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Protein

E3 ubiquitin-protein ligase complex slx8-rfp subunit rfp1

Gene

rfp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates ubiquitination and subsequent desumoylation/degradation of sumoylated proteins and proteins containing SUMO-like domains. Involved in maintaining genome stability where it acts in the cellular response to DNA damage. Has a role in meiosis.4 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri189 – 238RING-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: PomBase
  • meiotic cell cycle Source: UniProtKB-KW
  • negative regulation of protein sumoylation Source: PomBase
  • protein ubiquitination Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processMeiosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase complex slx8-rfp subunit rfp1 (EC:2.3.2.27)
Alternative name(s):
Meiotically up-regulated gene 140 protein
RING finger protein 1
RING-type E3 ubiquitin transferase rfp1Curated
Gene namesi
Name:rfp1
Synonyms:mug140
ORF Names:SPAC19A8.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC19A8.10.
PomBaseiSPAC19A8.10. rfp1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
  • SUMO-targeted ubiquitin ligase complex Source: PomBase

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563331 – 254E3 ubiquitin-protein ligase complex slx8-rfp subunit rfp1Add BLAST254

Proteomic databases

PRIDEiO13826.

Interactioni

Subunit structurei

Part of an E3 ubiquitin complex including rfp1, rfp2 and slx8. Interacts with slx8, chk1, nse5, pmt3 and rad60.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi278999. 16 interactors.
IntActiO13826. 10 interactors.
MINTiMINT-4668274.

Structurei

3D structure databases

SMRiO13826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi54 – 57Poly-Arg4

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri189 – 238RING-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiO13826.
OrthoDBiEOG092C4VMD.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR009078. Ferritin-like_SF.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF14634. zf-RING_5. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiView protein in PROSITE
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O13826-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFNGSNGID ESSVIDLTRS PSPPVETSIS STNIIDLDAI PDDSFPSSPV
60 70 80 90 100
LSPRRRRMNR RRNERSRNFP SNHLSYLEDM IYLGPQVSTR RSSSRRDLMG
110 120 130 140 150
MIARTFPEFS SVNSLSPSLF QLIVNRMRFD AIHPEWTNGS DDEYFSNHFE
160 170 180 190 200
ESYDDFTSSL ENIKQSYKPP GPPKSGFTRS FNNDTLMVCP RCQEPLGTSK
210 220 230 240 250
SKEKSALWAT KCGHVYCGSC AKVLKTSKRS QSKCLVNDCG RYLNTKNAMW

ELFY
Length:254
Mass (Da):28,903
Last modified:January 1, 1998 - v1
Checksum:iE47B9E2B35E573DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11646.1.
PIRiT37951.
RefSeqiNP_593782.1. NM_001019211.2.

Genome annotation databases

EnsemblFungiiSPAC19A8.10.1; SPAC19A8.10.1:pep; SPAC19A8.10.
GeneIDi2542542.
KEGGispo:SPAC19A8.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11646.1.
PIRiT37951.
RefSeqiNP_593782.1. NM_001019211.2.

3D structure databases

SMRiO13826.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278999. 16 interactors.
IntActiO13826. 10 interactors.
MINTiMINT-4668274.

Proteomic databases

PRIDEiO13826.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC19A8.10.1; SPAC19A8.10.1:pep; SPAC19A8.10.
GeneIDi2542542.
KEGGispo:SPAC19A8.10.

Organism-specific databases

EuPathDBiFungiDB:SPAC19A8.10.
PomBaseiSPAC19A8.10. rfp1.

Phylogenomic databases

InParanoidiO13826.
OrthoDBiEOG092C4VMD.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiPR:O13826.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR009078. Ferritin-like_SF.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF14634. zf-RING_5. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiView protein in PROSITE
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFP1_SCHPO
AccessioniPrimary (citable) accession number: O13826
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: March 15, 2017
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.