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Protein

Serine/threonine-protein kinase srb10

Gene

srb10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic component of the Cdk8 module/Srb8-11 module which is a regulatory module of the Mediator complex that regulates basal RNA polymerase II transcription. The Cdk8 module may sterically hinder the interaction between Mediator and RNA polymerase II leading to transcriptional repression of a subset of genes regulated by Mediator.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36ATPPROSITE-ProRule annotation1
Active sitei140Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 19ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter Source: PomBase
  • phosphorylation of RNA polymerase II C-terminal domain Source: PomBase
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • regulation of transcription by RNA polymerase II Source: PomBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase
Biological processTranscription, Transcription regulation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22 5613

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase srb10 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cyclin-dependent kinase 8
Suppressor of RNA polymerase B srb10
Gene namesi
Name:srb10
Synonyms:cdk8, prk1
ORF Names:SPAC23H4.17c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23H4.17c
PomBaseiSPAC23H4.17c srb10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi158D → A: Abrogates kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865801 – 369Serine/threonine-protein kinase srb10Add BLAST369

Proteomic databases

MaxQBiO13958
PaxDbiO13958
PRIDEiO13958

PTM databases

iPTMnetiO13958

Interactioni

Subunit structurei

Component of the Cdk8 module of the Mediator complex. The Cdk8 module is composed of srb8, srb9, srb10 and srb11. Interacts with med17 and med18.2 Publications

Protein-protein interaction databases

BioGridi278390, 250 interactors
STRINGi4896.SPAC23H4.17c.1

Structurei

3D structure databases

ProteinModelPortaliO13958
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST315

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000233024
InParanoidiO13958
KOiK02208
OMAiKSIYMVF
OrthoDBiEOG092C2FL8

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O13958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDGYKIIGF ISSGTYGKVY KAVSSNSNDK RLFAIKKFKA ESKQVSSNAQ
60 70 80 90 100
QTGVSQSAIR EMMLCREIQH ENIVSLVQVL LKDGTISMVF EYAEHDLLQI
110 120 130 140 150
IHFHSRSRTR QIPPSILKSI LWQIINGVAY LHENWIMHRD LKPANIMITA
160 170 180 190 200
TGKVKIGDLG LGRLIRDPIL PFYSSDRVVV TIWYRAPELL LGAHDYTPAI
210 220 230 240 250
DVWAIGCIYG EMLALSPLFK GDEIKMEDKK VVPFQSTQML RIMELLGTPT
260 270 280 290 300
EERWPGLKNY PEYYQLSSFE VRYWNNLLPQ WYQTVKNRDP QGLDLLMKML
310 320 330 340 350
QYDPKSRITA KQALEHVFFT SDKLWTTSPF LNQPIHYPER RISEDDSEVS
360
SKRVLSTSLR SESKRFKGN
Length:369
Mass (Da):42,585
Last modified:April 18, 2012 - v2
Checksum:iC7F9AC9F73FFD30E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB11671.2
PIRiT38311
RefSeqiNP_593389.2, NM_001018821.2

Genome annotation databases

EnsemblFungiiSPAC23H4.17c.1; SPAC23H4.17c.1:pep; SPAC23H4.17c
GeneIDi2541900
KEGGispo:SPAC23H4.17c

Similar proteinsi

Entry informationi

Entry nameiSSN3_SCHPO
AccessioniPrimary (citable) accession number: O13958
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: April 18, 2012
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health