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Protein

Cytochrome P450 61

Gene

erg5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required to form the C-22(23) double bond in the sterol side chain.By similarity

Cofactori

hemeBy similarity

Pathwayi: ergosterol biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes ergosterol from zymosterol.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Sterol 24-C-methyltransferase erg6 (erg6)
  2. C-8 sterol isomerase erg2 (erg2)
  3. Delta(7)-sterol 5(6)-desaturase erg31 (erg31), Delta(7)-sterol 5(6)-desaturase erg32 (erg32)
  4. Cytochrome P450 61 (erg5)
  5. Delta(24(24(1)))-sterol reductase (sts1)
This subpathway is part of the pathway ergosterol biosynthesis, which is itself part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ergosterol from zymosterol, the pathway ergosterol biosynthesis and in Steroid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi461Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • ergosterol biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism, Stress response

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00768; UER00763.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 61 (EC:1.14.-.-)
Alternative name(s):
C-22 sterol desaturase
Gene namesi
Name:erg5
Synonyms:cyp61By similarity
ORF Names:SPAC19A8.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC19A8.04.
PomBaseiSPAC19A8.04. erg5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Does not produce ergosterol, accumulating other sterols.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003344911 – 543Cytochrome P450 61Add BLAST543

Proteomic databases

MaxQBiO13820.
PRIDEiO13820.

Expressioni

Inductioni

Up-regulated by sre1 in response to absence of oxygen.1 Publication

Interactioni

Subunit structurei

Interacts with dap1.1 Publication

Protein-protein interaction databases

BioGridi278930. 173 interactors.

Structurei

3D structure databases

ProteinModelPortaliO13820.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Sequence analysis

Phylogenomic databases

HOGENOMiHOG000181358.
InParanoidiO13820.
KOiK09831.
OMAiERIIHEH.
OrthoDBiEOG092C1MVB.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMNQTETLI PAARNVIRVL GYEIEYSKWT ICFALLAVCI AYDQISYQMQ
60 70 80 90 100
KGHIPGPRFK IPFMGSFLDS MKPTFEKYNA KWQTGPLSCV SVFHKFVVIA
110 120 130 140 150
SERDLARKIL NSPSYVQPCV VDAGKKILKH TNWVFLDGRD HIEYRKGLNG
160 170 180 190 200
LFTTRALASY LPAQEAVYNK YFKEFLAHSK DDYAQYMIPF RDINVATSCR
210 220 230 240 250
TFCGYYISDD AIKHIADEYW KITAAMELVN FPIVLPFTKV WYGIQSRKVV
260 270 280 290 300
MRYFMKAAAE SRKNMEAGNA PACMMEEWIH EMIETRKYKS ENKEGAEKPS
310 320 330 340 350
VLIREFSDEE ISLTFLSFLF ASQDATSSAM TWLFQLLADH PDVLQKVREE
360 370 380 390 400
QLRIRKGDID VPLSLDLMEK MTYTRAVVKE CLRLRPPVLM VPYRVKKAFP
410 420 430 440 450
ITPDYTVPKD AMVIPTLYGA LHDSKVYPEP ETFNPDRWAP NGLAEQSPKN
460 470 480 490 500
WMVFGNGPHV CLGQRYAVNH LIACIGKASI MLDWKHKRTP DSDTQMIFAT
510 520 530 540
TFPQDMCYLK FSPFDASTVD WKNSKEAFSN EAVSAATVET ESA
Length:543
Mass (Da):62,271
Last modified:April 18, 2012 - v3
Checksum:iC0ED03E73532A4AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11640.3.
PIRiT37957.
RefSeqiNP_593788.2. NM_001019217.2.

Genome annotation databases

EnsemblFungiiSPAC19A8.04.1; SPAC19A8.04.1:pep; SPAC19A8.04.
GeneIDi2542469.
KEGGispo:SPAC19A8.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11640.3.
PIRiT37957.
RefSeqiNP_593788.2. NM_001019217.2.

3D structure databases

ProteinModelPortaliO13820.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278930. 173 interactors.

Proteomic databases

MaxQBiO13820.
PRIDEiO13820.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC19A8.04.1; SPAC19A8.04.1:pep; SPAC19A8.04.
GeneIDi2542469.
KEGGispo:SPAC19A8.04.

Organism-specific databases

EuPathDBiFungiDB:SPAC19A8.04.
PomBaseiSPAC19A8.04. erg5.

Phylogenomic databases

HOGENOMiHOG000181358.
InParanoidiO13820.
KOiK09831.
OMAiERIIHEH.
OrthoDBiEOG092C1MVB.

Enzyme and pathway databases

UniPathwayiUPA00768; UER00763.

Miscellaneous databases

PROiO13820.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERG5_SCHPO
AccessioniPrimary (citable) accession number: O13820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 18, 2012
Last modified: November 30, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.