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O13958

- SSN3_SCHPO

UniProt

O13958 - SSN3_SCHPO

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Protein
Serine/threonine-protein kinase srb10
Gene
srb10, cdk8, prk1, SPAC23H4.17c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic component of the Cdk8 module/Srb8-11 module which is a regulatory module of the Mediator complex that regulates basal RNA polymerase II transcription. The Cdk8 module may sterically hinder the interaction between Mediator and RNA polymerase II leading to transcriptional repression of a subset of genes regulated by Mediator.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361ATP By similarity
Active sitei140 – 1401Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 199ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC
  3. cyclin-dependent protein serine/threonine kinase activity Source: PomBase
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. activation of bipolar cell growth Source: PomBase
  2. negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter Source: PomBase
  3. phosphorylation of RNA polymerase II C-terminal domain Source: PomBase
  4. positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 5615.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase srb10 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cyclin-dependent kinase 8
Suppressor of RNA polymerase B srb10
Gene namesi
Name:srb10
Synonyms:cdk8, prk1
ORF Names:SPAC23H4.17c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC23H4.17c.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. mediator complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi158 – 1581D → A: Abrogates kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Serine/threonine-protein kinase srb10
PRO_0000086580Add
BLAST

Proteomic databases

MaxQBiO13958.

Interactioni

Subunit structurei

Component of the Cdk8 module of the Mediator complex. The Cdk8 module is composed of srb8, srb9, srb10 and srb11. Interacts with med17 and med18.2 Publications

Protein-protein interaction databases

BioGridi278390. 247 interactions.
STRINGi4896.SPAC23H4.17c-1.

Structurei

3D structure databases

ProteinModelPortaliO13958.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 319315Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
KOiK02208.
OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13958-1 [UniParc]FASTAAdd to Basket

« Hide

MKDGYKIIGF ISSGTYGKVY KAVSSNSNDK RLFAIKKFKA ESKQVSSNAQ    50
QTGVSQSAIR EMMLCREIQH ENIVSLVQVL LKDGTISMVF EYAEHDLLQI 100
IHFHSRSRTR QIPPSILKSI LWQIINGVAY LHENWIMHRD LKPANIMITA 150
TGKVKIGDLG LGRLIRDPIL PFYSSDRVVV TIWYRAPELL LGAHDYTPAI 200
DVWAIGCIYG EMLALSPLFK GDEIKMEDKK VVPFQSTQML RIMELLGTPT 250
EERWPGLKNY PEYYQLSSFE VRYWNNLLPQ WYQTVKNRDP QGLDLLMKML 300
QYDPKSRITA KQALEHVFFT SDKLWTTSPF LNQPIHYPER RISEDDSEVS 350
SKRVLSTSLR SESKRFKGN 369
Length:369
Mass (Da):42,585
Last modified:April 18, 2012 - v2
Checksum:iC7F9AC9F73FFD30E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAB11671.2.
PIRiT38311.
RefSeqiNP_593389.2. NM_001018821.2.

Genome annotation databases

EnsemblFungiiSPAC23H4.17c.1; SPAC23H4.17c.1:pep; SPAC23H4.17c.
GeneIDi2541900.
KEGGispo:SPAC23H4.17c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAB11671.2 .
PIRi T38311.
RefSeqi NP_593389.2. NM_001018821.2.

3D structure databases

ProteinModelPortali O13958.
ModBasei Search...

Protein-protein interaction databases

BioGridi 278390. 247 interactions.
STRINGi 4896.SPAC23H4.17c-1.

Proteomic databases

MaxQBi O13958.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC23H4.17c.1 ; SPAC23H4.17c.1:pep ; SPAC23H4.17c .
GeneIDi 2541900.
KEGGi spo:SPAC23H4.17c.

Organism-specific databases

PomBasei SPAC23H4.17c.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
KOi K02208.
OrthoDBi EOG7K3TWD.

Enzyme and pathway databases

BRENDAi 2.7.11.22. 5615.

Miscellaneous databases

NextBioi 20802987.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the prk1 protein kinase from Schizosaccharomyces pombe."
    Watson P., Davey J.
    Yeast 14:485-492(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Comparative functional genomics of the fission yeasts."
    Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
    , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
    Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  4. "TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution."
    Samuelsen C.O., Baraznenok V., Khorosjutina O., Spaehr H., Kieselbach T., Holmberg S., Gustafsson C.M.
    Proc. Natl. Acad. Sci. U.S.A. 100:6422-6427(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN MEDIATOR COMPLEX.
  5. "Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro."
    Spaehr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen C.O., Hermand D., Maekelae T.P., Holmberg S., Gustafsson C.M.
    J. Biol. Chem. 278:51301-51306(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II."
    Elmlund H., Baraznenok V., Lindahl M., Samuelsen C.O., Koeck P.J.B., Holmberg S., Hebert H., Gustafsson C.M.
    Proc. Natl. Acad. Sci. U.S.A. 103:15788-15793(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY OF THE MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II, INTERACTION WITH MED17 AND MED18, MUTAGENESIS OF ASP-158.

Entry informationi

Entry nameiSSN3_SCHPO
AccessioniPrimary (citable) accession number: O13958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: April 18, 2012
Last modified: May 14, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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