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Protein

Replication factor C subunit 3

Gene

rfc3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. Subunit 3 binds ATP. Also involved in replication and DNA damage checkpoint controls, probably functioning as a checkpoint sensor.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi63 – 70ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • DNA strand elongation involved in DNA replication Source: PomBase
  • mitotic DNA replication leading strand elongation Source: PomBase
  • UV-damage excision repair Source: PomBase
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-SPO-110320. Translesion Synthesis by POLH.
R-SPO-174411. Polymerase switching on the C-strand of the telomere.
R-SPO-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.
R-SPO-5656169. Termination of translesion DNA synthesis.
R-SPO-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-SPO-5696400. Dual Incision in GG-NER.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-69091. Polymerase switching.

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 3
Short name:
Replication factor C3
Gene namesi
Name:rfc3
ORF Names:SPAC27E2.10c, SPAPJ698.01c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC27E2.10c.
PomBaseiSPAC27E2.10c. rfc3.

Subcellular locationi

GO - Cellular componenti

  • Ctf18 RFC-like complex Source: PomBase
  • cytosol Source: PomBase
  • DNA replication factor C complex Source: GO_Central
  • Elg1 RFC-like complex Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear DNA replication factor C complex Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
  • Rad17 RFC-like complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi216R → W: Defective DNA replication. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217551 – 342Replication factor C subunit 3Add BLAST342

Proteomic databases

MaxQBiO14003.
PRIDEiO14003.

Interactioni

Subunit structurei

Heteropentamer of subunits rfc1, rfc2, rfc3, rfc4 and rfc5 that forms a complex (RFC) with PCNA in the presence of ATP. Two other complexes exist where rfc1 can be replaced by either ctf18 or elg1 to form the ctf18-RFC or the elg1-RFC complexes respectively.1 Publication

Protein-protein interaction databases

BioGridi277995. 8 interactors.
MINTiMINT-4669645.

Structurei

3D structure databases

ProteinModelPortaliO14003.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the activator 1 small subunits family.Curated

Phylogenomic databases

HOGENOMiHOG000224152.
InParanoidiO14003.
KOiK10756.
OMAiSHKLNPA.
OrthoDBiEOG092C39MN.
PhylomeDBiO14003.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

O14003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEKGKGRA MDIDLPLGSE STLPWVEKYR PANLEDVVSH KDIISTLEKF
60 70 80 90 100
ISSNRVPHML FYGPPGTGKT STILACARKI YGPNYRNQLM ELNASDDRGI
110 120 130 140 150
DAVREQIKNF ASTRQIFAST FKMIILDEAD AMTLAAQNAL RRVIEKYTKN
160 170 180 190 200
VRFCIICNYI NKISPAIQSR CTRFRFQPLP PKEIEKTVDH VIQSEHCNID
210 220 230 240 250
PDAKMAVLRL SKGDMRKALN ILQACHAAYD HIDVSAIYNC VGHPHPSDID
260 270 280 290 300
YFLKSIMNDE FVIAFNTISS IKQQKGLALQ DILTCIFEAL DELEIKPNAK
310 320 330 340
IFILDQLATI EHRMSFGCSE KIQLSAMIAS IKTGVDLAAK VN
Length:342
Mass (Da):38,440
Last modified:May 1, 1999 - v2
Checksum:iFA179E0C9F3C6F96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017039 Genomic DNA. Translation: BAA82745.1.
AB017040 mRNA. Translation: BAA82746.1.
AJ012839 Genomic DNA. Translation: CAB38106.1.
CU329670 Genomic DNA. Translation: CAB39134.1.
PIRiT43410.
RefSeqiXP_001713099.1. XM_001713047.2.

Genome annotation databases

EnsemblFungiiSPAC27E2.10c.1; SPAC27E2.10c.1:pep; SPAC27E2.10c.
GeneIDi2541493.
KEGGispo:SPAC27E2.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017039 Genomic DNA. Translation: BAA82745.1.
AB017040 mRNA. Translation: BAA82746.1.
AJ012839 Genomic DNA. Translation: CAB38106.1.
CU329670 Genomic DNA. Translation: CAB39134.1.
PIRiT43410.
RefSeqiXP_001713099.1. XM_001713047.2.

3D structure databases

ProteinModelPortaliO14003.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277995. 8 interactors.
MINTiMINT-4669645.

Proteomic databases

MaxQBiO14003.
PRIDEiO14003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC27E2.10c.1; SPAC27E2.10c.1:pep; SPAC27E2.10c.
GeneIDi2541493.
KEGGispo:SPAC27E2.10c.

Organism-specific databases

EuPathDBiFungiDB:SPAC27E2.10c.
PomBaseiSPAC27E2.10c. rfc3.

Phylogenomic databases

HOGENOMiHOG000224152.
InParanoidiO14003.
KOiK10756.
OMAiSHKLNPA.
OrthoDBiEOG092C39MN.
PhylomeDBiO14003.

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-SPO-110320. Translesion Synthesis by POLH.
R-SPO-174411. Polymerase switching on the C-strand of the telomere.
R-SPO-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.
R-SPO-5656169. Termination of translesion DNA synthesis.
R-SPO-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-SPO-5696400. Dual Incision in GG-NER.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-69091. Polymerase switching.

Miscellaneous databases

PROiO14003.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFC3_SCHPO
AccessioniPrimary (citable) accession number: O14003
Secondary accession number(s): Q9P547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.