pip1

Functionⁱ

Component of E3 ubiquitin ligase SCF complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recruit the E2 ubiquitination enzyme, like UBC3/CDC34, to the complex and brings it into close proximity to the substrate. Component of the rik1-associated E3 ubiquitin ligase complex that shows ubiquitin ligase activity and is required for histone H3K9 methylation. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting swi6/HP1 to methylated histones which leads to transcriptional silencing within centromeric heterochromatin, telomeric regions and at the silent mating-type loci.1 Publication

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	41	Zinc 1By similarity	1
Metal bindingⁱ	44	Zinc 1By similarity	1
Metal bindingⁱ	52	Zinc 3By similarity	1
Metal bindingⁱ	55	Zinc 3By similarity	1
Metal bindingⁱ	67	Zinc 3By similarity	1
Metal bindingⁱ	74	Zinc 2By similarity	1
Metal bindingⁱ	76	Zinc 2By similarity	1
Metal bindingⁱ	79	Zinc 1By similarity	1
Metal bindingⁱ	81	Zinc 3By similarity	1
Metal bindingⁱ	82	Zinc 1By similarity	1
Metal bindingⁱ	93	Zinc 2By similarity	1
Metal bindingⁱ	96	Zinc 2By similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	52 – 97	RING-typePROSITE-ProRule annotationAdd BLAST		46

GO - Molecular functionⁱ

cullin family protein binding Source: GO_Central
NEDD8 transferase activity Source: GO_Central
zinc ion binding Source: PomBase

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

chromatin organization Source: UniProtKB-KW
protein modification by small protein conjugation or removal Source: PomBase
protein neddylation Source: GO_Central
protein ubiquitination Source: UniProtKB-UniPathway
regulation of transcription, DNA-templated Source: UniProtKB-KW
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
Source: PomBase
Inferred from direct assayⁱ
- 25165823
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chromatin regulator
Biological process	Transcription, Transcription regulation, Ubl conjugation pathway
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-SPO-110314 Recognition of DNA damage by PCNA-containing replication complex R-SPO-5632684 Hedgehog 'on' state R-SPO-5696395 Formation of Incision Complex in GG-NER R-SPO-5696400 Dual Incision in GG-NER R-SPO-6781823 Formation of TC-NER Pre-Incision Complex R-SPO-6782135 Dual incision in TC-NER R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-SPO-68949 Orc1 removal from chromatin R-SPO-8951664 Neddylation R-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayⁱ	UPA00143

Reactomeⁱ

R-SPO-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SPO-5632684 Hedgehog 'on' state
R-SPO-5696395 Formation of Incision Complex in GG-NER
R-SPO-5696400 Dual Incision in GG-NER
R-SPO-6781823 Formation of TC-NER Pre-Incision Complex
R-SPO-6782135 Dual incision in TC-NER
R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SPO-68949 Orc1 removal from chromatin
R-SPO-8951664 Neddylation
R-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation

UniPathwayⁱ

UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: RING-box protein pip1 Short name: RING-box protein 1 Alternative name(s): Pop-interacting protein 1
Gene namesⁱ	Name:pip1 ORF Names:SPAC23H4.18c
Organismⁱ	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifierⁱ	284812 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe
Proteomesⁱ	UP000002485 Componentⁱ: Chromosome I

Organism-specific databases

EuPathDBⁱ	FungiDB:SPAC23H4.18c
PomBaseⁱ	SPAC23H4.18c

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000056021	1 – 107	RING-box protein pip1Add BLAST		107

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O13959
PaxDbⁱ	O13959
PRIDEⁱ	O13959

Interactionⁱ

Subunit structureⁱ

Part of a SCF E3 ubiquitin ligase complex containing psh1, pip1, pcul1 and the F-box proteins pop1 and pop2. Instead of the pop1/pop2 heterodimer also homooligomers of pop1 or pop2 may be present in the complex. Component of the rik1-associated E3 ubiquitin ligase complex composed of at least clr4, cul4, pip1, raf1 and raf2. Interacts with cul3.3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
skp1	Q9Y709	6	EBI-1112060,EBI-1172248

With

Entry

#Exp.

IntAct

Notes

skp1

Q9Y709

6

EBI-1112060,EBI-1172248

GO - Molecular functionⁱ

cullin family protein binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	278387, 11 interactors
IntActⁱ	O13959, 9 interactors
STRINGⁱ	4896.SPAC23H4.18c.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O13959
SMRⁱ	O13959
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domainⁱ

The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	52 – 97	RING-typePROSITE-ProRule annotationAdd BLAST		46

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

HOGENOMⁱ	HOG000171951
InParanoidⁱ	O13959
KEGG Orthology (KO) More...KOⁱ	K03868
OMAⁱ	NHIMEPC
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C4PUH
PhylomeDBⁱ	O13959

Family and domain databases

Gene3Dⁱ	3.30.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD IPR024766 Znf_RING_H2
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12678 zf-rbx1, 1 hit
PROSITEⁱ	View protein in PROSITE PS50089 ZF_RING_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

O13959-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEDEMQIDKK EVEIEQKPPR FEIKKWNAVA LWQWDIVVDN CAICRNHIMD 
        60         70         80         90        100
LCIECQANTD SAAAQECTVA WGTCNHAFHF HCISRWLNTR NVCPLDNREW 

EFQRYGH

Length:107

Mass (Da):12,724

Last modified:May 1, 2000 - v2

Checksum:ⁱ71C3A3D2BF26DABF

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF179228 mRNA Translation: AAD54393.1 CU329670 Genomic DNA Translation: CAB58559.1
PIRⁱ	T38310
NCBI Reference Sequences More...RefSeqⁱ	NP_593388.1, NM_001018820.2

Genome annotation databases

EnsemblFungiⁱ	SPAC23H4.18c.1; SPAC23H4.18c.1:pep; SPAC23H4.18c
GeneIDⁱ	2541897
KEGGⁱ	spo:SPAC23H4.18c

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length
O13959	E3 ubiquitin-protein ligase RBX1		PLAGO		108
UPI000A2A8A41		EXAPA		112
Ubiquitin ligase subunit		Hypoxylon sp. EC38		122
Uncharacterized protein		Hypoxylon sp. CO27-5		122
Uncharacterized protein		Hypoxylon sp. CI-4A		123
+64

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF179228 mRNA Translation: AAD54393.1 CU329670 Genomic DNA Translation: CAB58559.1
PIRⁱ	T38310
NCBI Reference Sequences More...RefSeqⁱ	NP_593388.1, NM_001018820.2

3D structure databases

ProteinModelPortalⁱ	O13959
SMRⁱ	O13959
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	278387, 11 interactors
IntActⁱ	O13959, 9 interactors
STRINGⁱ	4896.SPAC23H4.18c.1

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O13959
PaxDbⁱ	O13959
PRIDEⁱ	O13959

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	SPAC23H4.18c.1; SPAC23H4.18c.1:pep; SPAC23H4.18c
GeneIDⁱ	2541897
KEGGⁱ	spo:SPAC23H4.18c

Organism-specific databases

EuPathDBⁱ	FungiDB:SPAC23H4.18c
PomBaseⁱ	SPAC23H4.18c

Phylogenomic databases

HOGENOMⁱ	HOG000171951
InParanoidⁱ	O13959
KEGG Orthology (KO) More...KOⁱ	K03868
OMAⁱ	NHIMEPC
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C4PUH
PhylomeDBⁱ	O13959

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
Reactomeⁱ	R-SPO-110314 Recognition of DNA damage by PCNA-containing replication complex R-SPO-5632684 Hedgehog 'on' state R-SPO-5696395 Formation of Incision Complex in GG-NER R-SPO-5696400 Dual Incision in GG-NER R-SPO-6781823 Formation of TC-NER Pre-Incision Complex R-SPO-6782135 Dual incision in TC-NER R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-SPO-68949 Orc1 removal from chromatin R-SPO-8951664 Neddylation R-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation

UniPathwayⁱ

UPA00143

Reactomeⁱ

R-SPO-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SPO-5632684 Hedgehog 'on' state
R-SPO-5696395 Formation of Incision Complex in GG-NER
R-SPO-5696400 Dual Incision in GG-NER
R-SPO-6781823 Formation of TC-NER Pre-Incision Complex
R-SPO-6782135 Dual incision in TC-NER
R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SPO-68949 Orc1 removal from chromatin
R-SPO-8951664 Neddylation
R-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O13959

PROⁱ

PR:O13959

Family and domain databases

Gene3Dⁱ	3.30.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD IPR024766 Znf_RING_H2
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12678 zf-rbx1, 1 hit
PROSITEⁱ	View protein in PROSITE PS50089 ZF_RING_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RBX1_SCHPO
Accessionⁱ	O13959Primary (citable) accession number: O13959
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
	Last sequence update:	May 1, 2000
	Last modified:	June 20, 2018
	This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O13959 (RBX1_SCHPO)

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RING-box protein pip1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Zinc finger

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ