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Protein

Serine--tRNA ligase, cytoplasmic

Gene

SPAC29A4.15

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (By similarity).By similarity

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).
Proteins known to be involved in this subpathway in this organism are:
  1. Serine--tRNA ligase, mitochondrial (dia4), Serine--tRNA ligase, cytoplasmic (SPAC29A4.15)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei287ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei294SerineBy similarity1
Binding sitei396SerineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi271 – 273ATPBy similarity3
Nucleotide bindingi358 – 361ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00906; UER00895.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11)
Alternative name(s):
Seryl-tRNA synthetase
Short name:
SerRS
Seryl-tRNA(Ser/Sec) synthetase
Gene namesi
ORF Names:SPAC29A4.15
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC29A4.15.
PomBaseiSPAC29A4.15.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001221981 – 450Serine--tRNA ligase, cytoplasmicAdd BLAST450

Proteomic databases

MaxQBiO14018.
PRIDEiO14018.

Interactioni

Subunit structurei

Homodimer. The tRNA molecule binds across the dimer (By similarity).By similarity

Protein-protein interaction databases

BioGridi278639. 4 interactors.
MINTiMINT-4669805.

Structurei

3D structure databases

ProteinModelPortaliO14018.
SMRiO14018.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 240Serine bindingBy similarity3

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000035937.
InParanoidiO14018.
KOiK01875.
OMAiANYEMEN.
OrthoDBiEOG092C513S.
PhylomeDBiO14018.

Family and domain databases

CDDicd00770. SerRS_core. 1 hit.
Gene3Di1.10.287.40. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR033729. SerRS_core.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14018-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDINLFQVE KGGNPEIIRE SQRKRGADVG VVDKVIEMYK EWVSLRFELD
60 70 80 90 100
NTNKSINRVQ KEIGLKMKAK EDASELLEEK NSLTERKKNL IEQETAKNKE
110 120 130 140 150
MLNVVSSIGN IVHDSVPVSM DEDNNEIIRK WAPEGVTVEK KNCLSHHEVL
160 170 180 190 200
TRLDGYDPER GVKVSGHRGY FLRQYGVFFN LALIQYGLDF LEKRGYIALQ
210 220 230 240 250
APTMLNKDVM AKTAQLEQFD EELYKVIDGD EERYLIATSE QPISAYHSGE
260 270 280 290 300
WFEKPSEQLP LKYAGYSTCY RREAGSHGRD AWGIFRVHAF EKIEQFVLTD
310 320 330 340 350
PEKSWEAFTE MINHAEDFYK SLELPYRIVA IVSGALNNAA AKKYDLEAWF
360 370 380 390 400
PFQGEYKELV SCSNCTDYQS RNLEIRCGVK KMGDREKKYV HCLNSTLCAT
410 420 430 440 450
ERALCCILEN YQTPDGVNVP KVLQPYMGGK TFLPFTKELP KNSTSKKGKN
Length:450
Mass (Da):51,643
Last modified:January 1, 1998 - v1
Checksum:i052DE1D4DF7EA646
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB10149.1.
PIRiT38474.
RefSeqiNP_594867.1. NM_001020296.2.

Genome annotation databases

EnsemblFungiiSPAC29A4.15.1; SPAC29A4.15.1:pep; SPAC29A4.15.
GeneIDi2542163.
KEGGispo:SPAC29A4.15.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB10149.1.
PIRiT38474.
RefSeqiNP_594867.1. NM_001020296.2.

3D structure databases

ProteinModelPortaliO14018.
SMRiO14018.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278639. 4 interactors.
MINTiMINT-4669805.

Proteomic databases

MaxQBiO14018.
PRIDEiO14018.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC29A4.15.1; SPAC29A4.15.1:pep; SPAC29A4.15.
GeneIDi2542163.
KEGGispo:SPAC29A4.15.

Organism-specific databases

EuPathDBiFungiDB:SPAC29A4.15.
PomBaseiSPAC29A4.15.

Phylogenomic databases

HOGENOMiHOG000035937.
InParanoidiO14018.
KOiK01875.
OMAiANYEMEN.
OrthoDBiEOG092C513S.
PhylomeDBiO14018.

Enzyme and pathway databases

UniPathwayiUPA00906; UER00895.

Miscellaneous databases

PROiO14018.

Family and domain databases

CDDicd00770. SerRS_core. 1 hit.
Gene3Di1.10.287.40. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR033729. SerRS_core.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYSC_SCHPO
AccessioniPrimary (citable) accession number: O14018
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.