Reviewed, UniProtKB/Swiss-Prot O13935 (YF6H_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative tRNA (cytosine-5-)-methyltransferase C23C4.17
    EC=2.1.1.29

Gene names

ORF Names:

SPAC23C4.17

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Methylates cytosine to m5C at several positions in different tRNAs and pre-tRNAs containing intron By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.
Subcellular location	Nucleus. Ref.2
Sequence similarities	Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	tRNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	Putative tRNA (cytosine-5-)-methyltransferase C23C4.17		PRO_0000317151
Regions
	Region	167 – 173	7	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	323	1	Nucleophile Potential
	Binding site	208	1	S-adenosyl-L-methionine By similarity
	Binding site	235	1	S-adenosyl-L-methionine By similarity
	Binding site	270	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O13935-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 10C4EA8B147363D3	FASTA	674	76,602
	10 20 30 40 50 60 MGKRNKKVTQ GKRAYNDKSE IVLENKQFEG YYKKQNLFRG KPNDEFDSFM EYMRKPLPTT 70 80 90 100 110 120 FRICGYRHHA FELKNHFEKY YVPSLKNVVH EGQTIPPPTV LPWYPDGLAY IVDAQKDVIR 130 140 150 160 170 180 KSPPLKRLQR FLVSENEAGN INRQEAVSML PPLFLDVEPH HVILDMCAAP GSKTAQLIEA 190 200 210 220 230 240 VYKKANIKDA AHDSKNLKSV EGLVIANDAD PKRAQMLVHQ INRLNSPNIL VVNHDASTMP 250 260 270 280 290 300 NIYVKGSSPS DGLNVIEEKK ILKFDRILAD VPCSGDGTFR KNLSLWREWS ANSAFSLHPL 310 320 330 340 350 360 QLRILIRGLQ LLKVGGCLVY STCSINPIEN EAVVTAALKA TGGAVSLVDV SKKLPLLKRD 370 380 390 400 410 420 PGLLSWKVLD DSLNEFQSPA ENTNDKIELT ESMWPLPEEE MSKLHIERCA RLYPHMQNTG 430 440 450 460 470 480 GFFVAVLQKT DPINSRSFDP KKYTASMEIL PPENKRQRTE KGVDEASNST LTKSGNSYFD 490 500 510 520 530 540 EEPFVYINPD DTSIKTIVDF YGIDPSFPRD QFFVRNQSGI PVRSIYFACS LFKEIIEANT 550 560 570 580 590 600 NRVKFVHGGV RFFVKQEISQ LLKDFSLKAN KDICNFRIHS NGVNIISPFL NEKHFYDAGL 610 620 630 640 650 660 KDLKILVKNE YPHVEQFSES GMLKKEFEKM PLGCNILRVD AQTKDGALMD MLILQPIWRS 670 PTSCNLMLAR KEKQ

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB16888.1.
PIR	T38272.
RefSeq	NP_593189.1.
3D structure databases
HSSP	HSSP built from PDB template 1IXK based on UniProtKB O50082.
ModBase	Search...
Genome annotation databases
GeneID	2541917.
KEGG	spo:SPAC23C4.17.
NMPDR	fig|4896.1.peg.3159.
Organism-specific databases
GeneDB_Spombe	SPAC23C4.17.
Phylogenomic databases
OMA	O13935. IANDINP.
Enzyme and pathway databases
BRENDA	2.1.1.29. 653.
Gene expression databases
ArrayExpress	O13935.
Family and domain databases
InterPro	IPR001678. Fmu/NOL1/Nop2p. IPR018314. Fmu/NOL1/Nop2p_CS. [Graphical view]
Pfam	PF01189. Nol1_Nop2_Fmu. 2 hits. [Graphical view]
PROSITE	PS01153. NOL1_NOP2_SUN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YF6H_SCHPO

Accession

Primary (citable) accession number: O13935

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)