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Protein

Cytochrome P450 61

Gene

erg5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required to form the C-22(23) double bond in the sterol side chain.By similarity

Cofactori

hemeBy similarity

Pathwayi: ergosterol biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes ergosterol from zymosterol.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. C-8 sterol isomerase erg2 (erg2)
  3. Delta(7)-sterol 5(6)-desaturase erg31 (erg31), Delta(7)-sterol 5(6)-desaturase erg32 (erg32)
  4. Cytochrome P450 61 (erg5)
  5. Delta(24(24(1)))-sterol reductase (sts1)
This subpathway is part of the pathway ergosterol biosynthesis, which is itself part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ergosterol from zymosterol, the pathway ergosterol biosynthesis and in Steroid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi461Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • ergosterol biosynthetic process Source: PomBase

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism, Stress response
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-211916 Vitamins
R-SPO-5365859 RA biosynthesis pathway
UniPathwayiUPA00768; UER00763

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 61 (EC:1.14.-.-)
Alternative name(s):
C-22 sterol desaturase
Gene namesi
Name:erg5
Synonyms:cyp61By similarity
ORF Names:SPAC19A8.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC19A8.04
PomBaseiSPAC19A8.04 erg5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Does not produce ergosterol, accumulating other sterols.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003344911 – 543Cytochrome P450 61Add BLAST543

Proteomic databases

MaxQBiO13820
PaxDbiO13820
PRIDEiO13820

PTM databases

iPTMnetiO13820

Expressioni

Inductioni

Up-regulated by sre1 in response to absence of oxygen.1 Publication

Interactioni

Subunit structurei

Interacts with dap1.1 Publication

Protein-protein interaction databases

BioGridi278930173 interactors.
STRINGi4896.SPAC19A8.04.1

Structurei

3D structure databases

ProteinModelPortaliO13820
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Sequence analysis

Phylogenomic databases

HOGENOMiHOG000181358
InParanoidiO13820
KOiK09831
OMAiATIFPMD
OrthoDBiEOG092C1MVB

Family and domain databases

Gene3Di1.10.630.101 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

O13820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMNQTETLI PAARNVIRVL GYEIEYSKWT ICFALLAVCI AYDQISYQMQ
60 70 80 90 100
KGHIPGPRFK IPFMGSFLDS MKPTFEKYNA KWQTGPLSCV SVFHKFVVIA
110 120 130 140 150
SERDLARKIL NSPSYVQPCV VDAGKKILKH TNWVFLDGRD HIEYRKGLNG
160 170 180 190 200
LFTTRALASY LPAQEAVYNK YFKEFLAHSK DDYAQYMIPF RDINVATSCR
210 220 230 240 250
TFCGYYISDD AIKHIADEYW KITAAMELVN FPIVLPFTKV WYGIQSRKVV
260 270 280 290 300
MRYFMKAAAE SRKNMEAGNA PACMMEEWIH EMIETRKYKS ENKEGAEKPS
310 320 330 340 350
VLIREFSDEE ISLTFLSFLF ASQDATSSAM TWLFQLLADH PDVLQKVREE
360 370 380 390 400
QLRIRKGDID VPLSLDLMEK MTYTRAVVKE CLRLRPPVLM VPYRVKKAFP
410 420 430 440 450
ITPDYTVPKD AMVIPTLYGA LHDSKVYPEP ETFNPDRWAP NGLAEQSPKN
460 470 480 490 500
WMVFGNGPHV CLGQRYAVNH LIACIGKASI MLDWKHKRTP DSDTQMIFAT
510 520 530 540
TFPQDMCYLK FSPFDASTVD WKNSKEAFSN EAVSAATVET ESA
Length:543
Mass (Da):62,271
Last modified:April 18, 2012 - v3
Checksum:iC0ED03E73532A4AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB11640.3
PIRiT37957
RefSeqiNP_593788.2, NM_001019217.2

Genome annotation databases

EnsemblFungiiSPAC19A8.04.1; SPAC19A8.04.1:pep; SPAC19A8.04
GeneIDi2542469
KEGGispo:SPAC19A8.04

Similar proteinsi

Entry informationi

Entry nameiERG5_SCHPO
AccessioniPrimary (citable) accession number: O13820
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 18, 2012
Last modified: March 28, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome