Reviewed, UniProtKB/Swiss-Prot O14000 (SYMM_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable methionyl-tRNA synthetase, mitochondrial
    EC=6.1.1.10
Alternative name(s):
    Mitochondrial methionine--tRNA ligase
      Short name=MtMetRS

Gene names

ORF Names:

SPAC27E2.06c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Mitochondrion matrix. Ref.2
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 539		Probable methionyl-tRNA synthetase, mitochondrial		PRO_0000374024
Regions
	Motif	28 – 38	11	"HIGH" region By similarity
	Motif	326 – 330	5	"KMSKS" region By similarity
Sites
	Binding site	329	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O14000-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: AC460BB07E65D463	FASTA	539	61,946
	10 20 30 40 50 60 MLRKGICRLI HQVSESSKKP YFLTTPIFYV NAAPHLGHLY SLVLTDAIAR FQNLKPDVSV 70 80 90 100 110 120 ISSTGTDEHG LKVQTVAQTE GVSPLQLCDR NSKRFADLAV AANTKFTHFI RTTNPKHQAS 130 140 150 160 170 180 VQEFWKTIQK AGMISFERHE GWYCVSDETF YPESAIQKVV DPATKQEKRV SMETGKEVQW 190 200 210 220 230 240 SSEMNYHFLL SKFQSRLIEH YNKNPNFVQP SIFHTQVLEE LKTGISDLSI SRPKQRLSWG 250 260 270 280 290 300 IPVPGNSQQT IYVWLDALIN YISVIGYPWL NEKSSLSAGW PANMHVIGKD IIRFHCIYWP 310 320 330 340 350 360 AFLMAAGLPL PEKILVHSHW TMNKVKMSKS LGNVVDPFWL IEKYGVDTIR YYLLKRGRLT 370 380 390 400 410 420 SDSNFDIEEL EKDEEHDLRR SLGVLLSRLQ SKKLFISNEI QKQWHKKDDF TEYEDIVHEL 430 440 450 460 470 480 IELPVVCAQS IDGGCVYEVI NLVQSVLRRV TKLFQLKEPW KLSDDSQEKI DTLMLVAHSL 490 500 510 520 530 RISGILLQPI MPTKSTELLD QLGIPKNQRS LQNATNVFEP TEFTFHSGNN SHLFDKRTQ

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB11680.1.
PIR	T38454.
RefSeq	NP_594407.1.
3D structure databases
HSSP	HSSP built from PDB template 1A8H based on UniProtKB P23395.
ModBase	Search...
Protein-protein interaction databases
STRING	O14000.
Genome annotation databases
GeneID	2541577.
KEGG	spo:SPAC27E2.06c.
NMPDR	fig|4896.1.peg.4377.
Organism-specific databases
GeneDB_Spombe	SPAC27E2.06c.
Phylogenomic databases
OMA	TRFIRTT.
Gene expression databases
ArrayExpress	O14000.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SYMM_SCHPO

Accession

Primary (citable) accession number: O14000

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)