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Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

gus1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPBy similarity1
Binding sitei246GlutamateBy similarity1
Binding sitei404GlutamateBy similarity1
Binding sitei407ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi441 – 445ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gus1
Synonyms:ers1
ORF Names:SPAC17A5.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17A5.15c.
PomBaseiSPAC17A5.15c. gus1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • mitochondrion Source: GO_Central
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197381 – 716Probable glutamate--tRNA ligase, cytoplasmicAdd BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei190Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO13775.
PRIDEiO13775.

PTM databases

iPTMnetiO13775.

Interactioni

Subunit structurei

Component of a yeast aminoacyl-tRNA synthase (aaRS) complex formed by methionyl-tRNA synthase, glutamyl-tRNA synthase and the tRNA aminoacylation cofactor arc1 in a stoichiometric complex. Interacts with arc1/SPAC30C2.04 (By similarity).By similarity

Protein-protein interaction databases

BioGridi278872. 2 interactors.
MINTiMINT-4667855.

Structurei

3D structure databases

ProteinModelPortaliO13775.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 212Glutamate bindingBy similarity3
Regioni386 – 390Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi215 – 224"HIGH" region10
Motifi441 – 445"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000259234.
InParanoidiO13775.
KOiK01885.
OMAiDYFDYLY.
OrthoDBiEOG092C0OTH.
PhylomeDBiO13775.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
1.20.1050.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR010987. Glutathione-S-Trfase_C-like.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSVALKAK PIAYGAVACA NYVNLSGAGS ISVKYEDVAL LDKQNKESNV
60 70 80 90 100
SIQLNGSDSP VFGSKLALQT FSQVYPKLFI GENDRSLVES WVETASALAG
110 120 130 140 150
NHNFLELSSL LAQLDDHLIM RSLFVGYSLT SADFSIWGAL KSNNMAAGAV
160 170 180 190 200
RTGQYFNLAR WYKFMDSQNA VSVTMEEFTK AVNISKKQKS SGPNYEIGLP
210 220 230 240 250
DAIDGKVVTR FPPEPSGYLH IGHAKAALLN QYFANKYHGK LIVRFDDTNP
260 270 280 290 300
SKENSEFQDA ILEDVALLGI KPDVVTYTSD YLDTIHQYCV DMIKSGQAYA
310 320 330 340 350
DDTDVETMRH ERTEGIPSKH RDRPIEESLE ILSEMDKGSD VGLKNCIRAK
360 370 380 390 400
ISYENPNKAM RDPVIYRCNL LPHHRTGTKY RAYPTYDFAC PIVDSLEGVT
410 420 430 440 450
HALRTTEYRD RNPLYQWMIK AMNLRKIHVW EFSRMNFVRT LLSKRKLTEI
460 470 480 490 500
VDHGLVWGWD DPRFPTVRGV RRRGMTIEAL QQYIVSQGPS KNILTLDWTS
510 520 530 540 550
FWATNKKIID PVAPRHTAVE SGDVVKATIV NGPAAPYAED RPRHKKNPEL
560 570 580 590 600
GNKKSIFANE ILIEQADAQS FKQDEEVTLM DWGNAYVREI NRDASGKVTS
610 620 630 640 650
LKLELHLDGD FKKTEKKVTW LADTEDKTPV DLVDFDYLIT KDKLEEGENY
660 670 680 690 700
KDFLTPQTEF HSPVFADVGI KNLKKGDIIQ VERKGYYIVD VPFDGTQAVL
710
FNIPDGKTVN RYGVKN
Length:716
Mass (Da):80,750
Last modified:January 1, 1998 - v1
Checksum:i2ACE0A35ED393227
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11515.1.
PIRiT37830.
RefSeqiNP_593483.1. NM_001018916.2.

Genome annotation databases

EnsemblFungiiSPAC17A5.15c.1; SPAC17A5.15c.1:pep; SPAC17A5.15c.
GeneIDi2542408.
KEGGispo:SPAC17A5.15c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11515.1.
PIRiT37830.
RefSeqiNP_593483.1. NM_001018916.2.

3D structure databases

ProteinModelPortaliO13775.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278872. 2 interactors.
MINTiMINT-4667855.

PTM databases

iPTMnetiO13775.

Proteomic databases

MaxQBiO13775.
PRIDEiO13775.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC17A5.15c.1; SPAC17A5.15c.1:pep; SPAC17A5.15c.
GeneIDi2542408.
KEGGispo:SPAC17A5.15c.

Organism-specific databases

EuPathDBiFungiDB:SPAC17A5.15c.
PomBaseiSPAC17A5.15c. gus1.

Phylogenomic databases

HOGENOMiHOG000259234.
InParanoidiO13775.
KOiK01885.
OMAiDYFDYLY.
OrthoDBiEOG092C0OTH.
PhylomeDBiO13775.

Miscellaneous databases

PROiO13775.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
1.20.1050.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR010987. Glutathione-S-Trfase_C-like.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYEC_SCHPO
AccessioniPrimary (citable) accession number: O13775
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.