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Protein

Bifunctional polynucleotide phosphatase/kinase

Gene

pnk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Has a role in the repair of breaks in single-stranded DNA.1 Publication

Catalytic activityi

A 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi263 – 270ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: PomBase
  • DNA 3' dephosphorylation involved in DNA repair Source: PomBase
  • single strand break repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.1.3.32. 5613.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional polynucleotide phosphatase/kinase
Alternative name(s):
DNA 5'-kinase/3'-phosphatase
Polynucleotide kinase-3'-phosphatase
Including the following 2 domains:
Polynucleotide 3'-phosphatase (EC:3.1.3.32)
Alternative name(s):
2'(3')-polynucleotidase
Polynucleotide 5'-hydroxyl-kinase (EC:2.7.1.78)
Gene namesi
Name:pnk1
ORF Names:SPAC23C11.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23C11.04c.
PomBaseiSPAC23C11.04c. pnk1.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000584771 – 408Bifunctional polynucleotide phosphatase/kinaseAdd BLAST408

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO13911.
PRIDEiO13911.

PTM databases

iPTMnetiO13911.

Interactioni

Protein-protein interaction databases

BioGridi278514. 177 interactors.
MINTiMINT-4668916.

Structurei

3D structure databases

ProteinModelPortaliO13911.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the DNA 3' phosphatase family.Curated

Phylogenomic databases

HOGENOMiHOG000182000.
InParanoidiO13911.
KOiK08073.
OMAiDIYRKPR.
OrthoDBiEOG092C28JS.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006549. HAD-SF_hydro_IIIA.
IPR027417. P-loop_NTPase.
IPR013954. PNK3P.
IPR006551. Polynucleotide_phosphatase.
[Graphical view]
PfamiPF08645. PNK3P. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01664. DNA-3'-Pase. 1 hit.
TIGR01662. HAD-SF-IIIA. 1 hit.

Sequencei

Sequence statusi: Complete.

O13911-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKKRKSPP QESLTSYFEK SSKSSKKYGS QNKDSDSSST CLQQKIEIQW
60 70 80 90 100
SITDSLYIAK YGKLKKTKKF IAFDLDGTLI KTKSGRVFSK DAADWTWWHP
110 120 130 140 150
SVVPKLKALY QDNYSLVIFS NQNGIPRKPS AGHTFQMKIR AIFESLDLPI
160 170 180 190 200
VLYAAILKDK FRKPLTGMWN SFLKDVNRSI DLSFIKYVGD AAGRPGDHNS
210 220 230 240 250
TDLKFAENIG IKFETPEQFF LGHSFVPPNF ESFHPKNYLV RNSSSHPYHF
260 270 280 290 300
KKSEHQEIVV LVGFPSSGKS TLAESQIVTQ GYERVNQDIL KTKSKCIKAA
310 320 330 340 350
IEALKKEKSV VIDNTNPTIE SRKMWIDIAQ EFEIPIRCIH LQSSEELARH
360 370 380 390 400
NNVFRYIHHN QKQLPEIAFN SFKSRFQMPT VEEGFTNVEE VPFKCLKDYE

DTWNYWYE
Length:408
Mass (Da):47,089
Last modified:March 21, 2012 - v2
Checksum:iF4305563D5872634
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11157.2.
PIRiT38242.
RefSeqiNP_593635.2. NM_001019066.2.

Genome annotation databases

EnsemblFungiiSPAC23C11.04c.1; SPAC23C11.04c.1:pep; SPAC23C11.04c.
GeneIDi2542032.
KEGGispo:SPAC23C11.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11157.2.
PIRiT38242.
RefSeqiNP_593635.2. NM_001019066.2.

3D structure databases

ProteinModelPortaliO13911.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278514. 177 interactors.
MINTiMINT-4668916.

PTM databases

iPTMnetiO13911.

Proteomic databases

MaxQBiO13911.
PRIDEiO13911.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC23C11.04c.1; SPAC23C11.04c.1:pep; SPAC23C11.04c.
GeneIDi2542032.
KEGGispo:SPAC23C11.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAC23C11.04c.
PomBaseiSPAC23C11.04c. pnk1.

Phylogenomic databases

HOGENOMiHOG000182000.
InParanoidiO13911.
KOiK08073.
OMAiDIYRKPR.
OrthoDBiEOG092C28JS.

Enzyme and pathway databases

BRENDAi3.1.3.32. 5613.

Miscellaneous databases

PROiO13911.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006549. HAD-SF_hydro_IIIA.
IPR027417. P-loop_NTPase.
IPR013954. PNK3P.
IPR006551. Polynucleotide_phosphatase.
[Graphical view]
PfamiPF08645. PNK3P. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01664. DNA-3'-Pase. 1 hit.
TIGR01662. HAD-SF-IIIA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPNK1_SCHPO
AccessioniPrimary (citable) accession number: O13911
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 21, 2012
Last modified: October 5, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.