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Protein

Cullin-1

Gene

cul1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(pop1-pop2) is required for the maintenance of ploidy and directs ubiquitination of cig2.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • negative regulation of mitotic DNA replication initiation Source: PomBase
  • protein ubiquitination Source: UniProtKB-UniPathway
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: PomBase

Keywordsi

Biological processCell cycle, Cell division

Enzyme and pathway databases

ReactomeiR-SPO-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SPO-68949 Orc1 removal from chromatin
R-SPO-8951664 Neddylation
R-SPO-917937 Iron uptake and transport
R-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-1
Short name:
Cul-1
Alternative name(s):
Cell division control 53 homolog
Gene namesi
Name:cul1
Synonyms:pcu1
ORF Names:SPAC17G6.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17G6.12
PomBaseiSPAC17G6.12 cul1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi713K → R: Loss of neddylation; results in impaired cell proliferation and marked stabilization of the cyclin-dependent kinase inhibitor rum1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198081 – 767Cullin-1Add BLAST767

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki713Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)1 Publication

Post-translational modificationi

Neddylated; enhancing the ubiquitin-ligase activity.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiO13790
PaxDbiO13790
PRIDEiO13790

Interactioni

Subunit structurei

Component of multiple SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes formed of cul1, skp1, pip1 and a variable F-box domain-containing protein as substrate-specific subunit (By similarity). Binds to the pop1 homodimer, the pop2 homodimer and the pop1/pop2 heterodimer forming the SCF(pop1-pop2) complex. Interacts with pof3, pof14 and skp1.By similarity4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi278857, 30 interactors
IntActiO13790, 7 interactors
MINTiO13790
STRINGi4896.SPAC17G6.12.1

Structurei

3D structure databases

ProteinModelPortaliO13790
SMRiO13790
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi263 – 266Poly-Glu4
Compositional biasi444 – 447Poly-Ile4

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000176713
InParanoidiO13790
KOiK03347
OMAiTTHKHIE
OrthoDBiEOG092C1L4K
PhylomeDBiO13790

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR016159 Cullin_repeat-like_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00888 Cullin, 1 hit
PF10557 Cullin_Nedd8, 1 hit
SMARTiView protein in SMART
SM00182 CULLIN, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF74788 SSF74788, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

O13790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLNTNDKD LPIVKKYDSL NGTWDFLKTG VSQIFERLDE GMTITKYMEL
60 70 80 90 100
YTAIHNYCAD ASKTITVDNF NDQTANVLGE ALYNNLVLYL EEYLARLRKE
110 120 130 140 150
CISQTNHEEQ LAAYAKYWTR FTTSARFINH LFGYLNRYWV KLKNRFTETL
160 170 180 190 200
VYDIYTLCLV SWHHHVFSHI RDSLLQNLLY MFTKKRLYEP TDMKYVEVCV
210 220 230 240 250
DSITSLSFDK TDMTKPNLSS YKTFFETNFI ENTKNFYAKE SSEYLASHSI
260 270 280 290 300
TDYLKKAEIR LAEEEELVRL YLHESTLKPL LEATEDVLIA QHEEVLHNDF
310 320 330 340 350
ARMLDQNCSE DIIRMYRLMS RTPNGLQPLR QTFEEFVKRS GFAAVAKIVP
360 370 380 390 400
QVGGEADVDP KEYMEMLLST YKASKELVNT AFHGDTDFTK SLDTAFRELV
410 420 430 440 450
NRNVVCQRSS SRSPELLAKY ADSILRKSNK NVDIDDVEDC LSSIIIIFRY
460 470 480 490 500
VEDKDVFQNF YTKLLAKRLV NGTSNSQDAE SSMLSKLKEV CGFEYTSKLQ
510 520 530 540 550
RMFQDISLSQ EITEAFWQLP QSRAGNIDFS ALVLGTSFWP LSPNNVNFHL
560 570 580 590 600
PEELVPLYEG FQNYYYSCHN GRKLSWLFHL SKGEIKARIN PQTNVTYVFQ
610 620 630 640 650
VSTYQMGVLL LYNHRDSYTY EELAKITGLS TDFLTGILNI FLKAKVLLLG
660 670 680 690 700
DNDKLGDPNS TYKINENFRM KKIRVQLNLP IRSEQKQESL ETHKTIEEDR
710 720 730 740 750
KLLLQSAIVR IMKARRTLKH VVLVKETIDQ IKSRFTPKVS DIKQCIDMLI
760
EKEYLERQGR DEYIYLA
Length:767
Mass (Da):89,426
Last modified:January 1, 1998 - v1
Checksum:i458CA31B5D97FAA9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23Missing in BAA32428 (PubMed:9990507).Curated1
Sequence conflicti52T → TR in BAA32428 (PubMed:9990507).Curated1
Sequence conflicti74T → A in BAA32428 (PubMed:9990507).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016896 Genomic DNA Translation: BAA32428.2
CU329670 Genomic DNA Translation: CAB16223.1
AB027897 Genomic DNA Translation: BAA87201.1
PIRiT37844
T43398
RefSeqiNP_594259.1, NM_001019682.2

Genome annotation databases

EnsemblFungiiSPAC17G6.12.1; SPAC17G6.12.1:pep; SPAC17G6.12
GeneIDi2542393
KEGGispo:SPAC17G6.12

Similar proteinsi

Entry informationi

Entry nameiCUL1_SCHPO
AccessioniPrimary (citable) accession number: O13790
Secondary accession number(s): Q9USB3, Q9UUL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families
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