Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peroxide stress-activated histidine kinase mak2

Gene

mak2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the control of the SAPK-dependent transcriptional response to peroxide stress. Regulates sty1 activity.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: InterPro
  • protein kinase activity Source: PomBase

GO - Biological processi

  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • negative regulation of induction of conjugation with cellular fusion Source: PomBase
  • phosphorelay signal transduction system Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxide stress-activated histidine kinase mak2 (EC:2.7.13.3)
Alternative name(s):
His-Asp phosphorelay kinase phk1
Mcs4-associated kinase 2
Gene namesi
Name:mak2
Synonyms:phk1
ORF Names:SPAC27E2.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC27E2.09.
PomBaseiSPAC27E2.09. mak2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000814091 – 2310Peroxide stress-activated histidine kinase mak2Add BLAST2310

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1763Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1
Modified residuei22324-aspartylphosphatePROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO14002.
PRIDEiO14002.

Interactioni

Protein-protein interaction databases

BioGridi278522. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliO14002.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 292Protein kinasePROSITE-ProRule annotationAdd BLAST281
Domaini1450 – 1592GAFAdd BLAST143
Domaini1760 – 1986Histidine kinasePROSITE-ProRule annotationAdd BLAST227
Domaini2180 – 2303Response regulatoryPROSITE-ProRule annotationAdd BLAST124

Sequence similaritiesi

Contains 1 GAF domain.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiO14002.
OMAiLKTMCDV.
OrthoDBiEOG092C01M9.
PhylomeDBiO14002.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.25.40.10. 2 hits.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011006. CheY-like_superfamily.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR011009. Kinase-like_dom.
IPR027417. P-loop_NTPase.
IPR000014. PAS.
IPR000719. Prot_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00069. Pkinase. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
SM00448. REC. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF48452. SSF48452. 2 hits.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF55781. SSF55781. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O14002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLYKSLDVA IDYAISQLGE FQFQPIRTQS NPSSLLSACL VRAVHVETRR
60 70 80 90 100
KVIFKFSQQT FKLENEYFLL RQLSSHPNGR NYAIAPAYIL LLNETLGALI
110 120 130 140 150
YDDPGPNILD EWLGNPNPLD LKLFLKFALG VSYVLCFLHE KKIVHGEIRL
160 170 180 190 200
DTFHYDLNAP IHAKLLTIGS SVSPIRFTLS SLNWKRLYQV QNICHKLQFF
210 220 230 240 250
SPEQIGNVGR PLDSRSDIYS LGILFYVILT KQYPWGGQSM RIVQSIHMRQ
260 270 280 290 300
FPSVLPRRPD AFPALDQLIQ KMTAKSMNSR ISSATDLCYT IVELMQEFST
310 320 330 340 350
ITSSPLLDQK LLSINKPQQE KLKFPKLLLT NSSDYVRIFH ELVAFSSKRD
360 370 380 390 400
LLTSAKRVDK LPKQHLFKYR PVDNEATYCQ VVTVTGEKGS GKSNLLNAVA
410 420 430 440 450
DEARKFGYFA MSSFKGHHFS PYSAIFKCVS LIMQQTLREE KQLVTDYFTS
460 470 480 490 500
LWEFLGFQLI YMGELFEYVP ELNSLLSPKY NLHCKRENYF KLKKRDPQQF
510 520 530 540 550
RSASGRLGFM VCLLEILSFT SRVRPVIIIL DELHLADHPS LSLIIGMISH
560 570 580 590 600
RLPILLILAW DEPVMFKDFS KCLHEAPYAM VTDIRMNLFD RKNITEFLDS
610 620 630 640 650
TLESPTQALG PLVLLMQKLS KGNPLVLKSL LLIAFANNGF AFHPKSSSWT
660 670 680 690 700
YDLPVINRSF EALSSYDIPP LLASLLDALL PARCIEFLLW AALLVEPFPF
710 720 730 740 750
ELLRLITTSM HLFIPKEEIL DFPLNVLQFD NDNESCQFSE TFFREGILSK
760 770 780 790 800
ISLRRAESMH AQIAKELITG TAKEFYDIRT VHHILKGLGV IKKFDNTKPY
810 820 830 840 850
ILALKESADA LMQFGSYEYA TELLKSCLFL LPRNFWNSKL YTRKDLISIH
860 870 880 890 900
ISLAMCYWWS KDHENAIKVL KNPKLSSSNV YDYLPAFRLL TKIEYYKYQS
910 920 930 940 950
LRSIDKAQEL LSNLGLKLKE PTDDVLREFY DRLSTKFLEC DFLVKQSEPL
960 970 980 990 1000
DRKRIDAISV ILSECGFVLF NFSQPYYYYF SFLLAEMYLR YGNPSLRYSV
1010 1020 1030 1040 1050
MFLASYCFVT RRKPEFLLRI SQVDSDLFVI KDRSAVAHAE LIYWGLKREL
1060 1070 1080 1090 1100
CSTETGSAVT LESILLQCVM FGDKIYGAYC LACLMAQRVF RGDHIHQLLL
1110 1120 1130 1140 1150
DQENSETLLL LWDCEPPFTY YLMLIRNSLL ALFGLTNNDD PNNILTTKQR
1160 1170 1180 1190 1200
TQKDLHDKLT SKKVPCTFCC WYYAGIIFLN TLFHHYEYVM SIAQEVRKLV
1210 1220 1230 1240 1250
DGKLYERYYL ITRSFIGVAA LQLLFYKKNI SEFEREKVED VAHWAQSSLS
1260 1270 1280 1290 1300
EMAKCFHAEL YKLWVCLLEG LRQRNLGNYM EALRLFEKVT SMGASVFSPI
1310 1320 1330 1340 1350
EFPFVLELIG EFYYGRGHKF LAKSYITRAL SCLKNIGCYG VENKLRSRYS
1360 1370 1380 1390 1400
DLISDVESRG TTVVSIATTT GDYAEKLKLL RNQDINDFSL GLASYSDIFD
1410 1420 1430 1440 1450
KPLVTLPVKK SSAVDESEND FYDRNDEESF DIVSLVSVIK CGQLLSSKLR
1460 1470 1480 1490 1500
LGPLLTTVIK LVIEYSQAKH AAIILKDASN YTLAAHGNVE KAESFEPPVI
1510 1520 1530 1540 1550
LSQSDVKIPD SLLSEVFDHC RIVSLYTVSA SQDAELLRWL QEEHDMDFFA
1560 1570 1580 1590 1600
IIPLQFKESV IGALYLCLSR RAIRTGNVTF LKLLSQQIAI SVSNALLFQS
1610 1620 1630 1640 1650
LRRTITDNVT LIELQRLSYQ RYKAIEEKCI TLLDSLPCIV WTLDSDIGEI
1660 1670 1680 1690 1700
EYTNASKRNY FGVPEDCHDS LSWKTFIHPD HHHQFQEKLL NLKTLELGDI
1710 1720 1730 1740 1750
ELLLRMEDGN YHWHLCRGLS FKEDANAKKW IVVCIDINDE KEAREAAMHA
1760 1770 1780 1790 1800
VNLKTNFLAN MSHELRTPFS SFYGMLSLLS DTKLNEEQYD IVSTAKQSCT
1810 1820 1830 1840 1850
SLVQIIDDLL NFSELKSGKM KLEPDKVFDV EENIADCIEL VYPSLSSKPV
1860 1870 1880 1890 1900
QISYDIYPNV PALLAGDSAK LRQVITNLLG NSVKFTTEGH ILLRCMAIDE
1910 1920 1930 1940 1950
EINAEENQCK LRFEIEDTGI GLKEEQLKLL FNPFTQVDGS TTRIYGGSGL
1960 1970 1980 1990 2000
GLSICLQICK IMDGDIGVQS VYGEGSTFWF HVQLRNVTSK LSQKHFEESH
2010 2020 2030 2040 2050
ERFANIRQSL KNAKILVVKS FTTSRSIFRS LFSLAVVDTT TIYSDIEQQL
2060 2070 2080 2090 2100
IDSLDKRQPY DFLCIEAASG QTEQIITQIL SNQKLNKVLL IVLLPSIQRT
2110 2120 2130 2140 2150
KVRSDGDPFI TSLNKNQSRI FCFREPIRIS KLLQNFPALL SKWSTPTKLV
2160 2170 2180 2190 2200
EPSQFRASPR KVDQAVVLSS EEKEILQKKY ALIAEDNLIA RKLLTKQLSN
2210 2220 2230 2240 2250
LGFQVHAAVD GVELVKMYEA KQFGFYSVIF ADYHMPIRDG AEAVMDIRAY
2260 2270 2280 2290 2300
ERENNCSTPI PVIALTADIQ KSAKQRCLEV GMNFYLTKPF TQKQLVNAVR
2310
EFVLLEKSAR
Length:2,310
Mass (Da):264,622
Last modified:January 1, 1998 - v1
Checksum:i5A49120DB37E299D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11683.1.
PIRiT38457.
RefSeqiNP_594410.1. NM_001019841.2.

Genome annotation databases

EnsemblFungiiSPAC27E2.09.1; SPAC27E2.09.1:pep; SPAC27E2.09.
GeneIDi2542041.
KEGGispo:SPAC27E2.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11683.1.
PIRiT38457.
RefSeqiNP_594410.1. NM_001019841.2.

3D structure databases

ProteinModelPortaliO14002.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278522. 3 interactors.

Proteomic databases

MaxQBiO14002.
PRIDEiO14002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC27E2.09.1; SPAC27E2.09.1:pep; SPAC27E2.09.
GeneIDi2542041.
KEGGispo:SPAC27E2.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC27E2.09.
PomBaseiSPAC27E2.09. mak2.

Phylogenomic databases

InParanoidiO14002.
OMAiLKTMCDV.
OrthoDBiEOG092C01M9.
PhylomeDBiO14002.

Miscellaneous databases

PROiO14002.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.25.40.10. 2 hits.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011006. CheY-like_superfamily.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR011009. Kinase-like_dom.
IPR027417. P-loop_NTPase.
IPR000014. PAS.
IPR000719. Prot_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00069. Pkinase. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00065. GAF. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
SM00448. REC. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF48452. SSF48452. 2 hits.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF55781. SSF55781. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAK2_SCHPO
AccessioniPrimary (citable) accession number: O14002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.