O13853 (ITS3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol-4-phosphate 5-kinase its3
EC=2.7.1.68

Alternative name(s):
1-phosphatidylinositol-4-phosphate kinase
Diphosphoinositide kinase
PIP5K
PtdIns(4)P-5-kinase

Gene names

Name:	its3
ORF Names:	SPAC19G12.14

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	742 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved, together with the calcineurin ppb1, in cytokinesis. Ref.1
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Subcellular location	Nucleus. Membrane; Peripheral membrane protein. Note: The active form of the enzyme is membrane-associated and concentrates at the septum in dividing cells. Ref.1 Ref.3 Ref.4
Post-translational modification	Phosphorylated by casein kinase I. Phosphorylation inactivates the enzyme. Ref.4
Sequence similarities	Contains 1 PIPK domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Membrane Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol biosynthetic process Inferred by curator. Source: GeneDB_Spombe regulation of cell cycle cytokinesis Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	cell septum Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	1-phosphatidylinositol-4-phosphate 5-kinase activity Inferred from genetic interaction. Source: GeneDB_Spombe ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 742	742	Phosphatidylinositol-4-phosphate 5-kinase its3		PRO_0000185484
Regions
	Domain	264 – 662	399	PIPK
	Compositional bias	97 – 218	122	Ser-rich

Sequences

	Sequence		Length	Mass (Da)
	O13853 [UniParc]. Last modified February 1, 2005. Version 3. Checksum: F0A3A8C668318970	FASTA	742	83,748
	10 20 30 40 50 60 MKIDSNGIVN PHSITNEIPS YDEKQAVDLN GNAFAPNGTF QKKDLISHKN DFERTMRHDV 70 80 90 100 110 120 LHTNPKIEVR SETHIYEPND SLFKENQDFP SNPTAHSPSS SSNDSVITAT GVPDGILRDS 130 140 150 160 170 180 PIVSALEPPS SNSSSSPQLQ NLKHQLSSPQ PSRAPIDRSS SNPVTSSQQP PNDRSTLSSS 190 200 210 220 230 240 QKAKRPLKRS YSEKNSSNAE PSGSRSGDRG TNVSTSGSLL DGIPPDIGSA SWAEAVKQKR 250 260 270 280 290 300 VNMRRRREEL DDECVLVGTR VSEGHENYVT AYNMLTGIRV GVSRCQAKMD RELTPADFTA 310 320 330 340 350 360 RHKFTFDITG NELTPSAKYD FKFKDYAPWV FRHLRQLFHL DAADYLVSLT SKYILSELDS 370 380 390 400 410 420 PGKSGSFFYF SRDYRFIIKT IHHSEHKFLR EILYDYYEHV KNNPNTLISQ FYGLHRVKLP 430 440 450 460 470 480 FGRKIHFVVM NNLFPPHRDI HQTFDLKGST LGRELDENQP CQSPMCTMKD TNWIRRNMHL 490 500 510 520 530 540 QFGPLKRQIF LTQVKADIDM LSSLGIMDYS LLVGIHDLSR GNRDKIRNSI LSVYDPNVSQ 550 560 570 580 590 600 HRVPSINGNE SHSNVHVIRQ VVNSTGPVSL DQSCNLLPTD QFVERRNFMF YSDDGGFQAT 610 620 630 640 650 660 DENNEPGNFI FYIGIIDLLT KYSYVKRVEH LWKGINHSDS VISAVPPAEY ASRFYKFVES 670 680 690 700 710 720 SIKPTLLVLK PFPLKPQDGQ RVNKQQSVNA GNVRTNNKHG SLNNNTAPSS RNAKSTSAHK 730 740 SPKTEHRFPF PCRNVTTNTS SS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phosphatidylinositol 4-phosphate 5-kinase Its3 and calcineurin Ppb1 coordinately regulate cytokinesis in fission yeast." Zhang Y., Sugiura R., Lu Y., Asami M., Maeda T., Itoh T., Takenawa T., Shuntoh H., Kuno T. J. Biol. Chem. 275:35600-35606(2000) [PubMed: 10950958] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 238-445, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[4]	"Regulation of phosphatidylinositol 4-phosphate 5-kinase from Schizosaccharomyces pombe by casein kinase I." Vancurova I., Choi J.H., Lin H., Kuret J., Vancura A. J. Biol. Chem. 274:1147-1155(1999) [PubMed: 9873063] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB10125.1. AB027961 Genomic DNA. Translation: BAA87265.1.
PIR	T38001.
RefSeq	NP_594429.1. NM_001019858.1.
3D structure databases
ProteinModelPortal	O13853.
ModBase	Search...
Protein-protein interaction databases
STRING	O13853.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC19G12.14.1; SPAC19G12.14.1:pep; SPAC19G12.14.
GeneID	2542231.
GenomeReviews	Gene locus its3 in contig CU329670_GR.
KEGG	spo:SPAC19G12.14.
NMPDR	fig|4896.1.peg.4399.
Organism-specific databases
GeneDB_Spombe	SPAC19G12.14.
Phylogenomic databases
eggNOG	fuNOG07600.
GeneTree	EFGT00050000002191.
OrthoDB	EOG4H49CG.
Gene expression databases
ArrayExpress	O13853.
Family and domain databases
InterPro	IPR023610. PInositol-4-P-5-kinase. IPR002498. PInositol-4-P-5-kinase_core. IPR016034. PInositol-4P-5-kinase_core_sub. [Graphical view]
KO	K00889.
PANTHER	PTHR23086. PIP5K. 1 hit.
Pfam	PF01504. PIP5K. 1 hit. [Graphical view]
SMART	SM00330. PIPKc. 1 hit. [Graphical view]
PROSITE	PS51455. PIPK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ITS3_SCHPO

Accession

Primary (citable) accession number: O13853

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	December 14, 2011
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program