O13933 (ALG1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase
EC=2.4.1.142

Alternative name(s):
Asparagine-linked glycosylation protein 1
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase

Gene names

Name:	alg1
ORF Names:	SPAC23C4.14

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER By similarity.
Catalytic activity	GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 33 subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	oligosaccharide-lipid intermediate biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitobiosyldiphosphodolichol beta-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Chitobiosyldiphosphodolichol beta-mannosyltransferase		PRO_0000080257
Regions
	Transmembrane	1 – 24	24	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	25 – 424	400	Lumenal Potential
Amino acid modifications
	Glycosylation	374	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O13933 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: E652399C61A28579	FASTA	424	48,726
	10 20 30 40 50 60 MLVLKIVLFL SLVIWFNLKK RTDKKRIIVL VLGDIARSPR MQYHAVSFAK LGWKVDLLGY 70 80 90 100 110 120 QHPGSSVGLF ESHENIRFYP IPSLPAYLQP KNRLQFLFLG PLKVLHQFLA LNWALFVRKP 130 140 150 160 170 180 ASFLFIQNPP CIPVFFIAQC LHILRGTKFI IDWHNFGYSI LALKLGKQHT FVKLLKIYEK 190 200 210 220 230 240 YMARGAYAHL TVSKRMKDVL QTWGMNPCYV CYDRPPNHFT PIKNEQKKQM SIKKIPCEYN 250 260 270 280 290 300 PSSTKLLITS TSWTPDEDIY ILWEALNEYD KTLDTPKLLV LITGKGPMKE EFSQYIKKHP 310 320 330 340 350 360 LHKVRFCMPW LSIEDYPQVM ACADLGVCLH TSSSGLDLPM KVVDLFGCGV PVIALSYPTI 370 380 390 400 410 420 SELVHDGENG LIVNDSKALS KKMQYLLTHA NELNSLKLGA LKESEYRWDD EWNKVIPPIV QGSN

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB16885.2.
PIR	T38269.
RefSeq	NP_593186.1. NM_001018582.1.
3D structure databases
ProteinModelPortal	O13933.
ModBase	Search...
Protein-protein interaction databases
STRING	O13933.
Protein family/group databases
CAZy	GT33. Glycosyltransferase Family 33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC23C4.14.1; SPAC23C4.14.1:pep; SPAC23C4.14.
GeneID	2541937.
GenomeReviews	Gene locus alg1 in contig CU329670_GR.
KEGG	spo:SPAC23C4.14.
NMPDR	fig|4896.1.peg.3156.
Organism-specific databases
GeneDB_Spombe	SPAC23C4.14.
Phylogenomic databases
eggNOG	fuNOG05192.
GeneTree	EFGT00050000006352.
HOGENOM	HBG319770.
OMA	LQNPPGL.
OrthoDB	EOG4WWVT5.
Gene expression databases
ArrayExpress	O13933.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
KO	K03842.
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG1_SCHPO

Accession

Primary (citable) accession number: O13933

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program