O13902 (DAK1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxyacetone kinase 1
Short name=DHA kinase 1
EC=2.7.1.29

Alternative name(s):
Glycerone kinase 1

Gene names

Name:	dak1
ORF Names:	SPAC22A12.11

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + glycerone = ADP + glycerone phosphate.
Pathway	Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.
Sequence similarities	Belongs to the dihydroxyacetone kinase (DAK) family. Contains 1 DhaK domain. Contains 1 DhaL domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerone kinase activity Inferred from direct assay. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Dihydroxyacetone kinase 1		PRO_0000121520
Regions
	Domain	8 – 349	342	DhaK
	Domain	384 – 580	197	DhaL
	Nucleotide binding	413 – 416	4	ATP By similarity
	Nucleotide binding	458 – 459	2	ATP By similarity
	Nucleotide binding	512 – 513	2	ATP By similarity
	Nucleotide binding	565 – 567	3	ATP By similarity
	Region	53 – 56	4	Substrate binding By similarity
	Compositional bias	161 – 166	6	Poly-Ala
Sites
	Active site	221	1	Tele-hemiaminal-histidine intermediate By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O13902 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8AC984318610E08D	FASTA	580	62,316
	10 20 30 40 50 60 MDKHFINDPE VLVLDGLKSL ADMNKTLTVH EEGKFIYFHD YNKKNVSVIS GGGAGHEPTH 70 80 90 100 110 120 SSFVGKGMLT AAVSGSIFAS PSSKQIYTGI KQVESEAGTL VICKNYTGDI LHFGMALEKQ 130 140 150 160 170 180 RTAGKKAELI AVADDVSVGR KKSGKVGRRG LSGTVLVHKI AGAAAARGLP LEAVTTIAKA 190 200 210 220 230 240 AIDNLVSIGA SLAHVHVPGH EPIAKEDEMK HDEMELGMGI HNEPGCKRIS PIPSIDDLIA 250 260 270 280 290 300 QMLKQMLDQS DKDRAYVKIE GDDEVVLLMN NLGGLSMLEF SAISHKVKEA LAKEYKINPV 310 320 330 340 350 360 RIFAGPFTTS LNGLGFGITL LRTTDRVKVE GEEYSLVDLI DQPVEAIGWP LCQPSDLKSK 370 380 390 400 410 420 NKIGNVSIEE GQKDVKSPVT VDKEKVRQAI VNSMENLIKA EPKITKFDTM AGDGDCGTTL 430 440 450 460 470 480 KRGAEGVLKF VKSDKFSDDP IRIVRDIADV IEDNMDGTSG ALYAIFFHGF AKGMKDTLEK 490 500 510 520 530 540 SKDISSKTWA AGLKVALDTL FKYTPARPGD STMCDALVPF VETFVKTNDL NAAVEEARKG 550 560 570 580 ADATADMQAK LGRAVYVGDD VKVPDAGALG VVAIVEGFTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and overexpression in Escherichia coli of the gene encoding dihydroxyacetone kinase isoenzyme I from Schizosaccharomyces pombe, and its application to dihydroxyacetone phosphate production." Itoh N., Tujibata Y., Liu J.Q. Appl. Microbiol. Biotechnol. 51:193-200(1999) [PubMed: 10091325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IFO 0354.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB010078 Genomic DNA. Translation: BAA24186.1. CU329670 Genomic DNA. Translation: CAB16581.1.
PIR	T43310.
RefSeq	NP_593241.1. NM_001018638.1.
3D structure databases
ProteinModelPortal	O13902.
ModBase	Search...
Protein-protein interaction databases
STRING	O13902.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC22A12.11.1; SPAC22A12.11.1:pep; SPAC22A12.11.
GeneID	2541809.
GenomeReviews	Gene locus dak1 in contig CU329670_GR.
KEGG	spo:SPAC22A12.11.
NMPDR	fig|4896.1.peg.3211.
Organism-specific databases
GeneDB_Spombe	SPAC22A12.11.
Phylogenomic databases
eggNOG	fuNOG05468.
GeneTree	EFGT00050000006766.
HOGENOM	HBG473357.
OMA	SHTASIG.
OrthoDB	EOG46T68Z.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000934-MONOMER.
BRENDA	2.7.1.29. 5615.
Gene expression databases
ArrayExpress	O13902.
Family and domain databases
InterPro	IPR004006. Dak1. IPR004007. Dak2. IPR012734. DhaK_ATP. [Graphical view]
KO	K00863.
Pfam	PF02733. Dak1. 1 hit. PF02734. Dak2. 1 hit. [Graphical view]
SUPFAM	SSF101473. Dak2. 1 hit.
TIGRFAMs	TIGR02361. Dak_ATP. 1 hit.
PROSITE	PS51481. DHAK. 1 hit. PS51480. DHAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAK1_SCHPO

Accession

Primary (citable) accession number: O13902

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program