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O13849

- CBPY_SCHPO

UniProt

O13849 - CBPY_SCHPO

Protein

Carboxypeptidase Y

Gene

cpy1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.

    Catalytic activityi

    Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei715 – 7151PROSITE-ProRule annotation
    Active sitei921 – 9211PROSITE-ProRule annotation
    Binding sitei924 – 9241SubstrateBy similarity
    Active sitei978 – 9781PROSITE-ProRule annotation
    Binding sitei979 – 9791SubstrateBy similarity

    GO - Molecular functioni

    1. serine-type carboxypeptidase activity Source: PomBase

    GO - Biological processi

    1. protein catabolic process in the vacuole Source: PomBase

    Keywords - Molecular functioni

    Carboxypeptidase, Hydrolase, Protease

    Protein family/group databases

    MEROPSiS10.A66.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carboxypeptidase Y (EC:3.4.16.5)
    Short name:
    CPY
    Gene namesi
    Name:cpy1
    Synonyms:pcy1
    ORF Names:SPAC19G12.10c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC19G12.10c.

    Subcellular locationi

    Vacuole 1 Publication
    Note: Lysosome-like vacuoles.

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. fungal-type vacuole Source: PomBase

    Keywords - Cellular componenti

    Vacuole

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi627 – 6271C → T: 36% of original activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 521503Sequence AnalysisPRO_0000004295Add
    BLAST
    Chaini522 – 1002481Carboxypeptidase YPRO_0000004296Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi627 ↔ 880By similarity
    Glycosylationi659 – 6591N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi776 ↔ 789By similarity
    Disulfide bondi799 ↔ 822By similarity
    Disulfide bondi806 ↔ 815By similarity
    Disulfide bondi844 ↔ 851By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiO13849.
    PaxDbiO13849.

    Interactioni

    Subunit structurei

    Heterodimer of two subunits of 32 kDa and 19 kDa derived from the precursor protein and linked by a disulfide bond.1 Publication

    Protein-protein interaction databases

    BioGridi278926. 4 interactions.
    IntActiO13849. 1 interaction.
    MINTiMINT-4668478.
    STRINGi4896.SPAC19G12.10c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO13849.
    SMRiO13849. Positions 572-1000.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati225 – 237131-1Add
    BLAST
    Repeati238 – 250131-2Add
    BLAST
    Repeati251 – 263131-3Add
    BLAST
    Repeati264 – 276131-4Add
    BLAST
    Repeati277 – 289131-5Add
    BLAST
    Repeati290 – 302131-6Add
    BLAST
    Repeati303 – 315131-7Add
    BLAST
    Repeati316 – 328131-8Add
    BLAST
    Repeati329 – 341131-9Add
    BLAST
    Repeati361 – 36992-1
    Repeati370 – 37892-2
    Repeati379 – 38792-3
    Repeati388 – 39692-4
    Repeati397 – 40592-5
    Repeati406 – 41492-6
    Repeati415 – 42392-7; approximate

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni225 – 3411179 X 13 AA tandem repeats of M-H-H-E-P-G-E-H-M-P-P-P-PAdd
    BLAST
    Regioni361 – 423637 X 9 AA tandem repeats of D-K-E-H-H-K-G-P-KAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi208 – 341134Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S10 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG2939.
    KOiK13289.
    OMAiGEHMPPP.
    OrthoDBiEOG7XDBR1.

    Family and domain databases

    Gene3Di3.40.50.1820. 2 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view]
    PANTHERiPTHR11802. PTHR11802. 1 hit.
    PfamiPF00450. Peptidase_S10. 1 hit.
    [Graphical view]
    PRINTSiPR00724. CRBOXYPTASEC.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O13849-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLMKQTFLYF LLTCVVSAQF NGYVPPEQNG GDIVVPKDFY EKFGEDFIRE     50
    QEESSAPLMN PVPERDEAEA PHHPKGHHEF NDDFEDDTAL EHPGFKDKLD 100
    SFLQPARDFL HTVSDRLDNI FDDDEDEHVR EKRPHDSADE DAPRRKHGKC 150
    KGKGKHHKGK HAKGKGKKSH PKPEDDSVFF DDERPKHHEF DDEDREFPAH 200
    HEPGEHMPPP PMHHKPGEHM PPPPMHHEPG EHMPPPPMHH EPGEHMPPPP 250
    MHHEPGEHMP PPPMHHEPGE HMPPPPMHHE PGEHMPPPPM HHEPGEHMPP 300
    PPMHHEPGEH MPPPPMHHEP GEHMPPPPMH HEPGEHMPPP PFKHHELEEH 350
    EGPEHHRGPE DKEHHKGPKD KEHHKGPKDK EHHKGPKDKE HHKGPKDKEH 400
    HKGPKDKEHH KGPKDKEHHQ GPKEKHNERP EQNMQSSHEL LVIEAFADLI 450
    NSVPVEEIAE EFSRFLDTLG IEYYGNIPVH IQENAPKDSS IPPLFEFDDD 500
    LELSDLTPEQ FAYLEMLKAE GIDPMTAFRD QSHPAKPSNA QPADSSRPYA 550
    VFSQEENGEH VNLKAFPDHT LRVKDSKPES LGIDTVKQYT GYLDVEDDRH 600
    LFFWFFESRN DPENDPVVLW LNGGPGCSSL TGLFMELGPS SINIETLKPE 650
    YNPHSWNSNA SVIFLDQPIN TGFSNGDDSV LDTVTAGKDV YAFLNLFFAK 700
    FPQYAHLDFH IAGESYAGHY IPQFAKEIME HNQGANFFVA SGYEMEKQYI 750
    NLKSVLIGNG LTDPLVQYYF YGKMACESPY GPIMSQEECD RITGAYDTCA 800
    KLITGCYQTG FTPVCIGASL YCNNAMIGPF TKTGLNIYDI REECRDQEHL 850
    CYPETGAIES YLNQEFVQEA LGVEYDYKGC NTEVNIGFLF KGDWMRKTFR 900
    DDVTAILEAG LPVLIYAGDA DYICNYMGNE AWTDALEWAG QREFYEAELK 950
    PWSPNGKEAG RGKSFKNFGY LRLYEAGHMV PFNQPEASLE MLNSWIDGSL 1000
    FA 1002
    Length:1,002
    Mass (Da):114,237
    Last modified:January 1, 1998 - v1
    Checksum:i4A8D81CFDAB2D854
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86560 Genomic DNA. Translation: BAA25568.1.
    CU329670 Genomic DNA. Translation: CAB10121.1.
    PIRiT43236.
    RefSeqiNP_594425.1. NM_001019854.2.

    Genome annotation databases

    EnsemblFungiiSPAC19G12.10c.1; SPAC19G12.10c.1:pep; SPAC19G12.10c.
    GeneIDi2542465.
    KEGGispo:SPAC19G12.10c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86560 Genomic DNA. Translation: BAA25568.1 .
    CU329670 Genomic DNA. Translation: CAB10121.1 .
    PIRi T43236.
    RefSeqi NP_594425.1. NM_001019854.2.

    3D structure databases

    ProteinModelPortali O13849.
    SMRi O13849. Positions 572-1000.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 278926. 4 interactions.
    IntActi O13849. 1 interaction.
    MINTi MINT-4668478.
    STRINGi 4896.SPAC19G12.10c-1.

    Protein family/group databases

    MEROPSi S10.A66.

    Proteomic databases

    MaxQBi O13849.
    PaxDbi O13849.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC19G12.10c.1 ; SPAC19G12.10c.1:pep ; SPAC19G12.10c .
    GeneIDi 2542465.
    KEGGi spo:SPAC19G12.10c.

    Organism-specific databases

    PomBasei SPAC19G12.10c.

    Phylogenomic databases

    eggNOGi COG2939.
    KOi K13289.
    OMAi GEHMPPP.
    OrthoDBi EOG7XDBR1.

    Miscellaneous databases

    NextBioi 20803521.

    Family and domain databases

    Gene3Di 3.40.50.1820. 2 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view ]
    PANTHERi PTHR11802. PTHR11802. 1 hit.
    Pfami PF00450. Peptidase_S10. 1 hit.
    [Graphical view ]
    PRINTSi PR00724. CRBOXYPTASEC.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vacuolar protein sorting in fission yeast: cloning, biosynthesis, transport, and processing of carboxypeptidase Y from Schizosaccharomyces pombe."
      Tabuchi M., Iwaihara O., Ohtani Y., Ohuchi N., Sakurai J., Morita T., Iwahara S., Takegawa K.
      J. Bacteriol. 179:4179-4189(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-627.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiCBPY_SCHPO
    AccessioniPrimary (citable) accession number: O13849
    Secondary accession number(s): O14366
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3