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Protein

NAD/NADP-dependent indole-3-acetaldehyde reductase

Gene

SPAC19G12.09

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(Indol-3-yl)ethanol + NAD+ = (indol-3-yl)acetaldehyde + NADH.
(Indol-3-yl)ethanol + NADP+ = (indol-3-yl)acetaldehyde + NADPH.

Kineticsi

  1. KM=34 µM for NADP1 Publication
  2. KM=53.3 µM for NAD1 Publication

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD, NADP

    Enzyme and pathway databases

    SABIO-RKO13848.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD/NADP-dependent indole-3-acetaldehyde reductase (EC:1.1.1.190, EC:1.1.1.191)
    Alternative name(s):
    AKR3C2
    Gene namesi
    ORF Names:SPAC19G12.09
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome I

    Organism-specific databases

    EuPathDBiFungiDB:SPAC19G12.09.
    PomBaseiSPAC19G12.09.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: PomBase
    • cytosol Source: PomBase
    • nucleus Source: PomBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi202 – 2021D → S: 2-fold increase of activity with NADH; no activity with NADPH. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 284284NAD/NADP-dependent indole-3-acetaldehyde reductasePRO_0000339120Add
    BLAST

    Proteomic databases

    MaxQBiO13848.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    MINTiMINT-4668467.

    Structurei

    3D structure databases

    ProteinModelPortaliO13848.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000250272.
    InParanoidiO13848.
    OMAiTTCKEER.
    OrthoDBiEOG092C324N.
    PhylomeDBiO13848.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR020471. Aldo/keto_reductase.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O13848-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLIAAMGPKI PVPAYGVGTA LFKKEKGEIN RTIVDSVKNA LAAGFIHIDC
    60 70 80 90 100
    AEVYGNEEEV GVALKEANVP RSKLFITSKV MHNVDNIPEA LNESLRKLGT
    110 120 130 140 150
    DYLDLYLLHS PIPFYEKKIP ISEGWKAMET ALGTGLVHSV GVSNFRIPDL
    160 170 180 190 200
    EELLKTSTIT PRVNQIEFHP QVYKAAKPLV EFCQSKGIIV EGYGPLSPLV
    210 220 230 240 250
    RDAQGPVAEF TKSLESKYHV SDTQILLKWA YSKGVIPITT TSKIERMKEC
    260 270 280
    LNFDSFTLDK ADIDELGTLG VQHHKRTFMK HMDE
    Length:284
    Mass (Da):31,570
    Last modified:January 1, 1998 - v1
    Checksum:i4B33F1EC1BF14BB8
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAB10120.1.
    PIRiT37996.
    RefSeqiNP_594424.1. NM_001019853.2.

    Genome annotation databases

    EnsemblFungiiSPAC19G12.09.1; SPAC19G12.09.1:pep; SPAC19G12.09.
    GeneIDi2542483.
    KEGGispo:SPAC19G12.09.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAB10120.1.
    PIRiT37996.
    RefSeqiNP_594424.1. NM_001019853.2.

    3D structure databases

    ProteinModelPortaliO13848.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    MINTiMINT-4668467.

    Proteomic databases

    MaxQBiO13848.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiSPAC19G12.09.1; SPAC19G12.09.1:pep; SPAC19G12.09.
    GeneIDi2542483.
    KEGGispo:SPAC19G12.09.

    Organism-specific databases

    EuPathDBiFungiDB:SPAC19G12.09.
    PomBaseiSPAC19G12.09.

    Phylogenomic databases

    HOGENOMiHOG000250272.
    InParanoidiO13848.
    OMAiTTCKEER.
    OrthoDBiEOG092C324N.
    PhylomeDBiO13848.

    Enzyme and pathway databases

    SABIO-RKO13848.

    Miscellaneous databases

    PROiO13848.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red/Kv-b.
    IPR020471. Aldo/keto_reductase.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiI3ACR_SCHPO
    AccessioniPrimary (citable) accession number: O13848
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: January 1, 1998
    Last modified: September 7, 2016
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.