Reviewed, UniProtKB/Swiss-Prot O13919 (PST2_SCHPO)

Names and origin

Protein names

Recommended name:
    Paired amphipathic helix protein pst2
Alternative name(s):
    SIN3 homolog 2

Gene names

Name:	pst2
ORF Names:	SPAC23C11.15

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1075 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a role in chromatin assembly and chromosome segregation. Involved in the deacetylation of histones. Ref.3
Subunit structure	Heterotetramer of alp13, clr6, prw1 and pst2. Ref.3
Subcellular location	Nucleus. Ref.3
Sequence similarities	Contains 3 PAH (paired amphipathic helix) repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	chromatin remodeling Inferred by curator. Source: GeneDB_SPombe chromosome condensation Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe histone deacetylation Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Rpd3S complex Ref.3 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
alp13	O13953	1	EBI-904686,EBI-904711
clr6	O59702	1	EBI-904686,EBI-904651
prw1	O14021	1	EBI-904686,EBI-904698

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1075	1075	Paired amphipathic helix protein pst2		PRO_0000121542
Regions
	Repeat	54 – 100	47	PAH 1
	Repeat	160 – 206	47	PAH 2
	Repeat	271 – 317	47	PAH 3
Amino acid modifications
	Modified residue	641	1	Phosphoserine Ref.4
	Modified residue	643	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O13919-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1AD301DB4EB1AFFB	FASTA	1,075	124,849
	10 20 30 40 50 60 MEQTLAILKN DNSTLVAEMQ NQLVHDFSPN GTALPELDIK AFVQKLGQRL CHRPYVYSAF 70 80 90 100 110 120 MDVVKALHNE IVDFPGFIER ISVILRDYPD LLEYLNIFLP SSYKYLLSNS GANFTLQFTT 130 140 150 160 170 180 PSGPVSTPST YVATYNDLPC TYHRAIGFVS RVRRALLSNP EQFFKLQDSL RKFKNSECSL 190 200 210 220 230 240 SELQTIVTSL LAEHPSLAHE FHNFLPSSIF FGSKPPLGSF PLRGIQSSQF TLSNISDLLS 250 260 270 280 290 300 QSRPDNLSPF SHLSNESSDF FKNVKNVLTD VETYHEFLKL LNLYVQGIID RNILVSRGFG 310 320 330 340 350 360 FLKSNSGLWR SFLSLTSLSP EEFLSVYNSA CSDFPECGPS YRLLPVEERN ISCSGRDDFA 370 380 390 400 410 420 WGILNDDWVS HPTWASEESG FIVQRKTPYE EAMTKLEEER YEFDRHIEAT SWTIKSLKKI 430 440 450 460 470 480 QNRINELPEE ERETYTLEEG LGLPSKSIYK KTIKLVYTSE HAEEMFKALE RMPCLTLPLV 490 500 510 520 530 540 ISRLEEKNEE WKSVKRSLQP GWRSIEFKNY DKSLDSQCVY FKARDKKNVS SKFLLAEADI 550 560 570 580 590 600 LRSQAKLHFP LRSRSAFEFS FVYDNEIVLF DTCYMVCTYI VCNSPSGLKK VEHFFKNILP 610 620 630 640 650 660 LHFGLEKDKF SIFLDQVFRG PDYDVNAPNI VGNKPVRRKR SNSITQLTEF VKQPKINGQR 670 680 690 700 710 720 ESRSAAAARK KEESGNKSQS NSQNSLSDES GNVTPVSKKQ LSQPAAAIKA SLKYPSHPDS 730 740 750 760 770 780 LLEHQDHAGD TENEMHDDVD KEQFGYSSMY VFFRLFNLLY ERLYELQRLE DQVSIIQQRI 790 800 810 820 830 840 IPNPVSQKQK IWRDRWNDLS DVPDEKTHYE NTYVMILRLI YGIVDQSAFE DYLRFYYGNK 850 860 870 880 890 900 AYKIYTIDKL VWSAAKQVHH IVSDGKYKFV TSLVEQNSSA SPKKNYDDFL YRLEIEKLLN 910 920 930 940 950 960 PDEILFRFCW INKFKSFGIK IMKRANLIVD QSLDTQRRVW KKYVQNYRIQ KLTEEISYKN 970 980 990 1000 1010 1020 YRCPFLCRNI EKERTVEQLV SRLQTKLLRS AELVSGLQAK LCLDSFKLLY LPRTEDSYID 1030 1040 1050 1060 1070 ASYLRLRDTD FLDCQNKRKQ RWRNRWESLL KSVRGTSDNT AEVNFDADIN ALFIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"A new member of the Sin3 family of corepressors is essential for cell viability and required for retroelement propagation in fission yeast." Dang V.D., Benedik M.J., Ekwall K., Choi J., Allshire R.C., Levin H.L. Mol. Cell. Biol. 19:2351-2365(1999) [PubMed: 10022921] [Abstract] Cited for: GENE NAME.
[3]	"Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genomic integrity." Nakayama J., Xiao G., Noma K., Malikzay A., Bjerling P., Ekwall K., Kobayashi R., Grewal S.I.S. EMBO J. 22:2776-2787(2003) [PubMed: 12773392] [Abstract] Cited for: PROTEIN SEQUENCE OF 87-104; 280-291; 386-395; 424-446; 608-637; 678-709; 780-788; 819-834; 869-883; 925-937 AND 1055-1075, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641 AND SER-643, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB11171.1.
PIR	T38253.
RefSeq	NP_593646.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O13919. 3 interactions.
STRING	O13919.
Genome annotation databases
GeneID	2541624.
GenomeReviews	Gene locus pst2 in contig CU329670_GR.
KEGG	spo:SPAC23C11.15.
NMPDR	fig|4896.1.peg.3616.
Organism-specific databases
GeneDB_Spombe	SPAC23C11.15.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001315-MON.
Gene expression databases
ArrayExpress	O13919.
Family and domain databases
InterPro	IPR013194. HDAC_interact. IPR003822. PAH. [Graphical view]
Gene3D	G3DSA:1.20.1160.11. PAH. 2 hits.
Pfam	PF08295. HDAC_interact. 1 hit. PF02671. PAH. 3 hits. [Graphical view]
SMART	SM00761. HDAC_interact. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PST2_SCHPO

Accession

Primary (citable) accession number: O13919

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 1, 1998
Last modified:	September 22, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)