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Protein

Chitobiosyldiphosphodolichol beta-mannosyltransferase

Gene

alg1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER (By similarity).By similarity

Catalytic activityi

GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT33. Glycosyltransferase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase (EC:2.4.1.142)
Alternative name(s):
Asparagine-linked glycosylation protein 1
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
Gene namesi
Name:alg1
ORF Names:SPAC23C4.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23C4.14.
PomBaseiSPAC23C4.14. alg1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2424Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini25 – 424400LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Chitobiosyldiphosphodolichol beta-mannosyltransferasePRO_0000080257Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO13933.

Interactioni

Protein-protein interaction databases

MINTiMINT-4669113.

Structurei

3D structure databases

ProteinModelPortaliO13933.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000216716.
InParanoidiO13933.
KOiK03842.
OMAiPKKRNAC.
OrthoDBiEOG72892M.
PhylomeDBiO13933.

Family and domain databases

InterProiIPR026051. ALG1-like.
[Graphical view]
PANTHERiPTHR13036. PTHR13036. 1 hit.

Sequencei

Sequence statusi: Complete.

O13933-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVLKIVLFL SLVIWFNLKK RTDKKRIIVL VLGDIARSPR MQYHAVSFAK
60 70 80 90 100
LGWKVDLLGY QHPGSSVGLF ESHENIRFYP IPSLPAYLQP KNRLQFLFLG
110 120 130 140 150
PLKVLHQFLA LNWALFVRKP ASFLFIQNPP CIPVFFIAQC LHILRGTKFI
160 170 180 190 200
IDWHNFGYSI LALKLGKQHT FVKLLKIYEK YMARGAYAHL TVSKRMKDVL
210 220 230 240 250
QTWGMNPCYV CYDRPPNHFT PIKNEQKKQM SIKKIPCEYN PSSTKLLITS
260 270 280 290 300
TSWTPDEDIY ILWEALNEYD KTLDTPKLLV LITGKGPMKE EFSQYIKKHP
310 320 330 340 350
LHKVRFCMPW LSIEDYPQVM ACADLGVCLH TSSSGLDLPM KVVDLFGCGV
360 370 380 390 400
PVIALSYPTI SELVHDGENG LIVNDSKALS KKMQYLLTHA NELNSLKLGA
410 420
LKESEYRWDD EWNKVIPPIV QGSN
Length:424
Mass (Da):48,726
Last modified:May 1, 2000 - v2
Checksum:iE652399C61A28579
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16885.2.
PIRiT38269.
RefSeqiNP_593186.1. NM_001018582.2.

Genome annotation databases

EnsemblFungiiSPAC23C4.14.1; SPAC23C4.14.1:pep; SPAC23C4.14.
GeneIDi2541937.
KEGGispo:SPAC23C4.14.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB16885.2.
PIRiT38269.
RefSeqiNP_593186.1. NM_001018582.2.

3D structure databases

ProteinModelPortaliO13933.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4669113.

Protein family/group databases

CAZyiGT33. Glycosyltransferase Family 33.

Proteomic databases

MaxQBiO13933.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC23C4.14.1; SPAC23C4.14.1:pep; SPAC23C4.14.
GeneIDi2541937.
KEGGispo:SPAC23C4.14.

Organism-specific databases

EuPathDBiFungiDB:SPAC23C4.14.
PomBaseiSPAC23C4.14. alg1.

Phylogenomic databases

HOGENOMiHOG000216716.
InParanoidiO13933.
KOiK03842.
OMAiPKKRNAC.
OrthoDBiEOG72892M.
PhylomeDBiO13933.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiO13933.

Family and domain databases

InterProiIPR026051. ALG1-like.
[Graphical view]
PANTHERiPTHR13036. PTHR13036. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiALG1_SCHPO
AccessioniPrimary (citable) accession number: O13933
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.