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Protein

Aconitate hydratase, mitochondrial

Gene

SPAC24C9.06c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate, a step in the citric acid cycle.By similarity

Catalytic activityi

Citrate = isocitrate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable citrate synthase, mitochondrial (cit1)
  2. Aconitate hydratase, mitochondrial (SPAC24C9.06c)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106SubstrateBy similarity1
Metal bindingi392Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi455Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi458Iron-sulfur (4Fe-4S)By similarity1
Binding sitei481SubstrateBy similarity1
Binding sitei486SubstrateBy similarity1
Binding sitei613SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00718.

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase, mitochondrial (EC:4.2.1.3)
Short name:
Aconitase
Alternative name(s):
Citrate hydro-lyase
Gene namesi
ORF Names:SPAC24C9.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC24C9.06c.
PomBaseiSPAC24C9.06c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionSequence analysisAdd BLAST32
ChainiPRO_000000054633 – 789Aconitate hydratase, mitochondrialAdd BLAST757

Proteomic databases

MaxQBiO13966.
PRIDEiO13966.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi277955. 1 interactor.
MINTiMINT-4669404.

Structurei

3D structure databases

ProteinModelPortaliO13966.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni199 – 201Substrate bindingBy similarity3
Regioni676 – 677Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000224293.
InParanoidiO13966.
KOiK01681.
OMAiRSVVANM.
OrthoDBiEOG092C0DQS.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR006248. Aconitase_mito-like.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01340. aconitase_mito. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFCKISRAPA RMGSRIFTQS TLRSFSCAPV AANIDAKKVA MSNFEKNKFI
60 70 80 90 100
NYQRIKDNLE IVKKRLNRPL TYSEKILYGH LDDPVNQDIE RGVSYLKLRP
110 120 130 140 150
DRVACQDATA QMAILQFMSA GMPEVAVPVT VHCDHLIEAY EGGPIDLERA
160 170 180 190 200
NVTNKEVYDF LQTACAKYNI GFWRPGSGII HQIVLENYAF PGGLLIGTDS
210 220 230 240 250
HTPNAGGLGM VAIGVGGADA VDVMANLPWE LKCPKVIGVK LTGQLKGWTS
260 270 280 290 300
PKDVILKVAG ILTVKGGTGA IVEYFGPGVE SLSCTGMGTI CNMGAEIGAT
310 320 330 340 350
TSIFPFNPRM SEYLRATNRS AIADYAEEFA PIIAADENAH YDQIIEIDLN
360 370 380 390 400
TLEPHLNGPF TPDLATPISK FKEAVKKNDW PQELKVGLIG SCTNSSYEDM
410 420 430 440 450
SRAASICQQA IDKGIKTKSL FTITPGSEQV RATLTRDGQL DTMRKAGGIV
460 470 480 490 500
LANACGPCIG QWKRTDVKKG EKNSIVTSYN RNFTGRNDAN PATHAFVTSP
510 520 530 540 550
DIVTAMVFSG DMNFNPLTDT LKDKDGNDFK FEPPTGAGLP SKGYDPGSNT
560 570 580 590 600
YVAPSSVNVK DVAIDPHSKR LQRLTPFKKW DGKDMKGLKI LIKAKGKCTT
610 620 630 640 650
DHISAAGPWL KYRGHLQNIS NNYMIGAINA ENGEANKLKD QLTGEYKTVP
660 670 680 690 700
NVAIDYRDHG IRWVTLGEQN FGEGSSREHA ALEPRYLGGA AVITKSFARI
710 720 730 740 750
HETNLKKQGL LPLTFADPAA YDKISPFDTV DIDGLTTFAP GKPLTLVVHP
760 770 780
ADGSAEWSTK LNHTFNKDQI EWFKAGSALN HMANMHKQK
Length:789
Mass (Da):86,113
Last modified:April 18, 2012 - v2
Checksum:iB80A9654690BA9FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11263.2.
PIRiT38347.
RefSeqiNP_594031.2. NM_001019456.2.

Genome annotation databases

EnsemblFungiiSPAC24C9.06c.1; SPAC24C9.06c.1:pep; SPAC24C9.06c.
GeneIDi2541450.
KEGGispo:SPAC24C9.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11263.2.
PIRiT38347.
RefSeqiNP_594031.2. NM_001019456.2.

3D structure databases

ProteinModelPortaliO13966.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277955. 1 interactor.
MINTiMINT-4669404.

Proteomic databases

MaxQBiO13966.
PRIDEiO13966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC24C9.06c.1; SPAC24C9.06c.1:pep; SPAC24C9.06c.
GeneIDi2541450.
KEGGispo:SPAC24C9.06c.

Organism-specific databases

EuPathDBiFungiDB:SPAC24C9.06c.
PomBaseiSPAC24C9.06c.

Phylogenomic databases

HOGENOMiHOG000224293.
InParanoidiO13966.
KOiK01681.
OMAiRSVVANM.
OrthoDBiEOG092C0DQS.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.
ReactomeiR-SPO-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiO13966.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR006248. Aconitase_mito-like.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR01340. aconitase_mito. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACON_SCHPO
AccessioniPrimary (citable) accession number: O13966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 18, 2012
Last modified: October 5, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.