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O13853 (ITS3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphatidylinositol 4-phosphate 5-kinase its3

EC=2.7.1.68
Alternative name(s):
1-phosphatidylinositol 4-phosphate kinase
Diphosphoinositide kinase
PIP5K
PtdIns(4)P-5-kinase
Gene names
Name:its3
ORF Names:SPAC19G12.14
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length742 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved, together with the calcineurin ppb1, in cytokinesis. Ref.1

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Subcellular location

Nucleus. Membrane; Peripheral membrane protein. Note: The active form of the enzyme is membrane-associated and concentrates at the septum in dividing cells. Ref.1 Ref.3 Ref.4

Post-translational modification

Phosphorylated by casein kinase I. Phosphorylation inactivates the enzyme. Ref.4

Sequence similarities

Contains 1 PIPK domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 742742Phosphatidylinositol 4-phosphate 5-kinase its3
PRO_0000185484

Regions

Domain264 – 662399PIPK
Compositional bias97 – 218122Ser-rich

Sequences

Sequence LengthMass (Da)Tools
O13853 [UniParc].

Last modified February 1, 2005. Version 3.
Checksum: F0A3A8C668318970

FASTA74283,748
        10         20         30         40         50         60 
MKIDSNGIVN PHSITNEIPS YDEKQAVDLN GNAFAPNGTF QKKDLISHKN DFERTMRHDV 

        70         80         90        100        110        120 
LHTNPKIEVR SETHIYEPND SLFKENQDFP SNPTAHSPSS SSNDSVITAT GVPDGILRDS 

       130        140        150        160        170        180 
PIVSALEPPS SNSSSSPQLQ NLKHQLSSPQ PSRAPIDRSS SNPVTSSQQP PNDRSTLSSS 

       190        200        210        220        230        240 
QKAKRPLKRS YSEKNSSNAE PSGSRSGDRG TNVSTSGSLL DGIPPDIGSA SWAEAVKQKR 

       250        260        270        280        290        300 
VNMRRRREEL DDECVLVGTR VSEGHENYVT AYNMLTGIRV GVSRCQAKMD RELTPADFTA 

       310        320        330        340        350        360 
RHKFTFDITG NELTPSAKYD FKFKDYAPWV FRHLRQLFHL DAADYLVSLT SKYILSELDS 

       370        380        390        400        410        420 
PGKSGSFFYF SRDYRFIIKT IHHSEHKFLR EILYDYYEHV KNNPNTLISQ FYGLHRVKLP 

       430        440        450        460        470        480 
FGRKIHFVVM NNLFPPHRDI HQTFDLKGST LGRELDENQP CQSPMCTMKD TNWIRRNMHL 

       490        500        510        520        530        540 
QFGPLKRQIF LTQVKADIDM LSSLGIMDYS LLVGIHDLSR GNRDKIRNSI LSVYDPNVSQ 

       550        560        570        580        590        600 
HRVPSINGNE SHSNVHVIRQ VVNSTGPVSL DQSCNLLPTD QFVERRNFMF YSDDGGFQAT 

       610        620        630        640        650        660 
DENNEPGNFI FYIGIIDLLT KYSYVKRVEH LWKGINHSDS VISAVPPAEY ASRFYKFVES 

       670        680        690        700        710        720 
SIKPTLLVLK PFPLKPQDGQ RVNKQQSVNA GNVRTNNKHG SLNNNTAPSS RNAKSTSAHK 

       730        740 
SPKTEHRFPF PCRNVTTNTS SS 

« Hide

References

« Hide 'large scale' references
[1]"Phosphatidylinositol 4-phosphate 5-kinase Its3 and calcineurin Ppb1 coordinately regulate cytokinesis in fission yeast."
Zhang Y., Sugiura R., Lu Y., Asami M., Maeda T., Itoh T., Takenawa T., Shuntoh H., Kuno T.
J. Biol. Chem. 275:35600-35606(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 238-445, SUBCELLULAR LOCATION.
Strain: ATCC 38364 / 968.
[4]"Regulation of phosphatidylinositol 4-phosphate 5-kinase from Schizosaccharomyces pombe by casein kinase I."
Vancurova I., Choi J.H., Lin H., Kuret J., Vancura A.
J. Biol. Chem. 274:1147-1155(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329670 Genomic DNA. Translation: CAB10125.1.
AB027961 Genomic DNA. Translation: BAA87265.1.
PIRT38001.
RefSeqNP_594429.1. NM_001019858.2.

3D structure databases

ProteinModelPortalO13853.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid278704. 13 interactions.
STRING4896.SPAC19G12.14-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC19G12.14.1; SPAC19G12.14.1:pep; SPAC19G12.14.
GeneID2542231.
KEGGspo:SPAC19G12.14.

Organism-specific databases

PomBaseSPAC19G12.14.

Phylogenomic databases

eggNOGCOG5253.
HOGENOMHOG000193875.
KOK00889.
OrthoDBEOG7XSTPB.
PhylomeDBO13853.

Family and domain databases

Gene3D3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
InterProIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERPTHR23086. PTHR23086. 1 hit.
PfamPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20803299.

Entry information

Entry nameITS3_SCHPO
AccessionPrimary (citable) accession number: O13853
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names