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Protein

Serine/threonine-protein kinase srb10

Gene

srb10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the Cdk8 module/Srb8-11 module which is a regulatory module of the Mediator complex that regulates basal RNA polymerase II transcription. The Cdk8 module may sterically hinder the interaction between Mediator and RNA polymerase II leading to transcriptional repression of a subset of genes regulated by Mediator.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36ATPPROSITE-ProRule annotation1
Active sitei140Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 19ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter Source: PomBase
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • regulation of transcription from RNA polymerase II promoter Source: PomBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 5613.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase srb10 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cyclin-dependent kinase 8
Suppressor of RNA polymerase B srb10
Gene namesi
Name:srb10
Synonyms:cdk8, prk1
ORF Names:SPAC23H4.17c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23H4.17c.
PomBaseiSPAC23H4.17c. srb10.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi158D → A: Abrogates kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865801 – 369Serine/threonine-protein kinase srb10Add BLAST369

Proteomic databases

MaxQBiO13958.
PRIDEiO13958.

Interactioni

Subunit structurei

Component of the Cdk8 module of the Mediator complex. The Cdk8 module is composed of srb8, srb9, srb10 and srb11. Interacts with med17 and med18.2 Publications

Protein-protein interaction databases

BioGridi278390. 249 interactors.

Structurei

3D structure databases

ProteinModelPortaliO13958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST315

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233024.
InParanoidiO13958.
KOiK02208.
OMAiREIQHEN.
OrthoDBiEOG092C2FL8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDGYKIIGF ISSGTYGKVY KAVSSNSNDK RLFAIKKFKA ESKQVSSNAQ
60 70 80 90 100
QTGVSQSAIR EMMLCREIQH ENIVSLVQVL LKDGTISMVF EYAEHDLLQI
110 120 130 140 150
IHFHSRSRTR QIPPSILKSI LWQIINGVAY LHENWIMHRD LKPANIMITA
160 170 180 190 200
TGKVKIGDLG LGRLIRDPIL PFYSSDRVVV TIWYRAPELL LGAHDYTPAI
210 220 230 240 250
DVWAIGCIYG EMLALSPLFK GDEIKMEDKK VVPFQSTQML RIMELLGTPT
260 270 280 290 300
EERWPGLKNY PEYYQLSSFE VRYWNNLLPQ WYQTVKNRDP QGLDLLMKML
310 320 330 340 350
QYDPKSRITA KQALEHVFFT SDKLWTTSPF LNQPIHYPER RISEDDSEVS
360
SKRVLSTSLR SESKRFKGN
Length:369
Mass (Da):42,585
Last modified:April 18, 2012 - v2
Checksum:iC7F9AC9F73FFD30E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11671.2.
PIRiT38311.
RefSeqiNP_593389.2. NM_001018821.2.

Genome annotation databases

EnsemblFungiiSPAC23H4.17c.1; SPAC23H4.17c.1:pep; SPAC23H4.17c.
GeneIDi2541900.
KEGGispo:SPAC23H4.17c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11671.2.
PIRiT38311.
RefSeqiNP_593389.2. NM_001018821.2.

3D structure databases

ProteinModelPortaliO13958.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278390. 249 interactors.

Proteomic databases

MaxQBiO13958.
PRIDEiO13958.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC23H4.17c.1; SPAC23H4.17c.1:pep; SPAC23H4.17c.
GeneIDi2541900.
KEGGispo:SPAC23H4.17c.

Organism-specific databases

EuPathDBiFungiDB:SPAC23H4.17c.
PomBaseiSPAC23H4.17c. srb10.

Phylogenomic databases

HOGENOMiHOG000233024.
InParanoidiO13958.
KOiK02208.
OMAiREIQHEN.
OrthoDBiEOG092C2FL8.

Enzyme and pathway databases

BRENDAi2.7.11.22. 5613.

Miscellaneous databases

PROiO13958.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSN3_SCHPO
AccessioniPrimary (citable) accession number: O13958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: April 18, 2012
Last modified: October 5, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.