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Protein

General transcription and DNA repair factor IIH subunit tfb1

Gene

tfb1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-SPO-113418 Formation of the Early Elongation Complex
R-SPO-5696395 Formation of Incision Complex in GG-NER
R-SPO-5696400 Dual Incision in GG-NER
R-SPO-674695 RNA Polymerase II Pre-transcription Events
R-SPO-6781823 Formation of TC-NER Pre-Incision Complex
R-SPO-6782135 Dual incision in TC-NER
R-SPO-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SPO-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SPO-72086 mRNA Capping
R-SPO-73776 RNA Polymerase II Promoter Escape
R-SPO-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SPO-75953 RNA Polymerase II Transcription Initiation
R-SPO-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SPO-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription and DNA repair factor IIH subunit tfb1
Short name:
TFIIH subunit tfb1
Alternative name(s):
RNA polymerase II transcription factor B 73 kDa subunit
RNA polymerase II transcription factor B p73 subunit
RNA polymerase II transcription factor B subunit 1
Gene namesi
Name:tfb1
ORF Names:SPAC16E8.11c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC16E8.11c
PomBaseiSPAC16E8.11c tfb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192601 – 533General transcription and DNA repair factor IIH subunit tfb1Add BLAST533

Proteomic databases

MaxQBiO13745
PaxDbiO13745
PRIDEiO13745

PTM databases

iPTMnetiO13745

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/ptr8, XPD/rad15, ssl1, tfb1, tfb2, tfb4 and tfb5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of mcs2/cyclin H, mcs6/cdk7 and pmh1/tfb3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.1 Publication

Protein-protein interaction databases

BioGridi278802, 3 interactors
IntActiO13745, 1 interactor
STRINGi4896.SPAC16E8.11c.1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 169BSD 1PROSITE-ProRule annotationAdd BLAST54
Domaini190 – 241BSD 2PROSITE-ProRule annotationAdd BLAST52

Sequence similaritiesi

Belongs to the TFB1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiO13745
KOiK03141
OMAiWSRFFLS
OrthoDBiEOG092C337H

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR005607 BSD_dom
IPR011993 PH-like_dom_sf
IPR027079 Tfb1/GTF2H1
IPR013876 TFIIH_BTF_p62_N
PANTHERiPTHR12856 PTHR12856, 1 hit
PfamiView protein in Pfam
PF03909 BSD, 2 hits
PF08567 PH_TFIIH, 1 hit
SMARTiView protein in SMART
SM00751 BSD, 2 hits
PROSITEiView protein in PROSITE
PS50858 BSD, 2 hits

Sequencei

Sequence statusi: Complete.

O13745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDRVEALAI FRKKQGVLSI DSRLKWTGEG KTTPSVDIAF DAISNLQTTP
60 70 80 90 100
ASNPKVMIRV FIVVKEGEDP TSLVFHFTGT PNARENCDMI TNELRNAIQR
110 120 130 140 150
QREGSQSKPQ GANVDRVNST NLEKDIDLQE SLLTNNPDLL QTFKEAVMKG
160 170 180 190 200
HLSNEQFWST RLHLLRAHAV ERSQQRGPYN VLSTIKPKTV DNQMKVSLTG
210 220 230 240 250
QQIHDMFEQH PLLRKVYDKH VPPLAEGEFW SRFFLSKLCK KLRGDRITPM
260 270 280 290 300
DPSDDIMDKY LKDNEEVTKV SDEPIASHLF DLEGNDQNAN VIAELRPDIT
310 320 330 340 350
MRIDKEALPF MKNINQLSER LLEKSLGNSK RFNNENEETY LKESGFHDLE
360 370 380 390 400
EEASDSKVVL KIKGQDQLLE NNFVPSKNQV GLEPLPSLEA LQHLYQEDSI
410 420 430 440 450
SLNHIEHDSD SLAEAAMQLT QSMREKHEFE THGTASNLPI DIKNEIVLCH
460 470 480 490 500
TVTIEFLHQF WNALLNTTFP DKKAMAPLQN ALLNSKKRVE AIASQAQEQN
510 520 530
VDPKLVYELV SCTLTSIDTS ISEYQRRMSY PPA
Length:533
Mass (Da):60,600
Last modified:March 21, 2012 - v2
Checksum:iA042EFFA0B4B469D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB11039.2
PIRiT37791
RefSeqiNP_594223.2, NM_001019646.2

Genome annotation databases

EnsemblFungiiSPAC16E8.11c.1; SPAC16E8.11c.1:pep; SPAC16E8.11c
GeneIDi2542336
KEGGispo:SPAC16E8.11c

Similar proteinsi

Entry informationi

Entry nameiTFB1_SCHPO
AccessioniPrimary (citable) accession number: O13745
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: March 21, 2012
Last modified: March 28, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health