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Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

gus1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPBy similarity1
Binding sitei246GlutamateBy similarity1
Binding sitei404GlutamateBy similarity1
Binding sitei407ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi441 – 445ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gus1
Synonyms:ers1
ORF Names:SPAC17A5.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17A5.15c
PomBaseiSPAC17A5.15c gus1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197381 – 716Probable glutamate--tRNA ligase, cytoplasmicAdd BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei190Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO13775
PaxDbiO13775
PRIDEiO13775

PTM databases

iPTMnetiO13775

Interactioni

Subunit structurei

Component of a yeast aminoacyl-tRNA synthase (aaRS) complex formed by methionyl-tRNA synthase, glutamyl-tRNA synthase and the tRNA aminoacylation cofactor arc1 in a stoichiometric complex. Interacts with arc1/SPAC30C2.04 (By similarity).By similarity

Protein-protein interaction databases

BioGridi278872, 3 interactors
STRINGi4896.SPAC17A5.15c.1

Structurei

3D structure databases

ProteinModelPortaliO13775
SMRiO13775
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 212Glutamate bindingBy similarity3
Regioni386 – 390Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi215 – 224"HIGH" region10
Motifi441 – 445"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000259234
InParanoidiO13775
KOiK01885
OMAiFARMNFI
OrthoDBiEOG092C0OTH
PhylomeDBiO13775

Family and domain databases

Gene3Di2.40.240.10, 3 hits
3.40.50.620, 1 hit
HAMAPiMF_02076 Glu_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004526 Glu-tRNA-synth_arc/euk
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR036282 Glutathione-S-Trfase_C_sf
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF47616 SSF47616, 1 hit
SSF50715 SSF50715, 1 hit
TIGRFAMsiTIGR00463 gltX_arch, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

O13775-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSVALKAK PIAYGAVACA NYVNLSGAGS ISVKYEDVAL LDKQNKESNV
60 70 80 90 100
SIQLNGSDSP VFGSKLALQT FSQVYPKLFI GENDRSLVES WVETASALAG
110 120 130 140 150
NHNFLELSSL LAQLDDHLIM RSLFVGYSLT SADFSIWGAL KSNNMAAGAV
160 170 180 190 200
RTGQYFNLAR WYKFMDSQNA VSVTMEEFTK AVNISKKQKS SGPNYEIGLP
210 220 230 240 250
DAIDGKVVTR FPPEPSGYLH IGHAKAALLN QYFANKYHGK LIVRFDDTNP
260 270 280 290 300
SKENSEFQDA ILEDVALLGI KPDVVTYTSD YLDTIHQYCV DMIKSGQAYA
310 320 330 340 350
DDTDVETMRH ERTEGIPSKH RDRPIEESLE ILSEMDKGSD VGLKNCIRAK
360 370 380 390 400
ISYENPNKAM RDPVIYRCNL LPHHRTGTKY RAYPTYDFAC PIVDSLEGVT
410 420 430 440 450
HALRTTEYRD RNPLYQWMIK AMNLRKIHVW EFSRMNFVRT LLSKRKLTEI
460 470 480 490 500
VDHGLVWGWD DPRFPTVRGV RRRGMTIEAL QQYIVSQGPS KNILTLDWTS
510 520 530 540 550
FWATNKKIID PVAPRHTAVE SGDVVKATIV NGPAAPYAED RPRHKKNPEL
560 570 580 590 600
GNKKSIFANE ILIEQADAQS FKQDEEVTLM DWGNAYVREI NRDASGKVTS
610 620 630 640 650
LKLELHLDGD FKKTEKKVTW LADTEDKTPV DLVDFDYLIT KDKLEEGENY
660 670 680 690 700
KDFLTPQTEF HSPVFADVGI KNLKKGDIIQ VERKGYYIVD VPFDGTQAVL
710
FNIPDGKTVN RYGVKN
Length:716
Mass (Da):80,750
Last modified:January 1, 1998 - v1
Checksum:i2ACE0A35ED393227
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB11515.1
PIRiT37830
RefSeqiNP_593483.1, NM_001018916.2

Genome annotation databases

EnsemblFungiiSPAC17A5.15c.1; SPAC17A5.15c.1:pep; SPAC17A5.15c
GeneIDi2542408
KEGGispo:SPAC17A5.15c

Similar proteinsi

Entry informationi

Entry nameiSYEC_SCHPO
AccessioniPrimary (citable) accession number: O13775
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

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