OPT_sfam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O14031-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTARNSASIP TSIRKTSENE VSGDETPAGV GNLSTKTASK TSLTFRQSSS
    60 70 80 90 100
    DESTSSYSGN HHNINIQHHP NRPFRTNSSS FSPNDYSISE SPSKSKKDGV
    110 120 130 140 150
    HVSAVQLDNE TDSEVESEVE ELERELEAIE DSVYPEVRAA VNPTDDVNLP
    160 170 180 190 200
    VNTWRTWVLT TIFVIVFAAV NQFFSLRYPA LSISFIVAQL ILFPLGKLLN
    210 220 230 240 250
    LLPNWKIGYG RFSFYLNSSP FNVKEHAAIT IAVSLTSSTA YATNILSAQT
    260 270 280 290 300
    SFYKQNLSWG YKILIVLTSQ MLGYGFAGLT RRWIVYPAAM IWPQTLVSTV
    310 320 330 340 350
    LFRTLHGNSG NDIGVLKNNR ISANGWTISR YRFFAYVMIG SFVFYWFPGF
    360 370 380 390 400
    IFKGLSYFTV LCWIWPKNRV VNQLFGYNSG LGILPLTFDW QQVVYNSNPL
    410 420 430 440 450
    ASPWWVICNT FGSVVLIFWI VVPILYYKGV WFSNYLPMLS SSTFDHTGVS
    460 470 480 490 500
    YNSSRVLNSD YSFNHTKYES YSPLYMPMSY SMSTALNFAA VTAIFTHCAL
    510 520 530 540 550
    YNGKDIWQRL WKESGKDECI HRKLMRNYKE APQWWYATLF IVVFGLTIFT
    560 570 580 590 600
    VRYYDTQCPV WALIVALLIF IVNFIPQGVL EGITNQHVGL NIITELIGGY
    610 620 630 640 650
    ILPGKPLANL MIKLYGFIPM RQGLEFSRDL KLAQYMKIPP RILFFVQLFA
    660 670 680 690 700
    TILGGITQVA VQEWMNYHIP GICTTSQSNG FTCPNGRSIY NASLIWGAIG
    710 720 730 740 750
    PAKMFSKGKP YYPLIFFFLI GAVAPFITWG LRKRFPKSWI GKLNAPVLFT
    760 770 780 790 800
    GPGNIPPATG INYSSWAIVG FIFNYVIRKR AIHWWRKYNY VLAAAMDSGV
    810 820 830 840 850
    AVAGVVIFLC VSYPGGKITW WGNTVYTKTY DWKSVPYRSL GPNETFGYTN

    W
    Length:851
    Mass (Da):96,302
    Last modified:January 1, 1998 - v1
    Checksum:iEF3F886170FD8401
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAB16254.1.
    PIRiT38497.
    RefSeqiNP_594987.1. NM_001020418.2.

    Genome annotation databases

    EnsemblFungiiSPAC29B12.10c.1; SPAC29B12.10c.1:pep; SPAC29B12.10c.
    GeneIDi2542082.
    KEGGispo:SPAC29B12.10c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAB16254.1.
    PIRiT38497.
    RefSeqiNP_594987.1. NM_001020418.2.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi278559. 30 interactors.
    MINTiMINT-4669927.

    Protein family/group databases

    TCDBi2.A.67.1.5. the oligopeptide transporter (opt) family.

    PTM databases

    iPTMnetiO14031.

    Proteomic databases

    MaxQBiO14031.
    PRIDEiO14031.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiSPAC29B12.10c.1; SPAC29B12.10c.1:pep; SPAC29B12.10c.
    GeneIDi2542082.
    KEGGispo:SPAC29B12.10c.

    Organism-specific databases

    EuPathDBiFungiDB:SPAC29B12.10c.
    PomBaseiSPAC29B12.10c. pgt1.

    Phylogenomic databases

    HOGENOMiHOG000166970.
    InParanoidiO14031.
    OMAiHHNINIQ.
    OrthoDBiEOG092C0W76.
    PhylomeDBiO14031.

    Miscellaneous databases

    PROiO14031.

    Family and domain databases

    InterProiIPR004648. Oligpept_transpt.
    IPR004813. OPT.
    [Graphical view]
    PfamiPF03169. OPT. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00727. ISP4_OPT. 1 hit.
    TIGR00728. OPT_sfam. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPGT1_SCHPO
    AccessioniPrimary (citable) accession number: O14031
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 1, 1998
    Last modified: October 5, 2016
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.