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O13853

- ITS3_SCHPO

UniProt

O13853 - ITS3_SCHPO

Protein

Phosphatidylinositol 4-phosphate 5-kinase its3

Gene

its3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 3 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Involved, together with the calcineurin ppb1, in cytokinesis.1 Publication

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: PomBase
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. diacylglycerol biosynthetic process Source: PomBase
    4. phosphatidylinositol metabolic process Source: PomBase
    5. phosphatidylinositol phosphorylation Source: GOC
    6. regulation of mitotic cytokinesis Source: PomBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_188312. Synthesis of PIPs at the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 4-phosphate 5-kinase its3 (EC:2.7.1.68)
    Alternative name(s):
    1-phosphatidylinositol 4-phosphate kinase
    Diphosphoinositide kinase
    PIP5K
    PtdIns(4)P-5-kinase
    Gene namesi
    Name:its3
    ORF Names:SPAC19G12.14
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC19G12.14.

    Subcellular locationi

    Nucleus. Membrane; Peripheral membrane protein
    Note: The active form of the enzyme is membrane-associated and concentrates at the septum in dividing cells.

    GO - Cellular componenti

    1. cell septum Source: PomBase
    2. nucleus Source: UniProtKB-SubCell
    3. plasma membrane Source: PomBase

    Keywords - Cellular componenti

    Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 742742Phosphatidylinositol 4-phosphate 5-kinase its3PRO_0000185484Add
    BLAST

    Post-translational modificationi

    Phosphorylated by casein kinase I. Phosphorylation inactivates the enzyme.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO13853.

    Interactioni

    Protein-protein interaction databases

    BioGridi278704. 13 interactions.
    STRINGi4896.SPAC19G12.14-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO13853.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini264 – 662399PIPKPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi97 – 218122Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PIPK domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5253.
    HOGENOMiHOG000193875.
    KOiK00889.
    OrthoDBiEOG7XSTPB.
    PhylomeDBiO13853.

    Family and domain databases

    Gene3Di3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProiIPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view]
    PANTHERiPTHR23086. PTHR23086. 1 hit.
    PfamiPF01504. PIP5K. 1 hit.
    [Graphical view]
    SMARTiSM00330. PIPKc. 1 hit.
    [Graphical view]
    PROSITEiPS51455. PIPK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O13853-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIDSNGIVN PHSITNEIPS YDEKQAVDLN GNAFAPNGTF QKKDLISHKN    50
    DFERTMRHDV LHTNPKIEVR SETHIYEPND SLFKENQDFP SNPTAHSPSS 100
    SSNDSVITAT GVPDGILRDS PIVSALEPPS SNSSSSPQLQ NLKHQLSSPQ 150
    PSRAPIDRSS SNPVTSSQQP PNDRSTLSSS QKAKRPLKRS YSEKNSSNAE 200
    PSGSRSGDRG TNVSTSGSLL DGIPPDIGSA SWAEAVKQKR VNMRRRREEL 250
    DDECVLVGTR VSEGHENYVT AYNMLTGIRV GVSRCQAKMD RELTPADFTA 300
    RHKFTFDITG NELTPSAKYD FKFKDYAPWV FRHLRQLFHL DAADYLVSLT 350
    SKYILSELDS PGKSGSFFYF SRDYRFIIKT IHHSEHKFLR EILYDYYEHV 400
    KNNPNTLISQ FYGLHRVKLP FGRKIHFVVM NNLFPPHRDI HQTFDLKGST 450
    LGRELDENQP CQSPMCTMKD TNWIRRNMHL QFGPLKRQIF LTQVKADIDM 500
    LSSLGIMDYS LLVGIHDLSR GNRDKIRNSI LSVYDPNVSQ HRVPSINGNE 550
    SHSNVHVIRQ VVNSTGPVSL DQSCNLLPTD QFVERRNFMF YSDDGGFQAT 600
    DENNEPGNFI FYIGIIDLLT KYSYVKRVEH LWKGINHSDS VISAVPPAEY 650
    ASRFYKFVES SIKPTLLVLK PFPLKPQDGQ RVNKQQSVNA GNVRTNNKHG 700
    SLNNNTAPSS RNAKSTSAHK SPKTEHRFPF PCRNVTTNTS SS 742
    Length:742
    Mass (Da):83,748
    Last modified:February 1, 2005 - v3
    Checksum:iF0A3A8C668318970
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB10125.1.
    AB027961 Genomic DNA. Translation: BAA87265.1.
    PIRiT38001.
    RefSeqiNP_594429.1. NM_001019858.2.

    Genome annotation databases

    EnsemblFungiiSPAC19G12.14.1; SPAC19G12.14.1:pep; SPAC19G12.14.
    GeneIDi2542231.
    KEGGispo:SPAC19G12.14.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB10125.1 .
    AB027961 Genomic DNA. Translation: BAA87265.1 .
    PIRi T38001.
    RefSeqi NP_594429.1. NM_001019858.2.

    3D structure databases

    ProteinModelPortali O13853.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 278704. 13 interactions.
    STRINGi 4896.SPAC19G12.14-1.

    Proteomic databases

    MaxQBi O13853.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC19G12.14.1 ; SPAC19G12.14.1:pep ; SPAC19G12.14 .
    GeneIDi 2542231.
    KEGGi spo:SPAC19G12.14.

    Organism-specific databases

    PomBasei SPAC19G12.14.

    Phylogenomic databases

    eggNOGi COG5253.
    HOGENOMi HOG000193875.
    KOi K00889.
    OrthoDBi EOG7XSTPB.
    PhylomeDBi O13853.

    Enzyme and pathway databases

    Reactomei REACT_188312. Synthesis of PIPs at the plasma membrane.

    Miscellaneous databases

    NextBioi 20803299.

    Family and domain databases

    Gene3Di 3.30.800.10. 1 hit.
    3.30.810.10. 2 hits.
    InterProi IPR023610. PInositol-4-P-5-kinase.
    IPR027483. PInositol-4-P-5-kinase_C.
    IPR002498. PInositol-4-P-5-kinase_core.
    IPR027484. PInositol-4-P-5-kinase_N.
    IPR016034. PInositol-4P-5-kinase_core_sub.
    [Graphical view ]
    PANTHERi PTHR23086. PTHR23086. 1 hit.
    Pfami PF01504. PIP5K. 1 hit.
    [Graphical view ]
    SMARTi SM00330. PIPKc. 1 hit.
    [Graphical view ]
    PROSITEi PS51455. PIPK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Phosphatidylinositol 4-phosphate 5-kinase Its3 and calcineurin Ppb1 coordinately regulate cytokinesis in fission yeast."
      Zhang Y., Sugiura R., Lu Y., Asami M., Maeda T., Itoh T., Takenawa T., Shuntoh H., Kuno T.
      J. Biol. Chem. 275:35600-35606(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
      Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
      Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 238-445, SUBCELLULAR LOCATION.
      Strain: ATCC 38364 / 968.
    4. "Regulation of phosphatidylinositol 4-phosphate 5-kinase from Schizosaccharomyces pombe by casein kinase I."
      Vancurova I., Choi J.H., Lin H., Kuret J., Vancura A.
      J. Biol. Chem. 274:1147-1155(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.

    Entry informationi

    Entry nameiITS3_SCHPO
    AccessioniPrimary (citable) accession number: O13853
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 99 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3