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Protein

Probable glutamate 5-kinase

Gene

SPAC17H9.13c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline.

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable glutamate 5-kinase (SPAC17H9.13c)
  2. Probable gamma-glutamyl phosphate reductase (pro1)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58SubstrateBy similarity1
Binding sitei145SubstrateBy similarity1
Binding sitei157Substrate; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 178ATPBy similarity2
Nucleotide bindingi218 – 224ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gene namesi
ORF Names:SPAC17H9.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17H9.13c.
PomBaseiSPAC17H9.13c.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097671 – 402Probable glutamate 5-kinaseAdd BLAST402

Proteomic databases

MaxQBiO13810.
PRIDEiO13810.

Interactioni

Protein-protein interaction databases

BioGridi278742. 139 interactors.

Structurei

3D structure databases

ProteinModelPortaliO13810.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini295 – 373PUAAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.Curated
Contains 1 PUA domain.Curated

Phylogenomic databases

HOGENOMiHOG000246369.
InParanoidiO13810.
KOiK00931.
OMAiVIHRDHW.
OrthoDBiEOG092C2KL8.
PhylomeDBiO13810.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13810-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPKSKSTNK TYTIVIKLGT SSICDEKTHE PLISNLSLIV ETVVKLRKLG
60 70 80 90 100
HNVVLVSSGG IAMGLRRLDL PKRPSKLSAV QAIAAVGQGR LISLWDTLFT
110 120 130 140 150
QLRQPIAQVL ITRNDIAERS QYVNAANTIS ELLHFGVVPI VNENDTLSVQ
160 170 180 190 200
EIRFGDNDTL SAITAGMINA DYLFLLTDVD CLYTDNPRTN PDAKPILKIH
210 220 230 240 250
DTSMVNANVS TPGSGVGTGG MKTKLIAADL GTSSGVNVII CRGSKPSSIF
260 270 280 290 300
DIIRQESSDN KNESVELPLH THFVAKKQGR IRDRHFWLLH GLKSHGSLEI
310 320 330 340 350
DRGAFEAITR TNRAGLLPVG VTKVHGHFSA HQAVTVIYNG EEIGRALVNY
360 370 380 390 400
SSTEIDLIKG KRSKEIASIL GYNETEYVAY RDYLVVHGLN SHHDSQVSSD

EH
Length:402
Mass (Da):43,934
Last modified:January 1, 1998 - v1
Checksum:iD78F550F8828B033
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11222.1.
PIRiT37879.
RefSeqiNP_593583.1. NM_001019015.2.

Genome annotation databases

EnsemblFungiiSPAC17H9.13c.1; SPAC17H9.13c.1:pep; SPAC17H9.13c.
GeneIDi2542273.
KEGGispo:SPAC17H9.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11222.1.
PIRiT37879.
RefSeqiNP_593583.1. NM_001019015.2.

3D structure databases

ProteinModelPortaliO13810.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278742. 139 interactors.

Proteomic databases

MaxQBiO13810.
PRIDEiO13810.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC17H9.13c.1; SPAC17H9.13c.1:pep; SPAC17H9.13c.
GeneIDi2542273.
KEGGispo:SPAC17H9.13c.

Organism-specific databases

EuPathDBiFungiDB:SPAC17H9.13c.
PomBaseiSPAC17H9.13c.

Phylogenomic databases

HOGENOMiHOG000246369.
InParanoidiO13810.
KOiK00931.
OMAiVIHRDHW.
OrthoDBiEOG092C2KL8.
PhylomeDBiO13810.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Miscellaneous databases

PROiO13810.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_SCHPO
AccessioniPrimary (citable) accession number: O13810
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.