Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O13974

- OTU1_SCHPO

UniProt

O13974 - OTU1_SCHPO

Protein

Putative ubiquitin thioesterase otu1

Gene

otu1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation By similarity. Has a role in meiosis.By similarity1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei143 – 1431By similarity
    Active sitei146 – 1461NucleophileBy similarity
    Active sitei323 – 3231By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri299 – 32325C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: PomBase
    3. ubiquitin-specific protease activity Source: PomBase

    GO - Biological processi

    1. meiotic nuclear division Source: UniProtKB-KW
    2. protein deubiquitination Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Meiosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC85.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative ubiquitin thioesterase otu1 (EC:3.4.19.12)
    Alternative name(s):
    Meiotically up-regulated gene 141 protein
    OTU domain-containing protein 1
    Gene namesi
    Name:otu1
    Synonyms:mug141
    ORF Names:SPAC24C9.14
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC24C9.14.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Putative ubiquitin thioesterase otu1PRO_0000300503Add
    BLAST

    Proteomic databases

    MaxQBiO13974.

    Interactioni

    Protein-protein interaction databases

    BioGridi279103. 18 interactions.
    MINTiMINT-4669457.
    STRINGi4896.SPAC24C9.14-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO13974.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini135 – 254120OTUPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri299 – 32325C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5539.
    HOGENOMiHOG000193461.
    KOiK13719.
    OMAiSCLFHAI.
    OrthoDBiEOG790GCB.
    PhylomeDBiO13974.

    Family and domain databases

    InterProiIPR003323. OTU.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O13974-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLRLRLKY ENQSAVETVE ANATVGSFLD LVAAKFSLPR NSIALKFGFP    50
    PQDIPLVNSD VPLSTLVSSG QQILVLKNAA TSFSTNEPAK PPIPNAATKP 100
    TFPPQTEISN PPAVSHQSKN TSQDPPYVST PIGDIALRVM PDDNSCLFRA 150
    LSKPLGFSPY ELREIVANQV LSNPDIYSTA ILGKPSIEYA SWIRKETSWG 200
    GYIELSILSS HFGVEICSVD VKTGRVDSYN PQPATGQRTY IVYSGIHYDL 250
    AALAAVLWDT DVDVVLFDAS DVTITPYVQQ LASLLKNMHY YTDTASFSIR 300
    CTICGTGLVG EKDATAHALA TGHTQFGEY 329
    Length:329
    Mass (Da):35,668
    Last modified:January 1, 1998 - v1
    Checksum:i39356AC34E1C0813
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11271.1.
    PIRiT38355.
    RefSeqiNP_594039.1. NM_001019464.2.

    Genome annotation databases

    EnsemblFungiiSPAC24C9.14.1; SPAC24C9.14.1:pep; SPAC24C9.14.
    GeneIDi2542649.
    KEGGispo:SPAC24C9.14.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11271.1 .
    PIRi T38355.
    RefSeqi NP_594039.1. NM_001019464.2.

    3D structure databases

    ProteinModelPortali O13974.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279103. 18 interactions.
    MINTi MINT-4669457.
    STRINGi 4896.SPAC24C9.14-1.

    Protein family/group databases

    MEROPSi C85.007.

    Proteomic databases

    MaxQBi O13974.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC24C9.14.1 ; SPAC24C9.14.1:pep ; SPAC24C9.14 .
    GeneIDi 2542649.
    KEGGi spo:SPAC24C9.14.

    Organism-specific databases

    PomBasei SPAC24C9.14.

    Phylogenomic databases

    eggNOGi COG5539.
    HOGENOMi HOG000193461.
    KOi K13719.
    OMAi SCLFHAI.
    OrthoDBi EOG790GCB.
    PhylomeDBi O13974.

    Miscellaneous databases

    NextBioi 20803698.
    PROi O13974.

    Family and domain databases

    InterProi IPR003323. OTU.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events."
      Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L., Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P., Smith G.R., Moreno S.
      Curr. Biol. 15:2056-2062(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MEIOSIS.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiOTU1_SCHPO
    AccessioniPrimary (citable) accession number: O13974
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3