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O13849

- CBPY_SCHPO

UniProt

O13849 - CBPY_SCHPO

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Protein

Carboxypeptidase Y

Gene
cpy1, pcy1, SPAC19G12.10c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei715 – 7151 By similarity
Active sitei921 – 9211 By similarity
Binding sitei924 – 9241Substrate By similarity
Active sitei978 – 9781 By similarity
Binding sitei979 – 9791Substrate By similarity

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: PomBase

GO - Biological processi

  1. vacuolar protein catabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Protein family/group databases

MEROPSiS10.A66.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase Y (EC:3.4.16.5)
Short name:
CPY
Gene namesi
Name:cpy1
Synonyms:pcy1
ORF Names:SPAC19G12.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC19G12.10c.

Subcellular locationi

Vacuole
Note: Lysosome-like vacuoles.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. fungal-type vacuole Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi627 – 6271C → T: 36% of original activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed predictionAdd
BLAST
Propeptidei19 – 521503 Reviewed predictionPRO_0000004295Add
BLAST
Chaini522 – 1002481Carboxypeptidase YPRO_0000004296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi627 ↔ 880 By similarity
Glycosylationi659 – 6591N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi776 ↔ 789 By similarity
Disulfide bondi799 ↔ 822 By similarity
Disulfide bondi806 ↔ 815 By similarity
Disulfide bondi844 ↔ 851 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiO13849.
PaxDbiO13849.

Interactioni

Subunit structurei

Heterodimer of two subunits of 32 kDa and 19 kDa derived from the precursor protein and linked by a disulfide bond.1 Publication

Protein-protein interaction databases

BioGridi278926. 4 interactions.
IntActiO13849. 1 interaction.
MINTiMINT-4668478.
STRINGi4896.SPAC19G12.10c-1.

Structurei

3D structure databases

ProteinModelPortaliO13849.
SMRiO13849. Positions 572-1000.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati225 – 237131-1Add
BLAST
Repeati238 – 250131-2Add
BLAST
Repeati251 – 263131-3Add
BLAST
Repeati264 – 276131-4Add
BLAST
Repeati277 – 289131-5Add
BLAST
Repeati290 – 302131-6Add
BLAST
Repeati303 – 315131-7Add
BLAST
Repeati316 – 328131-8Add
BLAST
Repeati329 – 341131-9Add
BLAST
Repeati361 – 36992-1
Repeati370 – 37892-2
Repeati379 – 38792-3
Repeati388 – 39692-4
Repeati397 – 40592-5
Repeati406 – 41492-6
Repeati415 – 42392-7; approximate

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 3411179 X 13 AA tandem repeats of M-H-H-E-P-G-E-H-M-P-P-P-PAdd
BLAST
Regioni361 – 423637 X 9 AA tandem repeats of D-K-E-H-H-K-G-P-KAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi208 – 341134Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S10 family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2939.
KOiK13289.
OMAiGEHMPPP.
OrthoDBiEOG7XDBR1.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13849-1 [UniParc]FASTAAdd to Basket

« Hide

MLMKQTFLYF LLTCVVSAQF NGYVPPEQNG GDIVVPKDFY EKFGEDFIRE     50
QEESSAPLMN PVPERDEAEA PHHPKGHHEF NDDFEDDTAL EHPGFKDKLD 100
SFLQPARDFL HTVSDRLDNI FDDDEDEHVR EKRPHDSADE DAPRRKHGKC 150
KGKGKHHKGK HAKGKGKKSH PKPEDDSVFF DDERPKHHEF DDEDREFPAH 200
HEPGEHMPPP PMHHKPGEHM PPPPMHHEPG EHMPPPPMHH EPGEHMPPPP 250
MHHEPGEHMP PPPMHHEPGE HMPPPPMHHE PGEHMPPPPM HHEPGEHMPP 300
PPMHHEPGEH MPPPPMHHEP GEHMPPPPMH HEPGEHMPPP PFKHHELEEH 350
EGPEHHRGPE DKEHHKGPKD KEHHKGPKDK EHHKGPKDKE HHKGPKDKEH 400
HKGPKDKEHH KGPKDKEHHQ GPKEKHNERP EQNMQSSHEL LVIEAFADLI 450
NSVPVEEIAE EFSRFLDTLG IEYYGNIPVH IQENAPKDSS IPPLFEFDDD 500
LELSDLTPEQ FAYLEMLKAE GIDPMTAFRD QSHPAKPSNA QPADSSRPYA 550
VFSQEENGEH VNLKAFPDHT LRVKDSKPES LGIDTVKQYT GYLDVEDDRH 600
LFFWFFESRN DPENDPVVLW LNGGPGCSSL TGLFMELGPS SINIETLKPE 650
YNPHSWNSNA SVIFLDQPIN TGFSNGDDSV LDTVTAGKDV YAFLNLFFAK 700
FPQYAHLDFH IAGESYAGHY IPQFAKEIME HNQGANFFVA SGYEMEKQYI 750
NLKSVLIGNG LTDPLVQYYF YGKMACESPY GPIMSQEECD RITGAYDTCA 800
KLITGCYQTG FTPVCIGASL YCNNAMIGPF TKTGLNIYDI REECRDQEHL 850
CYPETGAIES YLNQEFVQEA LGVEYDYKGC NTEVNIGFLF KGDWMRKTFR 900
DDVTAILEAG LPVLIYAGDA DYICNYMGNE AWTDALEWAG QREFYEAELK 950
PWSPNGKEAG RGKSFKNFGY LRLYEAGHMV PFNQPEASLE MLNSWIDGSL 1000
FA 1002
Length:1,002
Mass (Da):114,237
Last modified:January 1, 1998 - v1
Checksum:i4A8D81CFDAB2D854
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86560 Genomic DNA. Translation: BAA25568.1.
CU329670 Genomic DNA. Translation: CAB10121.1.
PIRiT43236.
RefSeqiNP_594425.1. NM_001019854.2.

Genome annotation databases

EnsemblFungiiSPAC19G12.10c.1; SPAC19G12.10c.1:pep; SPAC19G12.10c.
GeneIDi2542465.
KEGGispo:SPAC19G12.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86560 Genomic DNA. Translation: BAA25568.1 .
CU329670 Genomic DNA. Translation: CAB10121.1 .
PIRi T43236.
RefSeqi NP_594425.1. NM_001019854.2.

3D structure databases

ProteinModelPortali O13849.
SMRi O13849. Positions 572-1000.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 278926. 4 interactions.
IntActi O13849. 1 interaction.
MINTi MINT-4668478.
STRINGi 4896.SPAC19G12.10c-1.

Protein family/group databases

MEROPSi S10.A66.

Proteomic databases

MaxQBi O13849.
PaxDbi O13849.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC19G12.10c.1 ; SPAC19G12.10c.1:pep ; SPAC19G12.10c .
GeneIDi 2542465.
KEGGi spo:SPAC19G12.10c.

Organism-specific databases

PomBasei SPAC19G12.10c.

Phylogenomic databases

eggNOGi COG2939.
KOi K13289.
OMAi GEHMPPP.
OrthoDBi EOG7XDBR1.

Miscellaneous databases

NextBioi 20803521.

Family and domain databases

Gene3Di 3.40.50.1820. 2 hits.
InterProi IPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view ]
PANTHERi PTHR11802. PTHR11802. 1 hit.
Pfami PF00450. Peptidase_S10. 1 hit.
[Graphical view ]
PRINTSi PR00724. CRBOXYPTASEC.
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Vacuolar protein sorting in fission yeast: cloning, biosynthesis, transport, and processing of carboxypeptidase Y from Schizosaccharomyces pombe."
    Tabuchi M., Iwaihara O., Ohtani Y., Ohuchi N., Sakurai J., Morita T., Iwahara S., Takegawa K.
    J. Bacteriol. 179:4179-4189(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-627.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiCBPY_SCHPO
AccessioniPrimary (citable) accession number: O13849
Secondary accession number(s): O14366
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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