O13974 (OTU1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative ubiquitin thioesterase otu1
EC=3.4.19.12

Alternative name(s):
Meiotically up-regulated gene 141 protein
OTU domain-containing protein 1

Gene names

Name:	otu1
Synonyms:	mug141
ORF Names:	SPAC24C9.14

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation By similarity. Has a role in meiosis. Ref.2
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm. Nucleus Ref.3.
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Meiosis Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	meiosis Inferred from electronic annotation. Source: UniProtKB-KW protein deubiquitination Inferred from direct assay PubMed 20838651. Source: PomBase proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay Ref.3. Source: PomBase nucleus Inferred from direct assay Ref.3PubMed 20838651. Source: PomBase
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-specific protease activity Inferred from direct assay PubMed 20838651. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Putative ubiquitin thioesterase otu1		PRO_0000300503
Regions
	Domain	135 – 254	120	OTU
	Zinc finger	299 – 323	25	C2H2-type
Sites
	Active site	143	1	By similarity
	Active site	146	1	Nucleophile By similarity
	Active site	323	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O13974 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 39356AC34E1C0813	FASTA	329	35,668
	10 20 30 40 50 60 MSSLRLRLKY ENQSAVETVE ANATVGSFLD LVAAKFSLPR NSIALKFGFP PQDIPLVNSD 70 80 90 100 110 120 VPLSTLVSSG QQILVLKNAA TSFSTNEPAK PPIPNAATKP TFPPQTEISN PPAVSHQSKN 130 140 150 160 170 180 TSQDPPYVST PIGDIALRVM PDDNSCLFRA LSKPLGFSPY ELREIVANQV LSNPDIYSTA 190 200 210 220 230 240 ILGKPSIEYA SWIRKETSWG GYIELSILSS HFGVEICSVD VKTGRVDSYN PQPATGQRTY 250 260 270 280 290 300 IVYSGIHYDL AALAAVLWDT DVDVVLFDAS DVTITPYVQQ LASLLKNMHY YTDTASFSIR 310 320 CTICGTGLVG EKDATAHALA TGHTQFGEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events." Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L., Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P., Smith G.R., Moreno S. Curr. Biol. 15:2056-2062(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN MEIOSIS.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB11271.1.
PIR	T38355.
RefSeq	NP_594039.1. NM_001019464.2.
3D structure databases
ProteinModelPortal	O13974.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4669457.
STRING	4896.SPAC24C9.14-1.
Protein family/group databases
MEROPS	C85.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC24C9.14.1; SPAC24C9.14.1:pep; SPAC24C9.14.
GeneID	2542649.
KEGG	spo:SPAC24C9.14.
Organism-specific databases
PomBase	SPAC24C9.14.
Phylogenomic databases
eggNOG	COG5539.
HOGENOM	HOG000193461.
KO	K13719.
OrthoDB	EOG42NN8R.
Family and domain databases
InterPro	IPR003323. OTU. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20803698.

Entry information

Entry name

OTU1_SCHPO

Accession

Primary (citable) accession number: O13974

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	January 1, 1998
Last modified:	May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program