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Protein

Probable methionine--tRNA ligase, mitochondrial

Gene

SPAC27E2.06c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei329 – 3291ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methionine--tRNA ligase, mitochondrial (EC:6.1.1.10)
Alternative name(s):
Mitochondrial methionyl-tRNA synthetase
Short name:
MtMetRS
Gene namesi
ORF Names:SPAC27E2.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC27E2.06c.
PomBaseiSPAC27E2.06c.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 539Probable methionine--tRNA ligase, mitochondrialPRO_0000374024
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiO14000.
PRIDEiO14000.

Interactioni

Protein-protein interaction databases

MINTiMINT-4669624.

Structurei

3D structure databases

ProteinModelPortaliO14000.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi28 – 3811"HIGH" regionBy similarityAdd
BLAST
Motifi326 – 3305"KMSKS" regionBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000200401.
InParanoidiO14000.
KOiK01874.
OMAiTYPAFCT.
OrthoDBiEOG7647BG.
PhylomeDBiO14000.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
InterProiIPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O14000-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRKGICRLI HQVSESSKKP YFLTTPIFYV NAAPHLGHLY SLVLTDAIAR
60 70 80 90 100
FQNLKPDVSV ISSTGTDEHG LKVQTVAQTE GVSPLQLCDR NSKRFADLAV
110 120 130 140 150
AANTKFTHFI RTTNPKHQAS VQEFWKTIQK AGMISFERHE GWYCVSDETF
160 170 180 190 200
YPESAIQKVV DPATKQEKRV SMETGKEVQW SSEMNYHFLL SKFQSRLIEH
210 220 230 240 250
YNKNPNFVQP SIFHTQVLEE LKTGISDLSI SRPKQRLSWG IPVPGNSQQT
260 270 280 290 300
IYVWLDALIN YISVIGYPWL NEKSSLSAGW PANMHVIGKD IIRFHCIYWP
310 320 330 340 350
AFLMAAGLPL PEKILVHSHW TMNKVKMSKS LGNVVDPFWL IEKYGVDTIR
360 370 380 390 400
YYLLKRGRLT SDSNFDIEEL EKDEEHDLRR SLGVLLSRLQ SKKLFISNEI
410 420 430 440 450
QKQWHKKDDF TEYEDIVHEL IELPVVCAQS IDGGCVYEVI NLVQSVLRRV
460 470 480 490 500
TKLFQLKEPW KLSDDSQEKI DTLMLVAHSL RISGILLQPI MPTKSTELLD
510 520 530
QLGIPKNQRS LQNATNVFEP TEFTFHSGNN SHLFDKRTQ
Length:539
Mass (Da):61,946
Last modified:January 1, 1998 - v1
Checksum:iAC460BB07E65D463
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11680.1.
PIRiT38454.
RefSeqiNP_594407.1. NM_001019838.2.

Genome annotation databases

EnsemblFungiiSPAC27E2.06c.1; SPAC27E2.06c.1:pep; SPAC27E2.06c.
GeneIDi2541577.
KEGGispo:SPAC27E2.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11680.1.
PIRiT38454.
RefSeqiNP_594407.1. NM_001019838.2.

3D structure databases

ProteinModelPortaliO14000.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4669624.

Proteomic databases

MaxQBiO14000.
PRIDEiO14000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC27E2.06c.1; SPAC27E2.06c.1:pep; SPAC27E2.06c.
GeneIDi2541577.
KEGGispo:SPAC27E2.06c.

Organism-specific databases

EuPathDBiFungiDB:SPAC27E2.06c.
PomBaseiSPAC27E2.06c.

Phylogenomic databases

HOGENOMiHOG000200401.
InParanoidiO14000.
KOiK01874.
OMAiTYPAFCT.
OrthoDBiEOG7647BG.
PhylomeDBiO14000.

Miscellaneous databases

PROiO14000.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
InterProiIPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYMM_SCHPO
AccessioniPrimary (citable) accession number: O14000
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.