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O13935 (YF6H_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative tRNA (cytosine-5-)-methyltransferase C23C4.17
EC=2.1.1.-
Gene names
ORF Names:SPAC23C4.17
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length674 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Methylates cytosine to m5C at several positions in different tRNAs and pre-tRNAs containing intron By similarity.

Catalytic activity

S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.

Subcellular location

Nucleus Ref.2.

Sequence similarities

Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentnucleus

Inferred from direct assay. Source: GeneDB_Spombe

   Molecular functiontRNA (cytosine-5-)-methyltransferase activity

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 674674Putative tRNA (cytosine-5-)-methyltransferase C23C4.17
PRO_0000317151

Regions

Region167 – 1737S-adenosyl-L-methionine binding By similarity

Sites

Active site3231Nucleophile Potential
Binding site2081S-adenosyl-L-methionine By similarity
Binding site2351S-adenosyl-L-methionine By similarity
Binding site2701S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
O13935 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 10C4EA8B147363D3

FASTA67476,602
        10         20         30         40         50         60 
MGKRNKKVTQ GKRAYNDKSE IVLENKQFEG YYKKQNLFRG KPNDEFDSFM EYMRKPLPTT 

        70         80         90        100        110        120 
FRICGYRHHA FELKNHFEKY YVPSLKNVVH EGQTIPPPTV LPWYPDGLAY IVDAQKDVIR 

       130        140        150        160        170        180 
KSPPLKRLQR FLVSENEAGN INRQEAVSML PPLFLDVEPH HVILDMCAAP GSKTAQLIEA 

       190        200        210        220        230        240 
VYKKANIKDA AHDSKNLKSV EGLVIANDAD PKRAQMLVHQ INRLNSPNIL VVNHDASTMP 

       250        260        270        280        290        300 
NIYVKGSSPS DGLNVIEEKK ILKFDRILAD VPCSGDGTFR KNLSLWREWS ANSAFSLHPL 

       310        320        330        340        350        360 
QLRILIRGLQ LLKVGGCLVY STCSINPIEN EAVVTAALKA TGGAVSLVDV SKKLPLLKRD 

       370        380        390        400        410        420 
PGLLSWKVLD DSLNEFQSPA ENTNDKIELT ESMWPLPEEE MSKLHIERCA RLYPHMQNTG 

       430        440        450        460        470        480 
GFFVAVLQKT DPINSRSFDP KKYTASMEIL PPENKRQRTE KGVDEASNST LTKSGNSYFD 

       490        500        510        520        530        540 
EEPFVYINPD DTSIKTIVDF YGIDPSFPRD QFFVRNQSGI PVRSIYFACS LFKEIIEANT 

       550        560        570        580        590        600 
NRVKFVHGGV RFFVKQEISQ LLKDFSLKAN KDICNFRIHS NGVNIISPFL NEKHFYDAGL 

       610        620        630        640        650        660 
KDLKILVKNE YPHVEQFSES GMLKKEFEKM PLGCNILRVD AQTKDGALMD MLILQPIWRS 

       670 
PTSCNLMLAR KEKQ

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329670 Genomic DNA. Translation: CAB16888.1.
PIRT38272.
RefSeqNP_593189.1. NM_001018585.1.

3D structure databases

HSSPHSSP built from PDB template 2B9E based on UniProtKB Q96P11.
ProteinModelPortalO13935.
ModBaseSearch...

Protein-protein interaction databases

STRINGO13935.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2541917.
KEGGspo:SPAC23C4.17.
NMPDRfig|4896.1.peg.3159.

Organism-specific databases

GeneDB_SpombeSPAC23C4.17.

Phylogenomic databases

eggNOGfuNOG04985.
GeneTreeEFGT00050000002506.
HOGENOMHBG315458.
OMAHYIPEIT.
OrthoDBEOG4RFQ1S.

Gene expression databases

ArrayExpressO13935.

Family and domain databases

InterProIPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR023267. RCMT.
IPR023270. RCMT_NCL1.
[Graphical view]
KOK15334.
PfamPF01189. Nol1_Nop2_Fmu. 1 hit.
[Graphical view]
PRINTSPR02008. RCMTFAMILY.
PR02011. RCMTNCL1.
PROSITEPS01153. NOL1_NOP2_SUN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameYF6H_SCHPO
AccessionPrimary (citable) accession number: O13935
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 1, 1998
Last modified: December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents