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Protein

Mitochondrial tRNA-specific 2-thiouridylase 1

Gene

SPAC23H4.04

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.By similarity

Catalytic activityi

A [protein]-S-sulfanyl-L-cysteine + 5-taurinomethyluridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 5-taurinomethyl-2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei129NucleophileBy similarity1
Binding sitei159ATP; via amide nitrogenBy similarity1
Active sitei234Cysteine persulfide intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 44ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial tRNA-specific 2-thiouridylase 1 (EC:2.8.1.14By similarity)
Gene namesi
ORF Names:SPAC23H4.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23H4.04.
PomBaseiSPAC23H4.04.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217101 – 415Mitochondrial tRNA-specific 2-thiouridylase 1Add BLAST415

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi129 ↔ 234AlternateBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiO13947.
PRIDEiO13947.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei160Interaction with tRNABy similarity1
Sitei285Interaction with tRNABy similarity1
Sitei389Interaction with tRNABy similarity1

Structurei

3D structure databases

ProteinModelPortaliO13947.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 126Interaction with target base in tRNABy similarity3
Regioni183 – 185Interaction with tRNABy similarity3
Regioni356 – 357Interaction with tRNABy similarity2

Sequence similaritiesi

Belongs to the MnmA/TRMU family.Curated

Phylogenomic databases

HOGENOMiHOG000218046.
InParanoidiO13947.
KOiK00566.
OMAiFACTAKF.
OrthoDBiEOG092C27IL.
PhylomeDBiO13947.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

O13947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVSLFLQKQ IIECSKAFQP HSTRLQWPKS QDKVFVAMSG GVDSSFSAYL
60 70 80 90 100
LKSQGYNVEG VFMRNWLDED SAPSGCPAER DWATVQKVCK KLNISCRRFN
110 120 130 140 150
FEKEYWNLVF EPSLDLYENG LTPNPDVSCN RQVKFGALFD ALKKHCENNV
160 170 180 190 200
KGDWWLASGH YAKSVVNIET NESHMCIPTD KRKDQTLFLC TIRKEALEKT
210 220 230 240 250
IFPLHNWTKE NVKKQASSAG FKEIAEKQES QGLCFVSPNV GRKFRKFLQR
260 270 280 290 300
YLNFSDRPIK VIAGKNVVGE FSGNHGIWSL TVGERCGLSL PQAQSEYFGR
310 320 330 340 350
WYVWKKDIKN NALYICRGTN NELLMSKCIY LKDWKWCGTK LQNLEKSALS
360 370 380 390 400
CFVRVRHQQP LQPAKVTWRN PESVKIHFQD KQRAVTPGQV IAVYVNDVCL
410
GGGMVDTVEP EKDFD
Length:415
Mass (Da):47,627
Last modified:January 1, 1998 - v1
Checksum:iD2B604335B7A935F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11659.1.
PIRiT38324.
RefSeqiNP_593402.1. NM_001018834.2.

Genome annotation databases

EnsemblFungiiSPAC23H4.04.1; SPAC23H4.04.1:pep; SPAC23H4.04.
GeneIDi2541868.
KEGGispo:SPAC23H4.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11659.1.
PIRiT38324.
RefSeqiNP_593402.1. NM_001018834.2.

3D structure databases

ProteinModelPortaliO13947.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

MaxQBiO13947.
PRIDEiO13947.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC23H4.04.1; SPAC23H4.04.1:pep; SPAC23H4.04.
GeneIDi2541868.
KEGGispo:SPAC23H4.04.

Organism-specific databases

EuPathDBiFungiDB:SPAC23H4.04.
PomBaseiSPAC23H4.04.

Phylogenomic databases

HOGENOMiHOG000218046.
InParanoidiO13947.
KOiK00566.
OMAiFACTAKF.
OrthoDBiEOG092C27IL.
PhylomeDBiO13947.

Miscellaneous databases

PROiO13947.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTU1_SCHPO
AccessioniPrimary (citable) accession number: O13947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

During the reaction, ATP is used to activate the C2 atom of U34 by adenylation. After this, the persulfide sulfur on the catalytic cysteine is transferred to the C2 atom of the wobble base (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards the activated C2 atom on U34. Subsequently, a transient disulfide bond is formed between the two active site cysteine residues (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.