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Protein

ATP-dependent helicase hrq1

Gene

hrq1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Helicase with 3'-5' helicase activity involved in genome stability (PubMed:22064477). Functions in the nucleotide excision repair (NER) pathway and plays a critical role in DNA interstrand cross-link repair (PubMed:22064477). Unwinds relatively long duplex DNA up to 120-bp and requires a long 3'-tail of at least 70 nucleotides for efficient unwinding of duplex DNA (By similarity). Shows both processive helicase and DNA strand annealing activities (By similarity). Affects telomere length by a non-catalytic mechanism, probably through inhibiting telomerase by competing with it for ssDNA binding (By similarity).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Cofactori

Mg2+By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi333 – 340ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: PomBase
  • ATP-dependent RNA helicase activity Source: GO_Central
  • bubble DNA binding Source: PomBase

GO - Biological processi

  • nucleotide-excision repair Source: PomBase
  • nucleotide-excision repair involved in interstrand cross-link repair Source: PomBase
  • RNA secondary structure unwinding Source: GO_Central
  • UV-damage excision repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase hrq1Curated (EC:3.6.4.121 Publication)
Alternative name(s):
Homologous to recQ protein 11 Publication
Gene namesi
Name:hrq11 Publication
ORF Names:SPAC23A1.19cImported, SPAC26H5.01cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23A1.19c.
PomBaseiSPAC23A1.19c. hrq1.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to hypersensitivity to DNA interstrand cross-linking (ICL) agents mitomycin C (MMC) and cisplatin, in response to both chronic and acute exposures.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003538181 – 1063ATP-dependent helicase hrq1Add BLAST1063

Proteomic databases

MaxQBiO13983.
PRIDEiO13983.

Interactioni

Subunit structurei

Forms heptamer rings (By similarity). Interacts with rhp14 (PubMed:22064477).By similarity1 Publication

Protein-protein interaction databases

BioGridi278518. 160 interactors.

Structurei

3D structure databases

ProteinModelPortaliO13983.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini320 – 503Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST184
Domaini539 – 717Helicase C-terminalPROSITE-ProRule annotationAdd BLAST179

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi444 – 447DEAH box4

Sequence similaritiesi

Belongs to the helicase family. HRQ1 subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000163591.
InParanoidiO13983.
KOiK06877.
OMAiCELLMKE.
OrthoDBiEOG092C0E4A.
PhylomeDBiO13983.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014939. CDT1_Gemini-bd-like.
IPR018973. DEAD/DEAH-box_helicase.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08839. CDT1. 1 hit.
PF00270. DEAD. 1 hit.
PF09369. DUF1998. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM01075. CDT1. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13983-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTPIKKEE SNDQDDKFEF KKYINEGKLP LKADNPKKKP QLGTIQANQP
60 70 80 90 100
IPSIFDNLFN LFKVINTTYT FLYLRNSLTI TFPLLNSSVK QSLKKELTIG
110 120 130 140 150
DLSQLREICP QIIELNYKSL ASLALEINKN VYTDLNPELY TGSTVSQSSE
160 170 180 190 200
YVLVIELLET QERSSKRRRR EGPTMKANIQ RQKLDFNNLK KAIELRNQKF
210 220 230 240 250
LQGIKEYIKK CQLTELDPTQ QLLTQSRKNQ PVPPDSPSIP NDSIENCNLN
260 270 280 290 300
TKACSIEELL NEIASESSYE GQIVQEALHT YPAVEAQYGA LSRPLSQELI
310 320 330 340 350
NALYTSRNIE KTYKHQADAI NHLWNGFHVI VSTSTSSGKS LIYQIPILQS
360 370 380 390 400
LLEDNQSTAF FVFPTKSLAQ DQKKSLIDIL SYMPTLKNIR VDTFDGDTPL
410 420 430 440 450
ESRESIIRSA NIIFTNPDML HQTILPNANR WYYFFKNLKL FVLDEAHVYN
460 470 480 490 500
GIFGVHVAFV LRRMRRIAEY FGNSQYRFVS CSATIEDPLQ HMKKIFGVDN
510 520 530 540 550
IKLINYTSSP SGSKKFVMWN PPYVDPKHPD DGKKSAISEA SKLLIKFAEK
560 570 580 590 600
RVRTIVFCRV RKTCESLMRL VRQELKTKQK GDLLSKIQSY RAGYTVQERR
610 620 630 640 650
KIESEMFNGK LYGIIATNAL ELGIDIGSLD AVITIGFPYS LSNLRQQFGR
660 670 680 690 700
AGRRNKSSLA VYIVETFPVD QFYLKHPILI HTQPNAELTL DLTNEVLLAS
710 720 730 740 750
HLQCAAYELP INIRSDEKFF GNQIQDICEA NLEMVEESYR PHPKYLPFPA
760 770 780 790 800
SQVRIRSVSE DMFTLVDVTN DKNVILELLE PFRVALTAYE GAVYVYQGKT
810 820 830 840 850
FIIRLLNINK RIITAHQVDV EWSTLQRDFT DVDPVRSLMK KTMHGSTNIY
860 870 880 890 900
FGAVKATLHV FGYFKVNKQK DILDVVDITD HPVEIDSRGF WIDVPWHIIE
910 920 930 940 950
VLSLKKINGA ASIHAAQHAL LSLMPIFISN SGNDIRTECK AGEKEYKEAK
960 970 980 990 1000
SERRRPSRLI FYDNCGDSSG AGLCNKAYEH TDELITMAIE RIESCDCKVR
1010 1020 1030 1040 1050
EGCPGCITSS KFEGGVCSGE VLDKVGALIL LKMLLCQHVN LDIYADGPEI
1060
DSYHALRTLI PSC
Length:1,063
Mass (Da):120,970
Last modified:August 21, 2007 - v4
Checksum:i2E544C415AC40B03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA16993.2.
RefSeqiNP_594448.2. NM_001019877.2.

Genome annotation databases

EnsemblFungiiSPAC23A1.19c.1; SPAC23A1.19c.1:pep; SPAC23A1.19c.
GeneIDi2542036.
KEGGispo:SPAC23A1.19c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAA16993.2.
RefSeqiNP_594448.2. NM_001019877.2.

3D structure databases

ProteinModelPortaliO13983.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278518. 160 interactors.

Proteomic databases

MaxQBiO13983.
PRIDEiO13983.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC23A1.19c.1; SPAC23A1.19c.1:pep; SPAC23A1.19c.
GeneIDi2542036.
KEGGispo:SPAC23A1.19c.

Organism-specific databases

EuPathDBiFungiDB:SPAC23A1.19c.
PomBaseiSPAC23A1.19c. hrq1.

Phylogenomic databases

HOGENOMiHOG000163591.
InParanoidiO13983.
KOiK06877.
OMAiCELLMKE.
OrthoDBiEOG092C0E4A.
PhylomeDBiO13983.

Miscellaneous databases

PROiO13983.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014939. CDT1_Gemini-bd-like.
IPR018973. DEAD/DEAH-box_helicase.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08839. CDT1. 1 hit.
PF00270. DEAD. 1 hit.
PF09369. DUF1998. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM01075. CDT1. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHRQ1_SCHPO
AccessioniPrimary (citable) accession number: O13983
Secondary accession number(s): O42856
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: August 21, 2007
Last modified: October 5, 2016
This is version 111 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.