O13988 (POT1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protection of telomeres protein 1

Gene names

Name:	pot1
ORF Names:	SPAC26H5.06

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length. Ref.2 Ref.3 Ref.5
Subunit structure	Self-associates. Interacts with ccq1, poz1 and tpz1. Ref.3 Ref.5
Subcellular location	Nucleus. Chromosome › telomere Ref.4 Ref.5.
Sequence similarities	Belongs to the telombin family.

Ontologies

Keywords
Cellular component	Chromosome Nucleus Telomere
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitotic sister chromatid segregation Inferred from mutant phenotype Ref.2. Source: PomBase telomere capping Inferred from direct assay PubMed 15637058. Source: PomBase telomere maintenance via telomerase Inferred from mutant phenotype Ref.2. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase telosome Inferred by curator. Source: PomBase
Molecular_function	single-stranded telomeric DNA binding Inferred from direct assay Ref.3. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 555

555

Protection of telomeres protein 1

PRO_0000121732

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

555

Helix Strand Turn

Details...

Helix

8 – 16

9

Beta strand

18 – 22

5

Beta strand

25 – 28

4

Helix

30 – 33

4

Beta strand

41 – 57

17

Beta strand

65 – 72

8

Beta strand

78 – 80

3

Beta strand

83 – 93

11

Beta strand

104 – 115

12

Beta strand

118 – 131

14

Helix

156 – 173

18

Helix

204 – 206

3

Beta strand

212 – 224

13

Beta strand

229 – 234

6

Turn

247 – 249

3

Beta strand

258 – 260

3

Beta strand

263 – 267

5

Helix

269 – 275

7

Turn

276 – 278

3

Beta strand

284 – 294

11

Beta strand

300 – 304

5

Helix

310 – 312

3

Beta strand

315 – 319

5

Helix

324 – 326

3

Helix

327 – 333

7

Helix

334 – 336

3

Sequences

	Sequence		Length	Mass (Da)
	O13988 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: A79DAA95A0C4F803	FASTA	555	64,111
	10 20 30 40 50 60 MGEDVIDSLQ LNELLNAGEY KIGELTFQSI RSSQELQKKN TIVNLFGIVK DFTPSRQSLH 70 80 90 100 110 120 GTKDWVTTVY LWDPTCDTSS IGLQIHLFSK QGNDLPVIKQ VGQPLLLHQI TLRSYRDRTQ 130 140 150 160 170 180 GLSKDQFRYA LWPDFSSNSK DTLCPQPMPR LMKTGDKEEQ FALLLNKIWD EQTNKHKNGE 190 200 210 220 230 240 LLSTSSARQN QTGLSYPSVS FSLLSQITPH QRCSFYAQVI KTWYSDKNFT LYVTDYTENE 250 260 270 280 290 300 LFFPMSPYTS SSRWRGPFGR FSIRCILWDE HDFYCRNYIK EGDYVVMKNV RTKIDHLGYL 310 320 330 340 350 360 ECILHGDSAK RYNMSIEKVD SEEPELNEIK SRKRLYVQNC QNGIEAVIEK LSQSQQSENP 370 380 390 400 410 420 FIAHELKQTS VNEITAHVIN EPASLKLTTI STILHAPLQN LLKPRKHRLR VQVVDFWPKS 430 440 450 460 470 480 LTQFAVLSQP PSSYVWMFAL LVRDVSNVTL PVIFFDSDAA ELINSSKIQP CNLADHPQMT 490 500 510 520 530 540 LQLKERLFLI WGNLEERIQH HISKGESPTL AAEDVETPWF DIYVKEYIPV IGNTKDHQSL 550 TFLQKRWRGF GTKIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Pot1, the putative telomere end-binding protein in fission yeast and humans." Baumann P., Cech T.R. Science 292:1171-1175(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"Cooperative binding of single-stranded telomeric DNA by the Pot1 protein of Schizosaccharomyces pombe." Lei M., Baumann P., Cech T.R. Biochemistry 41:14560-14568(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[4]	"Protection of telomeres by a conserved Stn1-Ten1 complex." Martin V., Du L.-L., Rozenzhak S., Russell P. Proc. Natl. Acad. Sci. U.S.A. 104:14038-14043(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SELF-ASSOCIATION, SUBCELLULAR LOCATION.
[5]	"Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length." Miyoshi T., Kanoh J., Saito M., Ishikawa F. Science 320:1341-1344(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CCQ1; POZ1 AND TPZ1.
[6]	"DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA." Lei M., Podell E.R., Baumann P., Cech T.R. Nature 426:198-203(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 2-185, TELOMERIC SINGLE-STRANDED DNA-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CU329670 Genomic DNA. Translation: CAB16192.2.

PIR

T38425.

RefSeq

NP_594453.1. NM_001019882.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QZG	X-ray	1.90	A/B	2-185	[»]
1QZH	X-ray	2.40	A/B/C/D/E/F	2-185	[»]
4HID	X-ray	1.82	A	200-339	[»]
4HIK	X-ray	1.64	A	200-339	[»]
4HIM	X-ray	1.75	A	200-339	[»]
4HIO	X-ray	1.75	A	200-339	[»]
4HJ5	X-ray	2.04	A	200-339	[»]
4HJ7	X-ray	1.78	A	200-339	[»]
4HJ8	X-ray	2.04	A	200-339	[»]
4HJ9	X-ray	1.85	A	200-339	[»]
4HJA	X-ray	2.10	A	200-339	[»]

ProteinModelPortal

O13988.

SMR

O13988. Positions 5-174.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-38935N.

IntAct

O13988. 2 interactions.

MINT

MINT-4669519.

STRING

4896.SPAC26H5.06-1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

SPAC26H5.06.1; SPAC26H5.06.1:pep; SPAC26H5.06.

GeneID

2542703.

KEGG

spo:SPAC26H5.06.

Organism-specific databases

PomBase

SPAC26H5.06.

Phylogenomic databases

eggNOG

NOG269056.

KO

K11109.

OrthoDB

EOG4S4SQD.

Family and domain databases

Gene3D

2.40.50.140. 1 hit.

InterPro

IPR012340. NA-bd_OB-fold.
IPR011564. Telomer_end-bd.
[Graphical view]

Pfam

PF02765. Telo_bind. 1 hit.
[Graphical view]

SMART

SM00976. Telo_bind. 1 hit.
[Graphical view]

SUPFAM

SSF50249. Nucleic_acid_OB. 2 hits.

ProtoNet

Search...

Other

EvolutionaryTrace

O13988.

NextBio

20803751.

Entry information

Entry name

POT1_SCHPO

Accession

Primary (citable) accession number: O13988

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program