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Protein

Histone-lysine N-methyltransferase, H3 lysine-36 specific

Gene

set2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone methyltransferase that methylates histone H3 to form H3K36me. Involved in transcription elongation as well as in transcription repression.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

GO - Molecular functioni

  • DNA binding Source: InterPro
  • histone methyltransferase activity (H3-K36 specific) Source: PomBase

GO - Biological processi

  • histone H3-K36 methylation Source: PomBase
  • regulation of transcription from RNA polymerase II promoter Source: PomBase
  • transcription elongation from RNA polymerase II promoter Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Repressor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-36 specific (EC:2.1.1.43)
Alternative name(s):
Lysine N-methyltransferase 3
SET domain-containing protein 2
Gene namesi
Name:set2
Synonyms:kmt3
ORF Names:SPAC29B12.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC29B12.02c.
PomBaseiSPAC29B12.02c.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase II, holoenzyme Source: PomBase
  • nuclear chromatin Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 798798Histone-lysine N-methyltransferase, H3 lysine-36 specificPRO_0000269792Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine1 Publication
Modified residuei545 – 5451Phosphothreonine1 Publication
Modified residuei594 – 5941Phosphoserine1 Publication
Modified residuei596 – 5961Phosphoserine1 Publication
Modified residuei783 – 7831Phosphothreonine1 Publication
Modified residuei785 – 7851Phosphothreonine1 Publication
Modified residuei787 – 7871Phosphoserine1 Publication
Modified residuei789 – 7891Phosphoserine1 Publication
Modified residuei793 – 7931Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14026.

Interactioni

Protein-protein interaction databases

BioGridi278547. 22 interactions.
MINTiMINT-4669878.
STRINGi4896.SPAC29B12.02c-1.

Structurei

3D structure databases

ProteinModelPortaliO14026.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 17855AWSPROSITE-ProRule annotationAdd
BLAST
Domaini180 – 297118SETPROSITE-ProRule annotationAdd
BLAST
Domaini304 – 32017Post-SETPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili627 – 67448Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 7857Ser-richAdd
BLAST

Domaini

The AWS and SET domains are necessary for transcription repression.By similarity

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.PROSITE-ProRule annotation
Contains 1 AWS domain.PROSITE-ProRule annotation
Contains 1 post-SET domain.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG2940.
InParanoidiO14026.
KOiK11423.
OrthoDBiEOG7P8PH3.
PhylomeDBiO14026.

Family and domain databases

InterProiIPR006560. AWS_dom.
IPR025788. Hist-Lys_N-MeTrfase_SET2_fun.
IPR003616. Post-SET_dom.
IPR001214. SET_dom.
IPR013257. SRI.
IPR017923. TFIIS_N.
[Graphical view]
PfamiPF00856. SET. 1 hit.
PF08236. SRI. 1 hit.
[Graphical view]
SMARTiSM00570. AWS. 1 hit.
SM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS51215. AWS. 1 hit.
PS50868. POST_SET. 1 hit.
PS51568. SAM_MT43_SET2_1. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTASSLSVL TPLNEENVDR KSSWSKDTIA VQAVGSSPSS SSSHDFESKE
60 70 80 90 100
DAEGMNKDES APSPSTSSPS SASSRSQSKY VRKEALPPQL FHHLDSAKDK
110 120 130 140 150
ALTTFEEIQE CQYASANIGK PPENEAMICD CRPHWVDGVN VACGHGSNCI
160 170 180 190 200
NRMTSIECTD EDNVCGPSCQ NQRFQRHEFA KVDVFLTEKK GFGLRADANL
210 220 230 240 250
PKDTFVYEYI GEVIPEQKFR KRMRQYDSEG IKHFYFMMLQ KGEYIDATKR
260 270 280 290 300
GSLARFCNHS CRPNCYVDKW MVGDKLRMGI FCKRDIIRGE ELTFDYNVDR
310 320 330 340 350
YGAQAQPCYC GEPCCVGYIG GKTQTEAQSK LPENVREALG IEDEEDSWEN
360 370 380 390 400
ITARRQRRKK GIDETSKIIE EVQPTPLTSE SATKVIGVLL QTKDDLLTRK
410 420 430 440 450
LMERIFLTSD PSVCRSIIAL RGYNIFGLML KKFSIDIEFI LRSIKTMLSW
460 470 480 490 500
PRLTRNKIQD SNIEPVVQEF CDHENEEVKD HAKTLLKEWE SLEIAYRIPR
510 520 530 540 550
RKPGQVAPQS TNAEPSNNQS NPPLRDQEPQ RGDKGDIKSA INNSTEDLSK
560 570 580 590 600
KHPALHSSRP SDSRSRSKFG NDYQSHSKHN LFRKNSFPKR RRLSNSDTPS
610 620 630 640 650
ETTTPNNEQE QVSNQANKVD LNKIISAAME SVNQKNVLKA QKEEEERIAQ
660 670 680 690 700
QKREEKRRLA YEESLKRHAK KLHEKKTKSS QDATIDHHLT SHSPESIAFK
710 720 730 740 750
AVLAKFFANK TARYQEKLGK AEFKLRVKKM TEIILKKHIQ LVLSKKEKAL
760 770 780 790
PDELSDSQQR KLRVWAFRYL DTVVSRSGTA TTTPTDSPSI GESPKKAA
Length:798
Mass (Da):90,679
Last modified:January 1, 1998 - v1
Checksum:i4ACEE9D1705A639E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16247.1.
PIRiT38490.
RefSeqiNP_594980.1. NM_001020411.2.

Genome annotation databases

EnsemblFungiiSPAC29B12.02c.1; SPAC29B12.02c.1:pep; SPAC29B12.02c.
GeneIDi2542070.
KEGGispo:SPAC29B12.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16247.1.
PIRiT38490.
RefSeqiNP_594980.1. NM_001020411.2.

3D structure databases

ProteinModelPortaliO14026.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278547. 22 interactions.
MINTiMINT-4669878.
STRINGi4896.SPAC29B12.02c-1.

Proteomic databases

MaxQBiO14026.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC29B12.02c.1; SPAC29B12.02c.1:pep; SPAC29B12.02c.
GeneIDi2542070.
KEGGispo:SPAC29B12.02c.

Organism-specific databases

EuPathDBiFungiDB:SPAC29B12.02c.
PomBaseiSPAC29B12.02c.

Phylogenomic databases

eggNOGiCOG2940.
InParanoidiO14026.
KOiK11423.
OrthoDBiEOG7P8PH3.
PhylomeDBiO14026.

Miscellaneous databases

NextBioi20803143.
PROiO14026.

Family and domain databases

InterProiIPR006560. AWS_dom.
IPR025788. Hist-Lys_N-MeTrfase_SET2_fun.
IPR003616. Post-SET_dom.
IPR001214. SET_dom.
IPR013257. SRI.
IPR017923. TFIIS_N.
[Graphical view]
PfamiPF00856. SET. 1 hit.
PF08236. SRI. 1 hit.
[Graphical view]
SMARTiSM00570. AWS. 1 hit.
SM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS51215. AWS. 1 hit.
PS50868. POST_SET. 1 hit.
PS51568. SAM_MT43_SET2_1. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Histone H3 K36 methylation is associated with transcription elongation in Schizosaccharomyces pombe."
    Morris S.A., Shibata Y., Noma K., Tsukamoto Y., Warren E., Temple B., Grewal S.I.S., Strahl B.D.
    Eukaryot. Cell 4:1446-1454(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67; THR-545; SER-594; SER-596; THR-783; THR-785; SER-787; SER-789 AND SER-793, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSET2_SCHPO
AccessioniPrimary (citable) accession number: O14026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.