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Protein

Importin subunit beta-1

Gene

kap95

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the clasical nuclear localization signal (cNLS) in cargo substrates. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, GTP-Ran binds to importin beta and the three components separate, leading to release of the cargo. Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-SPO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-1By similarity
Alternative name(s):
Importin-95By similarity
Karyopherin subunit beta-1By similarity
Karyopherin-95By similarity
Gene namesi
Name:kap95By similarity
ORF Names:SPAC1B1.03cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1B1.03c.
PomBaseiSPAC1B1.03c. kap95.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • mitotic spindle midzone Source: PomBase
  • nuclear envelope Source: PomBase
  • nuclear membrane Source: GO_Central
  • nuclear periphery Source: PomBase
  • nuclear pore Source: PomBase
  • protein complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

Pathology & Biotechi

Disruption phenotypei

Essential for growth. Spores germinate, but fail to divide.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207631 – 863Importin subunit beta-1Add BLAST863

Proteomic databases

MaxQBiO13864.
PRIDEiO13864.

Interactioni

Subunit structurei

Forms a complex with an importin alpha subunit. Interacts with Ran; interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange. Interacts with nucleoporins.By similarity

Protein-protein interaction databases

BioGridi278955. 2 interactors.
MINTiMINT-4668596.

Structurei

3D structure databases

ProteinModelPortaliO13864.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati2 – 31HEAT 1By similarityAdd BLAST30
Domaini21 – 101Importin N-terminalPROSITE-ProRule annotationAdd BLAST81
Repeati33 – 62HEAT 2By similarityAdd BLAST30
Repeati85 – 124HEAT 3By similarityAdd BLAST40
Repeati129 – 159HEAT 4By similarityAdd BLAST31
Repeati170 – 201HEAT 5By similarityAdd BLAST32
Repeati212 – 248HEAT 6By similarityAdd BLAST37
Repeati253 – 299HEAT 7By similarityAdd BLAST47
Repeati314 – 360HEAT 8By similarityAdd BLAST47
Repeati364 – 392HEAT 9By similarityAdd BLAST29
Repeati399 – 439HEAT 10By similarityAdd BLAST41
Repeati449 – 481HEAT 11By similarityAdd BLAST33
Repeati496 – 530HEAT 12By similarityAdd BLAST35
Repeati536 – 586HEAT 13By similarityAdd BLAST51
Repeati592 – 630HEAT 14By similarityAdd BLAST39
Repeati635 – 671HEAT 15By similarityAdd BLAST37
Repeati677 – 715HEAT 16By similarityAdd BLAST39
Repeati720 – 767HEAT 17By similarityAdd BLAST48
Repeati778 – 815HEAT 18By similarityAdd BLAST38
Repeati822 – 861HEAT 19By similarityAdd BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi315 – 320Poly-Ala6

Sequence similaritiesi

Contains 19 HEAT repeats.By similarity
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000204108.
InParanoidiO13864.
KOiK14293.
OMAiMSNILPY.
OrthoDBiEOG092C12XO.
PhylomeDBiO13864.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 4 hits.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAGEFLAQT LSPDANVRLN AEKQLENAAR TDFAQYMVLL AQELANDNSM
60 70 80 90 100
PYIRMAAGLA LKNAITAREE ARKLEYQQLW QSLPVEIKQQ VKSLALQTLG
110 120 130 140 150
SSEHQAGQSA AQLVAAIAAY ELATNQWPDL MVTLVANVGE GQPSALKQHS
160 170 180 190 200
LQTIGYICES VSPEVLSAQS NAILTAVVAG ARKEEPDAAV RLAALGALYD
210 220 230 240 250
SLEFVRENFN NEYERNYIMQ VVCEATQSPE ASIQTAAFGC LVKIMHLYYD
260 270 280 290 300
TMPFYMEKAL FALTTQGMYN TNEQVALQAV EFWSTVCEEE IEVNLEIQEA
310 320 330 340 350
QDLNEVPARQ NHGFARAAAA DILPVLLKLL CNQDEDADED DWNISMAAAT
360 370 380 390 400
CLQLFAQVVG DLIVNPVLAF VEQNIQNPDW HQREAAVMAF GSVLEGPNVA
410 420 430 440 450
MLTPLVNQAL PVLINMMVDP VIFVKDTTAW ALGQISSFVA DAINPEIHLS
460 470 480 490 500
PMVSALLQGL TDNPRIVANC CWAFMNLVCH FAPVDNHQTS VMTPFYEAII
510 520 530 540 550
GSLLHVTDQK GNENNSRTSG YETLGTLITF SSDSVLPMIA NVLSIILTRL
560 570 580 590 600
ETSIQMQSQI LDVEDRANHD ELQSNLCNVL TSIIRRFGPD IRTSSDQIMN
610 620 630 640 650
LLLQTMQTAP KQSVVHEDVL LAIGAMMNSL EEQFEVYVPS FVPFLSSALS
660 670 680 690 700
NEQEYQLCSV AVGLVGDLAR ALNAKILPYC DDFMTRLVQD LQSSVLDRNV
710 720 730 740 750
KPAILSCFSD IALAIGAAFQ TYLEAVMVLL QQASSVQAPP GANFSMIDYV
760 770 780 790 800
DALRLGIVEA YVGITQAVRT DNRLDLIQPY VHSMFTLLNM ITADPECSES
810 820 830 840 850
LTRAALGLLG DLAESFPKGE LKSYFAADWV AALLNSGKTK ISSQQTKDLA
860
RWATEQVKRQ ARA
Length:863
Mass (Da):94,747
Last modified:January 1, 1998 - v1
Checksum:i9E02D72F5A1C6839
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11082.1.
PIRiT38016.
RefSeqiNP_594233.1. NM_001019656.2.

Genome annotation databases

EnsemblFungiiSPAC1B1.03c.1; SPAC1B1.03c.1:pep; SPAC1B1.03c.
GeneIDi2542496.
KEGGispo:SPAC1B1.03c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11082.1.
PIRiT38016.
RefSeqiNP_594233.1. NM_001019656.2.

3D structure databases

ProteinModelPortaliO13864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278955. 2 interactors.
MINTiMINT-4668596.

Proteomic databases

MaxQBiO13864.
PRIDEiO13864.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1B1.03c.1; SPAC1B1.03c.1:pep; SPAC1B1.03c.
GeneIDi2542496.
KEGGispo:SPAC1B1.03c.

Organism-specific databases

EuPathDBiFungiDB:SPAC1B1.03c.
PomBaseiSPAC1B1.03c. kap95.

Phylogenomic databases

HOGENOMiHOG000204108.
InParanoidiO13864.
KOiK14293.
OMAiMSNILPY.
OrthoDBiEOG092C12XO.
PhylomeDBiO13864.

Enzyme and pathway databases

ReactomeiR-SPO-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiO13864.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 4 hits.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMB1_SCHPO
AccessioniPrimary (citable) accession number: O13864
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.