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O13775

- SYEC_SCHPO

UniProt

O13775 - SYEC_SCHPO

Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

gus1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATPBy similarity
    Binding sitei246 – 2461GlutamateBy similarity
    Binding sitei404 – 4041GlutamateBy similarity
    Binding sitei407 – 4071ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi441 – 4455ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-tRNA ligase activity Source: PomBase
    3. protein binding Source: PomBase
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. cytoplasmic translation Source: PomBase
    2. glutamyl-tRNA aminoacylation Source: PomBase

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase
    Short name:
    GluRS
    Gene namesi
    Name:gus1
    Synonyms:ers1
    ORF Names:SPAC17A5.15c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC17A5.15c.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 716716Probable glutamate--tRNA ligase, cytoplasmicPRO_0000119738Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei190 – 1901Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO13775.
    PaxDbiO13775.

    Interactioni

    Subunit structurei

    Component of a yeast aminoacyl-tRNA synthase (aaRS) complex formed by methionyl-tRNA synthase, glutamyl-tRNA synthase and the tRNA aminoacylation cofactor arc1 in a stoichiometric complex. Interacts with arc1/SPAC30C2.04 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi278872. 4 interactions.
    MINTiMINT-4667855.
    STRINGi4896.SPAC17A5.15c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO13775.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni210 – 2123Glutamate bindingBy similarity
    Regioni386 – 3905Glutamate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi215 – 22410"HIGH" region
    Motifi441 – 4455"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000259234.
    KOiK01885.
    OMAiTDRNPQY.
    OrthoDBiEOG7MKWFN.
    PhylomeDBiO13775.

    Family and domain databases

    Gene3Di1.10.1160.10. 1 hit.
    1.20.1050.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR004526. Glu-tRNA-synth_arc/euk.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR010987. Glutathione-S-Trfase_C-like.
    IPR004046. GST_C.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF14497. GST_C_3. 1 hit.
    PF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF50715. SSF50715. 1 hit.
    TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O13775-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVSVALKAK PIAYGAVACA NYVNLSGAGS ISVKYEDVAL LDKQNKESNV    50
    SIQLNGSDSP VFGSKLALQT FSQVYPKLFI GENDRSLVES WVETASALAG 100
    NHNFLELSSL LAQLDDHLIM RSLFVGYSLT SADFSIWGAL KSNNMAAGAV 150
    RTGQYFNLAR WYKFMDSQNA VSVTMEEFTK AVNISKKQKS SGPNYEIGLP 200
    DAIDGKVVTR FPPEPSGYLH IGHAKAALLN QYFANKYHGK LIVRFDDTNP 250
    SKENSEFQDA ILEDVALLGI KPDVVTYTSD YLDTIHQYCV DMIKSGQAYA 300
    DDTDVETMRH ERTEGIPSKH RDRPIEESLE ILSEMDKGSD VGLKNCIRAK 350
    ISYENPNKAM RDPVIYRCNL LPHHRTGTKY RAYPTYDFAC PIVDSLEGVT 400
    HALRTTEYRD RNPLYQWMIK AMNLRKIHVW EFSRMNFVRT LLSKRKLTEI 450
    VDHGLVWGWD DPRFPTVRGV RRRGMTIEAL QQYIVSQGPS KNILTLDWTS 500
    FWATNKKIID PVAPRHTAVE SGDVVKATIV NGPAAPYAED RPRHKKNPEL 550
    GNKKSIFANE ILIEQADAQS FKQDEEVTLM DWGNAYVREI NRDASGKVTS 600
    LKLELHLDGD FKKTEKKVTW LADTEDKTPV DLVDFDYLIT KDKLEEGENY 650
    KDFLTPQTEF HSPVFADVGI KNLKKGDIIQ VERKGYYIVD VPFDGTQAVL 700
    FNIPDGKTVN RYGVKN 716
    Length:716
    Mass (Da):80,750
    Last modified:January 1, 1998 - v1
    Checksum:i2ACE0A35ED393227
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11515.1.
    PIRiT37830.
    RefSeqiNP_593483.1. NM_001018916.2.

    Genome annotation databases

    EnsemblFungiiSPAC17A5.15c.1; SPAC17A5.15c.1:pep; SPAC17A5.15c.
    GeneIDi2542408.
    KEGGispo:SPAC17A5.15c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB11515.1 .
    PIRi T37830.
    RefSeqi NP_593483.1. NM_001018916.2.

    3D structure databases

    ProteinModelPortali O13775.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 278872. 4 interactions.
    MINTi MINT-4667855.
    STRINGi 4896.SPAC17A5.15c-1.

    Proteomic databases

    MaxQBi O13775.
    PaxDbi O13775.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC17A5.15c.1 ; SPAC17A5.15c.1:pep ; SPAC17A5.15c .
    GeneIDi 2542408.
    KEGGi spo:SPAC17A5.15c.

    Organism-specific databases

    PomBasei SPAC17A5.15c.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000259234.
    KOi K01885.
    OMAi TDRNPQY.
    OrthoDBi EOG7MKWFN.
    PhylomeDBi O13775.

    Miscellaneous databases

    NextBioi 20803467.

    Family and domain databases

    Gene3Di 1.10.1160.10. 1 hit.
    1.20.1050.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR004526. Glu-tRNA-synth_arc/euk.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR010987. Glutathione-S-Trfase_C-like.
    IPR004046. GST_C.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF14497. GST_C_3. 1 hit.
    PF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF50715. SSF50715. 1 hit.
    TIGRFAMsi TIGR00463. gltX_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-190, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiSYEC_SCHPO
    AccessioniPrimary (citable) accession number: O13775
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 6, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3