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Protein

Obg-like ATPase 1

Gene

SPAC27E2.03c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP (By similarity). Negatively regulates the G2/M transition in the cell cycle.1 PublicationUniRule annotation

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341MagnesiumBy similarity
Metal bindingi55 – 551MagnesiumBy similarity
Binding sitei233 – 2331ATP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 356ATPUniRule annotation

GO - Molecular functioni

  1. ATPase activity Source: PomBase
  2. ATP binding Source: UniProtKB-HAMAP
  3. GTP binding Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. ribosomal large subunit binding Source: UniProtKB-HAMAP
  6. ribosome binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Obg-like ATPase 1UniRule annotation
Gene namesi
ORF Names:SPAC27E2.03c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC27E2.03c.

Subcellular locationi

Cytoplasm 1 PublicationUniRule annotation. Nucleus 1 PublicationUniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Obg-like ATPase 1PRO_0000356254Add
BLAST

Proteomic databases

MaxQBiO13998.
PaxDbiO13998.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

BioGridi277939. 11 interactions.
MINTiMINT-4669602.
STRINGi4896.SPAC27E2.03c-1.

Structurei

Secondary structure

1
392
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 195Combined sources
Beta strandi22 – 276Combined sources
Beta strandi29 – 324Combined sources
Helixi33 – 4210Combined sources
Turni44 – 463Combined sources
Beta strandi60 – 656Combined sources
Helixi68 – 7710Combined sources
Beta strandi80 – 834Combined sources
Beta strandi85 – 906Combined sources
Helixi93 – 953Combined sources
Beta strandi101 – 1055Combined sources
Helixi107 – 1137Combined sources
Beta strandi117 – 1248Combined sources
Beta strandi135 – 1384Combined sources
Helixi140 – 16829Combined sources
Beta strandi176 – 1783Combined sources
Helixi179 – 19719Combined sources
Helixi203 – 2053Combined sources
Helixi210 – 2178Combined sources
Helixi222 – 2243Combined sources
Beta strandi227 – 2326Combined sources
Helixi235 – 2384Combined sources
Turni239 – 2413Combined sources
Helixi246 – 2549Combined sources
Beta strandi262 – 2654Combined sources
Helixi267 – 2737Combined sources
Helixi278 – 28710Combined sources
Helixi294 – 30411Combined sources
Beta strandi307 – 3126Combined sources
Beta strandi315 – 32410Combined sources
Helixi329 – 3368Combined sources
Helixi338 – 3425Combined sources
Beta strandi345 – 3506Combined sources
Helixi352 – 3587Combined sources
Helixi361 – 3666Combined sources
Beta strandi372 – 3743Combined sources
Beta strandi384 – 3863Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NI3X-ray2.80A1-392[»]
ProteinModelPortaliO13998.
SMRiO13998. Positions 11-388.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO13998.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 285265OBG-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0012.
HOGENOMiHOG000087628.
InParanoidiO13998.
KOiK06942.
OMAiRFDWLVE.
OrthoDBiEOG7XPZG2.
PhylomeDBiO13998.

Family and domain databases

Gene3Di1.10.150.300. 1 hit.
3.10.20.30. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00944. YchF_OLA1_ATPase.
InterProiIPR004396. ATPase_YchF/OLA1.
IPR012675. Beta-grasp_dom.
IPR013029. DUF933.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
IPR012676. TGS-like.
IPR023192. TGS-like_dom.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF06071. YchF-GTPase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006641. CHP00092. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00092. TIGR00092. 1 hit.
PROSITEiPS51710. G_OBG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13998-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPKKQQEVV KVQWGRPGNN LKTGIVGMPN VGKSTFFRAI TKSVLGNPAN
60 70 80 90 100
YPYATIDPEE AKVAVPDERF DWLCEAYKPK SRVPAFLTVF DIAGLTKGAS
110 120 130 140 150
TGVGLGNAFL SHVRAVDAIY QVVRAFDDAE IIHVEGDVDP IRDLSIIVDE
160 170 180 190 200
LLIKDAEFVE KHLEGLRKIT SRGANTLEMK AKKEEQAIIE KVYQYLTETK
210 220 230 240 250
QPIRKGDWSN REVEIINSLY LLTAKPVIYL VNMSERDFLR QKNKYLPKIK
260 270 280 290 300
KWIDENSPGD TLIPMSVAFE ERLTNFTEEE AIEECKKLNT KSMLPKIIVT
310 320 330 340 350
GYNALNLINY FTCGEDEVRS WTIRKGTKAP QAAGVIHTDF EKAFVVGEIM
360 370 380 390
HYQDLFDYKT ENACRAAGKY LTKGKEYVME SGDIAHWKAG KR
Length:392
Mass (Da):44,333
Last modified:January 1, 1998 - v1
Checksum:iAEBBAF47DA1BC5F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11677.1.
PIRiT38450.
RefSeqiNP_594403.1. NM_001019834.2.

Genome annotation databases

EnsemblFungiiSPAC27E2.03c.1; SPAC27E2.03c.1:pep; SPAC27E2.03c.
GeneIDi2541434.
KEGGispo:SPAC27E2.03c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11677.1.
PIRiT38450.
RefSeqiNP_594403.1. NM_001019834.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NI3X-ray2.80A1-392[»]
ProteinModelPortaliO13998.
SMRiO13998. Positions 11-388.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277939. 11 interactions.
MINTiMINT-4669602.
STRINGi4896.SPAC27E2.03c-1.

Proteomic databases

MaxQBiO13998.
PaxDbiO13998.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC27E2.03c.1; SPAC27E2.03c.1:pep; SPAC27E2.03c.
GeneIDi2541434.
KEGGispo:SPAC27E2.03c.

Organism-specific databases

PomBaseiSPAC27E2.03c.

Phylogenomic databases

eggNOGiCOG0012.
HOGENOMiHOG000087628.
InParanoidiO13998.
KOiK06942.
OMAiRFDWLVE.
OrthoDBiEOG7XPZG2.
PhylomeDBiO13998.

Miscellaneous databases

EvolutionaryTraceiO13998.
NextBioi20802536.
PROiO13998.

Family and domain databases

Gene3Di1.10.150.300. 1 hit.
3.10.20.30. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00944. YchF_OLA1_ATPase.
InterProiIPR004396. ATPase_YchF/OLA1.
IPR012675. Beta-grasp_dom.
IPR013029. DUF933.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
IPR012676. TGS-like.
IPR023192. TGS-like_dom.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF06071. YchF-GTPase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006641. CHP00092. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00092. TIGR00092. 1 hit.
PROSITEiPS51710. G_OBG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  3. "A systematic screen reveals new elements acting at the G2/M cell cycle control."
    Navarro F.J., Nurse P.
    Genome Biol. 13:R36.1-R36.10(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Structure of the s. pombe ychf GTP-binding protein."
    New York structural genomix research consortium (NYSGXRC)
    Submitted (JAN-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

Entry informationi

Entry nameiOLA1_SCHPO
AccessioniPrimary (citable) accession number: O13998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.