Reviewed, UniProtKB/Swiss-Prot O13914 (SYAC_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable alanyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

ORF Names:

SPAC23C11.09

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	959 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm Potential.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs Potential.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 959	959	Probable alanyl-tRNA synthetase, cytoplasmic		PRO_0000075286
Amino acid modifications
	Modified residue	389	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O13914-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9F2E4A7275D86FB6	FASTA	959	107,377
	10 20 30 40 50 60 MTAESEVVNW PANEIRRTFL KYFEDHGHTI VPSSSVIPYD DPTLLFANAG MNQFKPIFLG 70 80 90 100 110 120 TVDPSSDFAK LKRACDSQKC IRAGGKHNDL EDVGKDNYHH TMFEMLGNWS FGDYFKKEAI 130 140 150 160 170 180 AMAWDLLTNV YGLKKDQLYV TYFGGHEESG LEPDLEARQL WLDIGIDESR VIPGSLKDNF 190 200 210 220 230 240 WEMGDQGPCG PCSEIHYDRI GNRTVPELVN MDDPNVLEIW NIVFIQFNRE KDGSLRPLPN 250 260 270 280 290 300 RHVDTGMGFE RLVSVIQNKT SNYDTDVFSP IFAKIQELTN ARPYTGKMGD EDVDGIDTAY 310 320 330 340 350 360 RVVADHVRTL TFAISDGGVP NNEGRGYVLR RILRRGARYV RKKFGVPIGN FFSRLSLTVV 370 380 390 400 410 420 EQMGDFFPEL KRKVDDVREL LDEEEESFSR TLDRGEKMFE QYAAAAKKTP SKTLQGNDVW 430 440 450 460 470 480 RLYETYGFPV DLTHLMAEEA GIKIDEPGFE AAQARSKEIS KQASKGGSSG DDLLVLDVHA 490 500 510 520 530 540 LGALSKMDDI PETDDVFKHN SVSLKSVIKG IYHKGGFQKS TEGFNSGEQL GLLLDRTNFY 550 560 570 580 590 600 AEQGGQEYDT GHIVIDGVAD FRVTNVQVYA GYVLHTGFLE YGNLTVNDSV VCEYDEIRRW 610 620 630 640 650 660 HLMNNHTVTH ILNLALRNTL GDGIDQRGSL VSQEKLRFDF SYKSSIPIDK LLLVENYCNN 670 680 690 700 710 720 VIQDNLSVYS KEVALSKAKE INGLRAVFGE VYPDPVRVVC IGVDIDTLLQ EPKKPDWTKY 730 740 750 760 770 780 SIEFCGGTHC DKSGEIKDFV ILEENGIAKG IRRIVAVTST EANRVSRLAN EFDARIAKLE 790 800 810 820 830 840 KMPFSPAKEA ELKKISVDLS KLVVAAVRKH AMKERIAKIT KQVQEHVKAI NAAEQKEVVN 850 860 870 880 890 900 VVTEYFKENP DMSFVVAKVP ISANPKALSF ALTYAKKNLK DKSIYLLASD DTKVAHACLV 910 920 930 940 950 SPEAMKKLTP QEWSQKVCHS IGGRSGGKGD TCQGVGDKPL SIDVAVEEAI EFFQGKLTI

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-389, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB11162.1.
PIR	T38247.
RefSeq	NP_593640.1.
3D structure databases
SMR	O13914. Positions 11-464, 528-756.
ModBase	Search...
Protein-protein interaction databases
STRING	O13914.
Genome annotation databases
GeneID	2542031.
KEGG	spo:SPAC23C11.09.
NMPDR	fig|4896.1.peg.3610.
Organism-specific databases
GeneDB_Spombe	SPAC23C11.09.
Phylogenomic databases
eggNOG	fuNOG06017.
HOGENOM	HBG354397.
OMA	VILEMES.
OrthoDB	EOG9NP8KX.
PhylomeDB	O13914.
Enzyme and pathway databases
BRENDA	6.1.1.7. 653.
Gene expression databases
ArrayExpress	O13914.
Family and domain databases
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYAC_SCHPO

Accession

Primary (citable) accession number: O13914

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)