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Protein

H/ACA ribonucleoprotein complex subunit 4

Gene

cbf5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. May function as a pseudouridine synthase (By similarity).By similarity

Catalytic activityi

RNA uridine = RNA pseudouridine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei100 – 1001NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-SPO-171319. Telomere Extension By Telomerase.

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit 4 (EC:5.4.99.-)
Alternative name(s):
Centromere-binding factor 5 homolog
Gene namesi
Name:cbf5
ORF Names:SPAC29A4.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC29A4.04c.
PomBaseiSPAC29A4.04c. cbf5.

Subcellular locationi

GO - Cellular componenti

  • box H/ACA snoRNP complex Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474H/ACA ribonucleoprotein complex subunit 4PRO_0000121981Add
BLAST

Proteomic databases

MaxQBiO14007.

Interactioni

Subunit structurei

Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs).By similarity

Protein-protein interaction databases

BioGridi278941. 7 interactions.
MINTiMINT-4669678.

Structurei

3D structure databases

ProteinModelPortaliO14007.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini271 – 34676PUAPROSITE-ProRule annotationAdd
BLAST
Repeati443 – 44531
Repeati450 – 45232
Repeati454 – 45633
Repeati457 – 45934
Repeati460 – 46235
Repeati463 – 46536
Repeati466 – 46837

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 468357 X 3 AA approximate tandem repeats of K-K-EAdd
BLAST

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated
Contains 1 PUA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000231224.
InParanoidiO14007.
KOiK11131.
OMAiETHCEAG.
OrthoDBiEOG7QNVWW.
PhylomeDBiO14007.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR032819. TruB_C.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTHPGVDF MIKPEATSAS KIDTAEWPLL LKNFDKLLVR TGHYTPIPCG
60 70 80 90 100
NNPLKRPIAE YVSSGVINLD KPANPSSHEV VAWVKKILRV EKTGHSGTLD
110 120 130 140 150
PKVTGCLIIC NDRATRLVKS QQSAGKEYVC VLRLHDSVEG ERNVASAIET
160 170 180 190 200
LTGALFQRPP LISAVKRQLR IRSIYESKLI EFDNERNLAV FWASCEAGTY
210 220 230 240 250
MRTLCVHLGL LLGVGGHMQE LRRVRSGCLS ENDDIVTMHD VLDAQWIYDN
260 270 280 290 300
TRDESYLRRV IRPLESLLVG YKRIVVKDSA VNAICYGAKL MIPGLLRYEA
310 320 330 340 350
GIEVNEEIVL ITTKGEAIAV GIAQMSTVEL STCDHGVVAK VKRCIMERDV
360 370 380 390 400
YPRRWGLGPQ SMKKKTLKKE GKLDKYGRPN ENTPADWSKS YIDYSDPNAE
410 420 430 440 450
VAKPAPVVAP AAPTVEAEVN GVEDSKKRKS VESSEKDEDE AAKKEEKRRK
460 470
KEAKKEKKEK KEKKEKKEKK KKSE
Length:474
Mass (Da):53,110
Last modified:January 1, 1998 - v1
Checksum:iB8C9896C5FAEEB71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB10131.1.
PIRiT38485.
RefSeqiNP_594878.1. NM_001020307.2.

Genome annotation databases

EnsemblFungiiSPAC29A4.04c.1; SPAC29A4.04c.1:pep; SPAC29A4.04c.
GeneIDi2542481.
KEGGispo:SPAC29A4.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB10131.1.
PIRiT38485.
RefSeqiNP_594878.1. NM_001020307.2.

3D structure databases

ProteinModelPortaliO14007.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278941. 7 interactions.
MINTiMINT-4669678.

Proteomic databases

MaxQBiO14007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC29A4.04c.1; SPAC29A4.04c.1:pep; SPAC29A4.04c.
GeneIDi2542481.
KEGGispo:SPAC29A4.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAC29A4.04c.
PomBaseiSPAC29A4.04c. cbf5.

Phylogenomic databases

HOGENOMiHOG000231224.
InParanoidiO14007.
KOiK11131.
OMAiETHCEAG.
OrthoDBiEOG7QNVWW.
PhylomeDBiO14007.

Enzyme and pathway databases

ReactomeiR-SPO-171319. Telomere Extension By Telomerase.

Miscellaneous databases

NextBioi20803536.
PROiO14007.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR032819. TruB_C.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiCBF5_SCHPO
AccessioniPrimary (citable) accession number: O14007
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.