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O13958

- SSN3_SCHPO

UniProt

O13958 - SSN3_SCHPO

Protein

Serine/threonine-protein kinase srb10

Gene

srb10

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (18 Apr 2012)
      Previous versions | rss
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    Functioni

    Catalytic component of the Cdk8 module/Srb8-11 module which is a regulatory module of the Mediator complex that regulates basal RNA polymerase II transcription. The Cdk8 module may sterically hinder the interaction between Mediator and RNA polymerase II leading to transcriptional repression of a subset of genes regulated by Mediator.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361ATPPROSITE-ProRule annotation
    Active sitei140 – 1401Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 199ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: PomBase
    3. metal ion binding Source: UniProtKB-KW
    4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. activation of bipolar cell growth Source: PomBase
    2. negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter Source: PomBase
    3. phosphorylation of RNA polymerase II C-terminal domain Source: PomBase
    4. positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 5615.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase srb10 (EC:2.7.11.22, EC:2.7.11.23)
    Alternative name(s):
    Cyclin-dependent kinase 8
    Suppressor of RNA polymerase B srb10
    Gene namesi
    Name:srb10
    Synonyms:cdk8, prk1
    ORF Names:SPAC23H4.17c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC23H4.17c.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi158 – 1581D → A: Abrogates kinase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Serine/threonine-protein kinase srb10PRO_0000086580Add
    BLAST

    Proteomic databases

    MaxQBiO13958.

    Interactioni

    Subunit structurei

    Component of the Cdk8 module of the Mediator complex. The Cdk8 module is composed of srb8, srb9, srb10 and srb11. Interacts with med17 and med18.2 Publications

    Protein-protein interaction databases

    BioGridi278390. 247 interactions.
    STRINGi4896.SPAC23H4.17c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO13958.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 319315Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    KOiK02208.
    OrthoDBiEOG7K3TWD.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O13958-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDGYKIIGF ISSGTYGKVY KAVSSNSNDK RLFAIKKFKA ESKQVSSNAQ    50
    QTGVSQSAIR EMMLCREIQH ENIVSLVQVL LKDGTISMVF EYAEHDLLQI 100
    IHFHSRSRTR QIPPSILKSI LWQIINGVAY LHENWIMHRD LKPANIMITA 150
    TGKVKIGDLG LGRLIRDPIL PFYSSDRVVV TIWYRAPELL LGAHDYTPAI 200
    DVWAIGCIYG EMLALSPLFK GDEIKMEDKK VVPFQSTQML RIMELLGTPT 250
    EERWPGLKNY PEYYQLSSFE VRYWNNLLPQ WYQTVKNRDP QGLDLLMKML 300
    QYDPKSRITA KQALEHVFFT SDKLWTTSPF LNQPIHYPER RISEDDSEVS 350
    SKRVLSTSLR SESKRFKGN 369
    Length:369
    Mass (Da):42,585
    Last modified:April 18, 2012 - v2
    Checksum:iC7F9AC9F73FFD30E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11671.2.
    PIRiT38311.
    RefSeqiNP_593389.2. NM_001018821.2.

    Genome annotation databases

    EnsemblFungiiSPAC23H4.17c.1; SPAC23H4.17c.1:pep; SPAC23H4.17c.
    GeneIDi2541900.
    KEGGispo:SPAC23H4.17c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11671.2 .
    PIRi T38311.
    RefSeqi NP_593389.2. NM_001018821.2.

    3D structure databases

    ProteinModelPortali O13958.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 278390. 247 interactions.
    STRINGi 4896.SPAC23H4.17c-1.

    Proteomic databases

    MaxQBi O13958.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC23H4.17c.1 ; SPAC23H4.17c.1:pep ; SPAC23H4.17c .
    GeneIDi 2541900.
    KEGGi spo:SPAC23H4.17c.

    Organism-specific databases

    PomBasei SPAC23H4.17c.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    KOi K02208.
    OrthoDBi EOG7K3TWD.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 5615.

    Miscellaneous databases

    NextBioi 20802987.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the prk1 protein kinase from Schizosaccharomyces pombe."
      Watson P., Davey J.
      Yeast 14:485-492(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Comparative functional genomics of the fission yeasts."
      Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
      , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
      Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVISION OF GENE MODEL.
    4. "TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution."
      Samuelsen C.O., Baraznenok V., Khorosjutina O., Spaehr H., Kieselbach T., Holmberg S., Gustafsson C.M.
      Proc. Natl. Acad. Sci. U.S.A. 100:6422-6427(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN MEDIATOR COMPLEX.
    5. "Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro."
      Spaehr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen C.O., Hermand D., Maekelae T.P., Holmberg S., Gustafsson C.M.
      J. Biol. Chem. 278:51301-51306(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II."
      Elmlund H., Baraznenok V., Lindahl M., Samuelsen C.O., Koeck P.J.B., Holmberg S., Hebert H., Gustafsson C.M.
      Proc. Natl. Acad. Sci. U.S.A. 103:15788-15793(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF THE MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II, INTERACTION WITH MED17 AND MED18, MUTAGENESIS OF ASP-158.

    Entry informationi

    Entry nameiSSN3_SCHPO
    AccessioniPrimary (citable) accession number: O13958
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: April 18, 2012
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3