O13934 (ATG15_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipase atg15
EC=3.1.1.3

Alternative name(s):
Autophagy-related protein 15

Gene names

Name:	atg15
ORF Names:	SPAC23C4.16c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ascospore formation Inferred from mutant phenotype. Source: GeneDB_Spombe cellular response to nitrogen starvation Inferred from mutant phenotype. Source: GeneDB_Spombe lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW macroautophagy Inferred by curator. Source: GeneDB_Spombe membrane disassembly Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW multivesicular body membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuolar lumen Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Putative lipase atg15		PRO_0000090370
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 424	394	Lumenal Potential
Sites
	Active site	283	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	187	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O13934 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8B8BA13645C5097E	FASTA	424	47,938
	10 20 30 40 50 60 MSQLLFLRRF FFLFCFIIRI SCTGVFESVI KSSENVPDKV NVKLQHVFHY GLNEDSISYY 70 80 90 100 110 120 RLDVAKKSVY AESESKLPLK MKKGYSVHLK DQSRDALTDY RYKSMKGIYS EANSPADLWE 130 140 150 160 170 180 QEAIVIPDIT DKETIYSLGK MSYNAYQKIP FDGDWLDLGP DWNITPPEGF GWEEDGLRGH 190 200 210 220 230 240 VFSNDDNSTI IIAMKGTSIF GIGRGTSQKD RVNDNLLFSC CCARVSWAWS TVCGCYKNTY 250 260 270 280 290 300 TCSQTCLEDE VQDDSRYYSA SLDIFYSVKE LYPDAQIWLT GHSLGGATAA LMGLSFGIPT 310 320 330 340 350 360 VTFEAPGDRM AARRLHLPMP PGLPDEESLV WHFGHNADPI YLGKCTGPTS LCWAGGYAME 370 380 390 400 410 420 STCHTGQECL YDVVKDKGWH LSITHHRMQT VLNDVIDAYE KLPDCSHTPN CVDCYLWEFP DDDS

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB16887.1.
PIR	T38271.
RefSeq	NP_593188.1. NM_001018584.1.
3D structure databases
ProteinModelPortal	O13934.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC23C4.16c.1; SPAC23C4.16c.1:pep; SPAC23C4.16c.
GeneID	2541924.
GenomeReviews	Gene locus atg15 in contig CU329670_GR.
KEGG	spo:SPAC23C4.16c.
NMPDR	fig|4896.1.peg.3158.
Organism-specific databases
GeneDB_Spombe	SPAC23C4.16c.
Phylogenomic databases
eggNOG	fuNOG04745.
GeneTree	EFGT00050000002657.
HOGENOM	HBG398588.
OMA	CCCARID.
OrthoDB	EOG4F7RV2.
Gene expression databases
ArrayExpress	O13934.
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_SCHPO

Accession

Primary (citable) accession number: O13934

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program