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Protein

RING-box protein pip1

Gene

pip1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of E3 ubiquitin ligase SCF complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recruit the E2 ubiquitination enzyme, like UBC3/CDC34, to the complex and brings it into close proximity to the substrate. Component of the rik1-associated E3 ubiquitin ligase complex that shows ubiquitin ligase activity and is required for histone H3K9 methylation. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting swi6/HP1 to methylated histones which leads to transcriptional silencing within centromeric heterochromatin, telomeric regions and at the silent mating-type loci.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Zinc 1By similarity1
Metal bindingi44Zinc 1By similarity1
Metal bindingi52Zinc 3By similarity1
Metal bindingi55Zinc 3By similarity1
Metal bindingi67Zinc 3By similarity1
Metal bindingi74Zinc 2By similarity1
Metal bindingi76Zinc 2By similarity1
Metal bindingi79Zinc 1By similarity1
Metal bindingi81Zinc 3By similarity1
Metal bindingi82Zinc 1By similarity1
Metal bindingi93Zinc 2By similarity1
Metal bindingi96Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri52 – 97RING-typePROSITE-ProRule annotationAdd BLAST46

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SPO-5632684 Hedgehog 'on' state
R-SPO-5696395 Formation of Incision Complex in GG-NER
R-SPO-5696400 Dual Incision in GG-NER
R-SPO-6781823 Formation of TC-NER Pre-Incision Complex
R-SPO-6782135 Dual incision in TC-NER
R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SPO-68949 Orc1 removal from chromatin
R-SPO-8951664 Neddylation
R-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
RING-box protein pip1
Short name:
RING-box protein 1
Alternative name(s):
Pop-interacting protein 1
Gene namesi
Name:pip1
ORF Names:SPAC23H4.18c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23H4.18c
PomBaseiSPAC23H4.18c

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560211 – 107RING-box protein pip1Add BLAST107

Proteomic databases

MaxQBiO13959
PaxDbiO13959
PRIDEiO13959

Interactioni

Subunit structurei

Part of a SCF E3 ubiquitin ligase complex containing psh1, pip1, pcul1 and the F-box proteins pop1 and pop2. Instead of the pop1/pop2 heterodimer also homooligomers of pop1 or pop2 may be present in the complex. Component of the rik1-associated E3 ubiquitin ligase complex composed of at least clr4, cul4, pip1, raf1 and raf2. Interacts with cul3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
skp1Q9Y7096EBI-1112060,EBI-1172248

GO - Molecular functioni

Protein-protein interaction databases

BioGridi278387, 11 interactors
IntActiO13959, 9 interactors
STRINGi4896.SPAC23H4.18c.1

Structurei

3D structure databases

ProteinModelPortaliO13959
SMRiO13959
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri52 – 97RING-typePROSITE-ProRule annotationAdd BLAST46

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000171951
InParanoidiO13959
KOiK03868
OMAiNHIMEPC
OrthoDBiEOG092C4PUH
PhylomeDBiO13959

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR024766 Znf_RING_H2
PfamiView protein in Pfam
PF12678 zf-rbx1, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

O13959-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDEMQIDKK EVEIEQKPPR FEIKKWNAVA LWQWDIVVDN CAICRNHIMD
60 70 80 90 100
LCIECQANTD SAAAQECTVA WGTCNHAFHF HCISRWLNTR NVCPLDNREW

EFQRYGH
Length:107
Mass (Da):12,724
Last modified:May 1, 2000 - v2
Checksum:i71C3A3D2BF26DABF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF179228 mRNA Translation: AAD54393.1
CU329670 Genomic DNA Translation: CAB58559.1
PIRiT38310
RefSeqiNP_593388.1, NM_001018820.2

Genome annotation databases

EnsemblFungiiSPAC23H4.18c.1; SPAC23H4.18c.1:pep; SPAC23H4.18c
GeneIDi2541897
KEGGispo:SPAC23H4.18c

Similar proteinsi

Entry informationi

Entry nameiRBX1_SCHPO
AccessioniPrimary (citable) accession number: O13959
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: May 1, 2000
Last modified: June 20, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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