Reviewed, UniProtKB/Swiss-Prot O13911 (PNK1_SCHPO)

Names and origin

Protein names

Recommended name:
    Bifunctional polynucleotide phosphatase/kinase
Alternative name(s):
    Polynucleotide kinase-3'-phosphatase
    DNA 5'-kinase/3'-phosphatase
Including the following 2 domains:
    1- Recommended name:
            Polynucleotide 3'-phosphatase
              EC=3.1.3.32
        Alternative name(s):
            2'(3')-polynucleotidase
    2- Recommended name:
            Polynucleotide 5'-hydroxyl-kinase
              EC=2.7.1.78

Gene names

Name:	pnk1
ORF Names:	SPAC23C11.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Has a role in the repair of breaks in single stranded DNA. Ref.1
Catalytic activity	A 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate. ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.
Subcellular location	Nucleus Ref.1.
Sequence similarities	In the N-terminal section; belongs to the DNA 3' phosphatase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	nucleotide-excision repair, DNA damage removal Inferred from direct assay. Source: GeneDB_SPombe
Cellular component	nucleolus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Ref.1 Inferred from direct assay. Source: GeneDB_SPombe polynucleotide 3'-phosphatase activity Inferred from direct assay. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Bifunctional polynucleotide phosphatase/kinase		PRO_0000058477
Regions
	Nucleotide binding	263 – 270	8	ATP Potential
Amino acid modifications
	Modified residue	8	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O13911-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: FE9A732CD40E3146	FASTA	421	48,477
	10 20 30 40 50 60 MSSKKRKSPP QESLTSYFEK SSKSSKKYGS QNKDSDSSST CLQQKIEIQW SITDSLYIAK 70 80 90 100 110 120 YGKLKKTKKF IAFDLDGTLI KTKSGRVFSK DAADWTWWHP SVVPKLKALY QDNYSLVIFS 130 140 150 160 170 180 NQNGIPRKPS AGHTFQMKIR AIFESLDLPI VLYAAILKDK FRKPLTGMWN SFLKDVNRSI 190 200 210 220 230 240 DLSFIKYVGD AAGRPGDHNS TDLKFAENIG IKFETPEQFF LGHSFVPPNF ESFHPKNYLV 250 260 270 280 290 300 RNSSSHPYHF KKSEHQEIVV LVGFPSSGKS TLAESQIVTQ GYERVNQDIL KTKSKCIKAA 310 320 330 340 350 360 IEALKKEKSV VIGMYSIIST TYAISDNTNP TIESRKMWID IAQEFEIPIR CIHLQSSEEL 370 380 390 400 410 420 ARHNNVFRYI HHNQKQLPEI AFNSFKSRFQ MPTVEEGFTN VEEVPFKCLK DYEDTWNYWY E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pnk1, a DNA kinase/phosphatase required for normal response to DNA damage by gamma-radiation or camptothecin in Schizosaccharomyces pombe." Meijer M., Karimi-Busheri F., Huang T.Y., Weinfeld M., Young D. J. Biol. Chem. 277:4050-4055(2002) [PubMed: 11729194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB11157.1.
PIR	T38242.
RefSeq	NP_593635.1.
3D structure databases
SMR	O13911. Positions 48-416.
ModBase	Search...
Genome annotation databases
GeneID	2542032.
GenomeReviews	Gene locus pnk1 in contig CU329670_GR.
KEGG	spo:SPAC23C11.04c.
NMPDR	fig|4896.1.peg.3605.
Organism-specific databases
GeneDB_Spombe	SPAC23C11.04c.
Phylogenomic databases
eggNOG	fuNOG08837.
HOGENOM	HBG329365.
OMA	SWQRCVT.
OrthoDB	EOG9615MT.
PhylomeDB	O13911.
Enzyme and pathway databases
BRENDA	2.7.1.78. 653. 3.1.3.32. 653.
Gene expression databases
ArrayExpress	O13911.
Family and domain databases
InterPro	IPR006551. DNA-3-Pase. IPR006549. HAD-SF_hydro_IIIA. IPR015636. PNK_3Pase. [Graphical view]
PANTHER	PTHR12083. PNK_3Pase. 1 hit.
TIGRFAMs	TIGR01664. DNA-3_-Pase. 1 hit. TIGR01662. HAD-SF-IIIA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNK1_SCHPO

Accession

Primary (citable) accession number: O13911

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)