Reviewed, UniProtKB/Swiss-Prot O14021 (PRW1_SCHPO)

Names and origin

Protein names

Recommended name:
    RbAp48-related WD40 repeat-containing protein prw1

Gene names

Name:	prw1
ORF Names:	SPAC29A4.18

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a role in chromatin assembly and chromosome segregation. Involved in the deacetylation of histones. Ref.2
Subunit structure	Heterotetramer of alp13, clr6, prw1 and pst2. Ref.2
Subcellular location	Nucleus Ref.2.
Sequence similarities	Belongs to the WD repeat HIR1 family. Contains 6 WD repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat WD repeat
Molecular function	Chromatin regulator
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	chromatin remodeling Inferred by curator. Source: GeneDB_SPombe chromosome condensation Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe histone deacetylation Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Rpd3L complex Inferred from direct assay. Source: GeneDB_SPombe Rpd3L-Expanded complex Inferred from direct assay. Source: GeneDB_SPombe Rpd3S complex Ref.2 Inferred from direct assay. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protein binding Ref.2 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pst2	O13919	1	EBI-904698,EBI-904686

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	RbAp48-related WD40 repeat-containing protein prw1		PRO_0000051170
Regions
	Repeat	127 – 159	33	WD 1
	Repeat	182 – 213	32	WD 2
	Repeat	232 – 263	32	WD 3
	Repeat	279 – 310	32	WD 4
	Repeat	323 – 354	32	WD 5
	Repeat	380 – 411	32	WD 6

Sequences

	Sequence		Length	Mass (Da)
	O14021-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 4AFF1BDA12C4DBC2	FASTA	431	48,476
	10 20 30 40 50 60 MAVSAVPHPS KQAQASEEGI NQEKCINEEY KIWKKNSPFL YDLIITRALE WPCMSLQWYP 70 80 90 100 110 120 EQQIFAEHGY TEQKMFLGVR ADVGKYLLAV ASIQLPYLNQ TVPPTTMEGA SAGDESSLRV 130 140 150 160 170 180 NISNLYSHPE SVCSAKLMPQ DDSCVATVGN YHNDVLVFDK ESFESYSSAS ESPLKPKYRL 190 200 210 220 230 240 TKHTQPCTSV CWNFLSKGTL VSGSQDATLS CWDLNAYNES DSASVLKVHI SSHEKQVSDV 250 260 270 280 290 300 RFHYKHQDLL ASVSYDQYLH VHDIRRPDAS TKPARSVHAH SGPIHSVAFN PHNDFILATC 310 320 330 340 350 360 STDKTIALWD LRNLNQRLHT LEGHEDIVTK ISFSPHEEPI LASTSADRRT LVWDLSRIGE 370 380 390 400 410 420 DQPAEEAQDG PPELLFMHGG HTSCTIDMDW CPNYNWTMAT AAEDNILQIW TPSRSIWGNE 430 QLEEDATAYL S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genomic integrity." Nakayama J., Xiao G., Noma K., Malikzay A., Bjerling P., Ekwall K., Kobayashi R., Grewal S.I.S. EMBO J. 22:2776-2787(2003) [PubMed: 12773392] [Abstract] Cited for: PROTEIN SEQUENCE OF 12-24; 36-47 AND 318-349, IDENTIFICATION, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB10144.1.
PIR	T38471.
RefSeq	NP_594864.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29342N.
IntAct	O14021. 3 interactions.
STRING	O14021.
Genome annotation databases
GeneID	2541804.
GenomeReviews	Gene locus prw1 in contig CU329670_GR.
KEGG	spo:SPAC29A4.18.
NMPDR	fig|4896.1.peg.4834.
Organism-specific databases
GeneDB_Spombe	SPAC29A4.18.
Phylogenomic databases
HOGENOM	HBG435352.
OrthoDB	EOG9QVDVC.
Gene expression databases
ArrayExpress	O14021.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep_reg. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 5 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 6 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRW1_SCHPO

Accession

Primary (citable) accession number: O14021

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	December 15, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)