O13810 (PROB_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamate 5-kinase
Short name=GK
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase

Gene names

ORF Names:

SPAC17H9.13c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline.
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Probable glutamate 5-kinase		PRO_0000109767
Regions
	Domain	295 – 373	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	O13810 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D78F550F8828B033	FASTA	402	43,934
	10 20 30 40 50 60 MSPKSKSTNK TYTIVIKLGT SSICDEKTHE PLISNLSLIV ETVVKLRKLG HNVVLVSSGG 70 80 90 100 110 120 IAMGLRRLDL PKRPSKLSAV QAIAAVGQGR LISLWDTLFT QLRQPIAQVL ITRNDIAERS 130 140 150 160 170 180 QYVNAANTIS ELLHFGVVPI VNENDTLSVQ EIRFGDNDTL SAITAGMINA DYLFLLTDVD 190 200 210 220 230 240 CLYTDNPRTN PDAKPILKIH DTSMVNANVS TPGSGVGTGG MKTKLIAADL GTSSGVNVII 250 260 270 280 290 300 CRGSKPSSIF DIIRQESSDN KNESVELPLH THFVAKKQGR IRDRHFWLLH GLKSHGSLEI 310 320 330 340 350 360 DRGAFEAITR TNRAGLLPVG VTKVHGHFSA HQAVTVIYNG EEIGRALVNY SSTEIDLIKG 370 380 390 400 KRSKEIASIL GYNETEYVAY RDYLVVHGLN SHHDSQVSSD EH

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB11222.1.
PIR	T37879.
RefSeq	NP_593583.1. NM_001019015.1.
3D structure databases
ProteinModelPortal	O13810.
ModBase	Search...
Protein-protein interaction databases
STRING	O13810.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC17H9.13c.1; SPAC17H9.13c.1:pep; SPAC17H9.13c.
GeneID	2542273.
KEGG	spo:SPAC17H9.13c.
NMPDR	fig|4896.1.peg.3553.
Organism-specific databases
GeneDB_Spombe	SPAC17H9.13c.
Phylogenomic databases
eggNOG	fuNOG04245.
GeneTree	EFGT00050000006112.
HOGENOM	HBG507643.
OMA	TFGDNDM.
OrthoDB	EOG4QVGMG.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001257-MONOMER.
Gene expression databases
ArrayExpress	O13810.
Family and domain databases
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_SCHPO

Accession

Primary (citable) accession number: O13810

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program