O13755 (FRDS_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative fumarate reductase

Alternative name(s):
FAD-dependent oxidoreductase FRDS
NADH
EC=1.3.1.6
NADH-dependent fumarate reductase

Gene names

ORF Names:

SPAC17A2.05

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Could be a fumarate reductase.
Catalytic activity	Succinate + NAD+ = fumarate + NADH.
Cofactor	FAD.
Subcellular location	Cytoplasm. Mitochondrion. Nucleus Ref.2.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion Nucleus
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe tricarboxylic acid cycle Inferred by curator. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe mitochondrion Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro fumarate reductase (NADH) activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe succinate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	Putative fumarate reductase		PRO_0000316583
Regions
	Nucleotide binding	41 – 55	15	AMP Potential
Sites
	Active site	288	1	By similarity
	Active site	311	1	By similarity
Amino acid modifications
	Modified residue	100	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O13755 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9906AD63A570EC11	FASTA	513	55,484
	10 20 30 40 50 60 MRCLTIYTWT FRRLPFIPST NSASFFSTLR FNMSTANNTQ AIVIGGGLAG LSATNTILDL 70 80 90 100 110 120 GGNVLLLDKN TAFGGNSVKA ASGINAAPTQ LQFDQHVSDS VNTFYNDSIL SAKSKAKPEL 130 140 150 160 170 180 LRTLTSKSSS AVDWLSERFG LQMDQLSRLA GHSEPRTHRG THPDYPFKPL AFVLVDQTEK 190 200 210 220 230 240 FAASHPDRLQ IKKNARVTRL LTNPNHDKVF GVEYMDLSDK SNHTVYGPVV LATGGYAADY 250 260 270 280 290 300 SDDSLLKLYH PEALSLSTTN GPYCTGDGHK MVMSIGGSTV DLDLVQIHPT GFVDPKDPTA 310 320 330 340 350 360 LTKFLAAEAL RGSGAVLLTS QGRRFCDELG YRDYVTGEMM KLKSPVYLVL NSAAAEEVAN 370 380 390 400 410 420 FIKFYSFKGL MKKMKAEELC STLNCTKDEL ASTFSEYNRA AKGEIPDEFG RKYFGKTPLE 430 440 450 460 470 480 LTDTFTVGEV VPVLHYTMGG VQVDTQSRVL STNGNVIDGL FAAGEIVGGI HGENRLGGSS 490 500 510 LLACVVFGRL AGQGASSTML RRFIASSTST ASS

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB16560.1.
PIR	T37806.
RefSeq	NP_594239.1. NM_001019662.1.
3D structure databases
HSSP	HSSP built from PDB template 1D4D based on UniProtKB P83223.
ProteinModelPortal	O13755.
ModBase	Search...
Protein-protein interaction databases
STRING	O13755.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC17A2.05.1; SPAC17A2.05.1:pep; SPAC17A2.05.
GeneID	2542206.
KEGG	spo:SPAC17A2.05.
NMPDR	fig|4896.1.peg.4209.
Organism-specific databases
GeneDB_Spombe	SPAC17A2.05.
Phylogenomic databases
eggNOG	fuNOG04455.
GeneTree	EFGT00050000001028.
HOGENOM	HBG678063.
OMA	PVLHYTM.
OrthoDB	EOG43V340.
Gene expression databases
ArrayExpress	O13755.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR010960. Flavocytochrome_c. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01813. Flavo_cyto_c. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FRDS_SCHPO

Accession

Primary (citable) accession number: O13755

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program